Participation of an Intermediate of Oxidative Phosphorylation in Ion Accumulation by Mitochondria

Brierley, Gerald P.; Mürer, Erik H.; Green, D. E.

doi:10.1126/science.140.3562.60

Cited by 110 publications

(28 citation statements)

References 6 publications

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“…Hence, the amount of cation bound within and without some exchange barrier (probably the outer cell membrane) can be deuced to be a function of metabolism involving high energy phosphate. Recent reports showing increased Ca binding in mitochondria at the expense of ATP are of interest in this respect (2,3,6,24 (20). In the important respects we have confirmed his observations on the effects of ultraviolet light and ribonuclease.…”

Section: Discussionsupporting

confidence: 81%

Ion Uptake by Soybean Root Tissue Depleted of Calcium by Ethylenediaminetetraacetic Acid

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Hanson

1964

Plant Physiol.

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Section: Discussionsupporting

confidence: 81%

Ion Uptake by Soybean Root Tissue Depleted of Calcium by Ethylenediaminetetraacetic Acid

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Hanson

1964

Plant Physiol.

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“…The energy-dependent calcium uptake by isolated mitochondria is supported by either the oxidation of respiratory substrates, or by the hydrolysis of added ATP (Rossi & Lehninger, 1963;Brierley, Murer & Bachmann, 1964;Bielawski & Lehninger, 1966;Malmstr6m & Carafoli, 1977). In the first case the process is inhibited by respiratory inhibitors and not by oligomycin (Vasington & Murphy, 1962;Brierley, Murer & Green, 1963;Brierley et al, 1964), in the second, by oligomycin, and not by respiratory inhibitors (Rossi & Lehninger, 1963;Brierley et al, 1964;Bielawski & Lehninger, 1966). In both cases the transport is abolished by uncouplers of oxidative phosphorylation (Vasington & Murphy, 1962;Brierley et al, 1963;Bielawski & Lehninger, 1966).…”

Section: Incubation Proceduresmentioning

confidence: 99%

“…In the first case the process is inhibited by respiratory inhibitors and not by oligomycin (Vasington & Murphy, 1962;Brierley, Murer & Green, 1963;Brierley et al, 1964), in the second, by oligomycin, and not by respiratory inhibitors (Rossi & Lehninger, 1963;Brierley et al, 1964;Bielawski & Lehninger, 1966). In both cases the transport is abolished by uncouplers of oxidative phosphorylation (Vasington & Murphy, 1962;Brierley et al, 1963;Bielawski & Lehninger, 1966). The role of endogenous mast cell ATP in the release of histamine by A23187 and extracellular calcium has also been studied under anaerobic conditions by the use of antimycin A, which blocks the respiration and thus the mitochondrial calcium uptake.…”

Section: Incubation Proceduresmentioning

confidence: 99%

Utilization of Adenosine Triphosphate in Rat Mast Cells During Histamine Release Induced by the Ionophore A23187

Johansen¹

1979

British J Pharmacology

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1 The role of endogenous adenosine triphosphate (ATP) in histamine release from rat mast cells induced by the ionophore A23187 in vitro has been studied. 2 The amount of histamine released by calcium from rat mast cells primed with the ionophore A23187 was dependent on the ATP content of the mast cells. 3 In aerobic experiments a drastic reduction in mast cell ATP content was found during the time when histamine release induced by A23187 takes place. 4 Anaerobic experiments were performed with metabolic inhibitors (antimycin A, oligomycin, and carbonyl cyanide p-trifluorometroxyphenylhydrazone), which are known to block the energy-dependent calcium uptake by isolated mitochondria. The mast cell ATP content was reduced during A23187-induced histamine release under anaerobic conditions in the presence of glucose. This indicates an increased utilization of ATP during the release process. 5 The observations are consistent with the view that energy requiring processes are involved in ionophore-induced histamine release from rat mast cells although part of the ATP reduction in the aerobic experiments may be due to an uncoupling effect of calcium on the oxidative phosphorylation. IntroductionThe antibiotic A23187 specifically forms complexes with calcium and magnesium and transports these ions across a variety of membranes (Reed & Lardy, 1972). In the presence of calcium, A23187 acts as a histamine releasing agent on rat isolated mast cells (Foreman, Mongar & Gomperts, 1973).Results of a study of the dependence of A23187-induced histamine release on calcium and temperature, and a time course similar to the anaphylactic histamine release, indicate that the same mechanism may be involved in anaphylactic and A23187-induced histamine release (Johansen, 1978).Anaphylactic histamine release is dependent on mast cell ATP content and there is an increased utilization of ATP during the release (Johansen & Chakravarty, 1975). A23187-induced histamine release has also been shown to be blocked by preincubation of the cells with metabolic inhibitors (Foreman et al., 1973).In the present investigation the relation between mast cell ATP content and A23187-induced histamine release under aerobic and anaerobic conditions has been studied in order to evaluate the dependence of the histamine release mechanism on endogenous ATP. Methods Isolation of rat mast cellsMale Sprague-Dawley rats, 285 to 550 g, were used for the experiments. Mast cells were isolated by differential centrifugation in concentrated human serum albumin by a slight modification of the method described by Chakravarty (1965) Incubation procedureMast cell suspensions pooled from 2 to 6 rats were divided into samples of the same cell density in a final volume of 0.5 ml both for determination of the ATP content of the mast cells and for the histamine release experiments. The cell density from different experiments varied from 0.8 x 105 to 2.6 x 105 cells per ml. The cell suspensions were prewarmed in a 37°C bath for 10 min and the incubation was continued with A23187 (10 j...

