Participation of CGRP and prostanoids in the sex-linked differences of vascular anandamide effects in mesenteric beds of Sprague-Dawley rats

Peroni, Roxana N.; Orliac, Marı́a Luz; Abramoff, Tamara; Ribeiro, Maria L.C.; Franchi, A.M.; Adler‐Graschinsky, Edda

doi:10.1016/j.ejphar.2006.11.012

Cited by 16 publications

(15 citation statements)

References 26 publications

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“…The presence of this antagonist did not modify the vasoconstrictor response to EFS in female WKY rats, as has also been observed in male rats [5], and this indicates that sensory innervation is not involved in this response in normotension. These results do not concur with previous studies indicating an increased sensory function in females [30,31]. The differences between our data and these studies may be because we used endothelium-denuded arteries, and the vasodilator effect of CGRP is mediated by endothelial NO [32,33] the production of which is greater in females than males [34].…”

Section: Discussioncontrasting

confidence: 82%

Hypertension alters the function of nitrergic and sensory innervation in mesenteric arteries from female rats

Campo

Ferrer

Balfagón

2009

Journal of Hypertension

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Section: Discussioncontrasting

confidence: 82%

Hypertension alters the function of nitrergic and sensory innervation in mesenteric arteries from female rats

Campo

Ferrer

Balfagón

2009

Journal of Hypertension

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“…We hypothesized that hypoxia-induced IUGR would cause changes in vascular function that would affect the mechanisms of endothelium-dependent vasodilation. Since vascular endothelial function is also known to be affected by age (5,20) and sex (32,36), we also investigated whether changes associated with IUGR were age or sex dependent.…”

mentioning

confidence: 99%

Mechanisms of endothelium-dependent vasodilation in male and female, young and aged offspring born growth restricted

Morton

Rueda-Clausen

Davidge

2010

American Journal of Physiology-Regulatory, Integrative and Comp

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Numerous epidemiological studies have shown that cardiovascular dysfunction in adult life may be programmed by compromised growth in utero. Aging is a risk factor for vascular endothelial-dependent dysfunction. After birth, the impact of intrauterine growth restriction (IUGR) on normal aging mechanisms of vascular dysfunction is not known. We hypothesized that IUGR would cause changes in vascular function that would affect the mechanisms of endothelium-dependent vasodilation later in life in an age- or sex-dependent manner. To create an IUGR model, pregnant Sprague-Dawley rats were placed in a hypoxic (12% O(2)) or control (room air, 21% O(2)) environment from days 15 to 21 of the pregnancy, and both male and female offspring were investigated at 4 or 12 mo of age. Endothelial function was assessed in small mesenteric arteries using methacholine (MCh)-induced vasodilation in a wire myograph system. The involvement of nitric oxide (NO), prostaglandins, and endothelium-derived hyperpolarizing factor (EDHF) was assessed using the inhibitors N(omega)-nitro-l-arginine methyl ester hydrochloride, meclofenamate, or a combination of apamin and TRAM-34 (SK(Ca) and IK(Ca) blockers), respectively. EDHF-induced vasodilation was further investigated by using inhibitors of P450 epoxygenases [N-methylsulfonyl-6-(2-propargyloxyphenyl) hexanamide] and gap junctions (18alpha-glycyrrhetinic acid). NO-mediated vasodilation was significantly reduced in aged controls and both young and aged IUGR females. EDHF-mediated vasodilation was maintained in all groups; however, an additional involvement of gap junctions was found in females exposed to hypoxia in utero, which may represent a compensatory mechanism. A change in the mechanisms of vasodilation occurring at an earlier age in IUGR offspring may predispose them to adult cardiovascular diseases.

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“…The experiments were performed according to [6]. Deeply anaesthetized rats were fixed by transcardiac perfusion with PBS containing 4% w/v paraformaldehyde, and mesenteric vascular beds were dissected.…”

Section: Methodsmentioning

confidence: 99%

“…Inhibition of the fatty acid amide hydrolase, enzyme involved in intracellular anandamide degradation, normalizes the cardiovascular function in hypertensive rats without producing adverse metabolic effects [4]. Estrogens are also positive modulators of the anandamide effects at the vascular wall since they stimulate the release of anandamide from human endothelial cells [5] as well as potentiate the anandamide-induced vasorelaxations by increasing the bioavailability of the calcitonin-related peptide (CGRP) in the rat mesenteric vasculature [6]. This potent vasodilator peptide is released, at least in part, as a consequence of the activation of the transient receptor potential vanilloid type 1 (TRPV1) by anandamide [7].…”

Section: Introductionmentioning

confidence: 99%

Phytoestrogens Enhance the Vascular Actions of the Endocannabinoid Anandamide in Mesenteric Beds of Female Rats

Peroni

Abramoff

Neuman

et al. 2012

International Journal of Hypertension

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In rat isolated mesenteric beds that were contracted with NA as an in vitro model of the vascular adrenergic hyperactivity that usually precedes the onset of primary hypertension, the oral administration (3 daily doses) of either 10 mg/kg genistein or 20 mg/kg daidzein potentiated the anandamide-induced reduction of contractility to NA in female but not in male rats. Oral treatment with phytoestrogens also restored the vascular effects of anandamide as well as the mesenteric content of calcitonin gene-related peptide (CGRP) that were reduced after ovariectomy. The enhancement of anandamide effects caused by phytoestrogens was prevented by the concomitant administration of the estrogen receptor antagonist fulvestrant (2.5 mg/kg, s.c., 3 daily doses). It is concluded that, in the vasculature of female rats, phytoestrogens produced an estrogen-receptor-dependent enhancement of the anandamide-vascular actions that involves the modulation of CGRP levels and appears to be relevant whenever an adrenergic hyperactivity occurs.

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Participation of CGRP and prostanoids in the sex-linked differences of vascular anandamide effects in mesenteric beds of Sprague-Dawley rats

Cited by 16 publications

References 26 publications

Hypertension alters the function of nitrergic and sensory innervation in mesenteric arteries from female rats

Hypertension alters the function of nitrergic and sensory innervation in mesenteric arteries from female rats

Mechanisms of endothelium-dependent vasodilation in male and female, young and aged offspring born growth restricted

Phytoestrogens Enhance the Vascular Actions of the Endocannabinoid Anandamide in Mesenteric Beds of Female Rats

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