1996
DOI: 10.1093/nar/24.15.2959
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Participation of altered upstream stimulatory factor in the induction of rat heme oxygenase-1 by cadmium

Abstract: We have reported that an upstream stimulatory factor (USF) binding site is functional in transcription of the heme oxygenase-1 gene. In this study, we examined the role of USF in the induced state. By transient expression analyses with the chloramphenicol acetyl-transferase gene, we found that the USF binding site plays an important role in the induction of rat heme oxygenase-1 by cadmium, but not by hemin. To elucidate the role of USF, we prepared USF-rich nuclear extracts from control and cadmium-treated rat… Show more

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Cited by 31 publications
(19 citation statements)
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“…The identity of the Ebox binding protein as USF-1 was established using ChIP analyses, and thus the data suggest that USF-1 may be a target of ROS-induced signaling. Indeed, precedents for the involvement of USF-1 in oxidative stressmediated changes in gene expression have been previously established (4,31). In theory, an interaction between USF-1 and CREB, either directly or indirectly via the interaction with CREB-recruited coactivators, could promote the ROS effect on PGC-1␣ promoter activity leading to the ultimate upregulation of PGC-1␣ mRNA.…”
Section: Discussionmentioning
confidence: 99%
“…The identity of the Ebox binding protein as USF-1 was established using ChIP analyses, and thus the data suggest that USF-1 may be a target of ROS-induced signaling. Indeed, precedents for the involvement of USF-1 in oxidative stressmediated changes in gene expression have been previously established (4,31). In theory, an interaction between USF-1 and CREB, either directly or indirectly via the interaction with CREB-recruited coactivators, could promote the ROS effect on PGC-1␣ promoter activity leading to the ultimate upregulation of PGC-1␣ mRNA.…”
Section: Discussionmentioning
confidence: 99%
“…An extended AP-1-like motif (19), dubbed the stress response element (or StRE) and recently shown to interact preferentially with Cap'n'Collar transcription factor family members (4), has also been implicated in signaling to HO-1 transcription by both oxidative stressors and antioxidants (4). Binding of an upstream stimulatory factor to a site immediately upstream (Ϫ51 to Ϫ35 bp) of the HO-1 transcriptional start site has also been implicated in HO-1 gene regulation in response to cadmium (25,35).…”
Section: Discussionmentioning
confidence: 99%
“…Annealed oligonucleotides were diluted to 25 ng/l in STE buffer. The 5Ј-end labeling was performed with T4 polynucleotide kinase (Promega) using 25 ng of double-stranded oligonucleotide and 25 Ci of [␥- 32 P]ATP (3000 Ci/ mmol; Amersham Biosciences, Little Chalfont, Buckinghamshire, United Kingdom). The labeled probes were purified using a Sephadex G-25 spin column (Amersham Biosciences).…”
Section: Methodsmentioning
confidence: 99%
“…Among the large variety of putative regulatory sites found in the promoter 5Ј-flanking region of human, rat, mouse, and chicken HO-1 genes (29), most investigations have focused on those that exhibit inducible responses to oxidative signals (30). Several AP-1-binding sites have been shown to be involved in the transcriptional induction of HO-1 by MAPKs, cyclic nucleotides, protein phosphatase inhibitors, and the polyphenol curcumin (31)(32)(33).…”
mentioning
confidence: 99%