Participation of cyclooxygenase pathway in the vasoconstriction induced by 5-HT in the in situ autoperfused kidney of long-term diabetic rats

5-HT1D receptor inhibits renal sympathetic neurotransmission by nitric oxide pathway in anesthetized rats

“…5-HT showed a slight inhibition the electrically-induced PP increases, while α-methyl-5-HT did not modify them. These results are in agreement with those obtained previously by us [20][21][22]24], where 5-HT 2 receptor activation was involved in vasoconstrictor actions of renal vasculature. 5-HT 2 receptors are generally considered as "sympathoexcitatory" [14,27,28]; however, our research team has demonstrated that the vasoconstrictor effect by 5-HT 2 in renal territory is mediated by the renin- angiotensin system [20].…”

“…pretreatment significantly increased SBP and PP in all cases (see Table 1); i.a. injection of 5-HT or α-methyl-5-HT (0.0125 μg/kg each) increased PP (11.2 ± 1.7 and 16.5 ± 1.4, respectively) that immediately returned to baseline levels [20][21][22]24]. .…”

“…Flow was kept constant throughout the experiments and changes in the PP reflected changes in renal vascular resistance. The flow rate through the renal vascular beds ranged from 2 to 2.9 ml/min [22]. In all experiments, atropine (1 mg/kg) was administered intravenously before the saline infusion in order to block potential cholinergic effects.…”

“…Blood was pumped from the right carotid artery to an aortic pouch from which the left renal artery was the only outlet [19,21,22]. The distal portion of the external circuit was connected to a pressure transducer connected to an e-corder 410 amplifier (Model ED410, Cibertec, Spain) for measurement of the PP.…”

“…The dose of each antagonist was selected after consideration of our previous experience [13,[21][22][23].…”