Participation of covalently linked fatty acryl coenzyme A products in the action of yeast fatty acid synthetase

Localization of three non‐thiol binding sites on polypeptide chain β yeast fatty acid synthetase

“…The binding of malonyl and palmitoyl binding sites on the same polypeptide chain is in good agreement with other observations indicating an identity of malonyl transferase and palmitoyl transferase [12,[14][15][16].…”

The Palmityl Binding Sites of Fatty Acid Synthetase from Yeast

“…Solvent-exchange chromatography on Sephadex G-50 was carried out exactly as described [9]. A column volume of 30 x 6.6 cm was sufficient for the separation of 40-60 nmol of ['4C]palmityl peptides.…”

“…The preparative separation of palmityl peptides and water-soluble peptides was achieved by column chromatography. Solvent-exchange chromatography of palmityl peptides on Sephadex G-50 was performed as described earlier [9]. The radioactive peptides were eluted with the organic phase immediately with the void volume and still were contaminated with small amounts of non-radioactive material.…”

“…Ayling et al [9] have reported on the amino acid composition of the acyl carrier protein in the neighbourhood of the 4'-phosphopantetheine residue. Thus the structure suggested in their paper presumably overlaps the tryptic peptide TZ described in our report.…”

“…Previously it has been shown that by reaction of yeast fatty acid synthetase with [14C]palmityl-CoA the [14C]palmityl residue is transferred to the prosthetic group of the acyl carrier protein, to the condensing enzyme and to the palmityl transferase [9]. In this paper we wish to describe the purification of tryptic and peptic [14C]palmityl peptides from the labelled enzyme.…”