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“…The "active" or respiration-linked accumulation of Ca ++ from the suspending medium by isolated mitochondria, first demonstrated by Vasington and Murphy (1,2), has been studied in some detail in a number of recent investigations (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11). The accumulation of Ca ++ during respiration has been found to be quite massive, attaining values as high as 3.0/~moles Ca++ per mg mitochondrial protein (2)(3)(4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

“…The accumulation of Ca ++ during respiration has been found to be quite massive, attaining values as high as 3.0/~moles Ca++ per mg mitochondrial protein (2)(3)(4)(5)(6). It is accompanied by uptake of inorganic phosphate from the medium; the molar ratio Ca:P of the accumulated salt is about 1.67, or approximately that of hydroxyapatite (4,5).…”

Section: Introductionmentioning

confidence: 99%

Effect of Active Accumulation of Calcium and Phosphate Ions on the Structure of Rat Liver Mitochondria

Greenawalt

Rossi

Lehninger

1964

The Journal of Cell Biology

357

126

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Rat liver mitochondria allowed to accumulate maximal amounts of Ca ++ and HPO4 = ions from the suspending medium in vitro during respiration have a considerably higher specific gravity than normal mitochondria and may be easily separated from the latter by isopycnic centrifugation in density gradients of sucrose or cesium chloride. When the mitochondria are allowed to accumulate less than maximal amounts of Ca++ and HPO4= from the medium, they have intermediate specific gravities which are roughly proportional to their content of calcium phosphate. Maximally "loaded" mitochondria are relatively homogeneous with respect to specific gravity. Correlated biochemical and electron microscopic studies show that Ca++-loaded mitochondria contain numerous dense granules, of which some 85 per cent are over 500 A in diameter. These granules are electron-opaque not only following fixation and staining with heavy metal reagents, but also following fixation with formaldehyde, demonstrating that the characteristic granules in Ca++-loaded mitochondria have intrinsic electron-opacity. The dense granules are almost always located within the inner compartment of the mitochondria and not in the space between the inner and outer membranes. They are frequently located at or near the cristae and they often show electrontransparent "cores." Such granules appear to be made up of clusters of smaller dense particles, but preliminary x-ray diffraction analysis and electron diffraction studies have revealed no evidence of crystallinity in the deposits. The electron-opaque granules decrease in number when the Ca++-loaded mitochondria are incubated with 2,4-dinitrophenol; simultaneously there is discharge of Ca ++ and phosphate from the mitochondria into the medium.

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Participation of an Intermediate of Oxidative Phosphorylation in Ion Accumulation by Mitochondria

Cited by 110 publications

References 6 publications

Ion Uptake by Soybean Root Tissue Depleted of Calcium by Ethylenediaminetetraacetic Acid

Ion Uptake by Soybean Root Tissue Depleted of Calcium by Ethylenediaminetetraacetic Acid

Utilization of Adenosine Triphosphate in Rat Mast Cells During Histamine Release Induced by the Ionophore A23187

Effect of Active Accumulation of Calcium and Phosphate Ions on the Structure of Rat Liver Mitochondria

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