Participation of endogenous endothelins in delayed eosinophil and neutrophil recruitment in mouse pleurisy

Sampaio, André Luiz Franco; Rae, Giles A.; Henriques, Maria das Graças

doi:10.1007/s000110050577

Cited by 29 publications

(30 citation statements)

References 35 publications

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“…5). These results support the previous finding of significant suppression by BQ-123 of the eosinophil accumulation in BALF in sensitized mice after antigen challenge [7,26,33]. Based on these findings, we speculate that ET-1(1-31) elicits eosinophil recruitment, probably through an ET A receptor, similar to the mechanism of action of ET-1.…”

Section: Discussionsupporting

confidence: 81%

“…In this paper, we first reported that ET-1(1-31) and ET-1 stimulate the generation of eotaxin through a BQ-123-sensitive ET A or ET A -like receptor, resulting in the recruitment of eosinophils to the injected loci. Although it has been reported that the production of IL-5 evoked by an antigen is inhibited by BQ-123 [26], we demonstrated stimulatory effects of ET-1(1-31) and ET-1 on the production of IL-5 in non-immunized mice and their remarkable effects in IL-5 transgenic mice (Figs. 2 and 3).…”

Section: Discussionmentioning

confidence: 80%

“…The current study demonstrates that in allergic inflammation, endogenous ET derivatives participate through the ET A receptor in the accumulation of granulocytes, lymphocytes and eosinophils, and cytokine production [7,26]. In asthmatic patients and animal models of asthma, marked increases in the levels of ET-like immunoreactivity and eosinophil migration in the airway epithelium and bronchoalveolar lavage fluid are evident [10, 27 -29].…”

Section: Discussionmentioning

confidence: 99%

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A novel bioactive 31-amino acid ET-1 peptide stimulates eosinophil recruitment and increases the levels of eotaxin and IL-5

Sharmin¹,

Shiota²,

Murata³

et al. 2002

Inflamm. res.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A novel bioactive 31-amino acid ET-1 peptide stimulates eosinophil recruitment and increases the levels of eotaxin and IL-5

Sharmin¹,

Shiota²,

Murata³

et al. 2002

Inflamm. res.

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“…[12][13][14] The present work provides evidence for several relevant mechanisms underlying this potential benefit. We propose that the beneficial role of ω-3 PUFA on SCD vascular dysfunction might be exerted: (i) directly through the reduction of ET-1 expression, which is involved in adaptive inflammation, expression of adhesion molecules such as VCAM-1, and neutrophil chemotaxis; 46 and (ii) indirectly through reduced inflammation and oxidative stress. Importantly, the ω-3 PUFA supplementation in our study is comparable to that achieved in recent human studies.…”

Section: Discussionmentioning

confidence: 99%

Dietary -3 fatty acids protect against vasculopathy in a transgenic mouse model of sickle cell disease

et al. 2015

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The anemia of sickle cell disease is associated with a severe inflammatory vasculopathy and endothelial dysfunction, which leads to painful and life-threatening clinical complications. Growing evidence supports the anti-inflammatory properties of ω-3 fatty acids in clinical models of endothelial dysfunction. Promising but limited studies show potential therapeutic effects of ω-3 fatty acid supplementation in sickle cell disease. Here, we treated humanized healthy and sickle cell mice for 6 weeks with ω-3 fatty acid diet (fish-oil diet). We found that a ω-3 fatty acid diet: (i) normalizes red cell membrane ω-6/ ω-3 ratio; (ii) reduces neutrophil count; (iii) decreases endothelial activation by targeting endothelin-1 and (iv) improves left ventricular outflow tract dimensions. In a hypoxia-reoxygenation model of acute vaso-occlusive crisis, a ω-3 fatty acid diet reduced systemic and local inflammation and protected against sickle cell-related end-organ injury. Using isolated aortas from sickle cell mice exposed to hypoxia-reoxygenation, we demonstrated a direct impact of a ω-3 fatty acid diet on vascular activation, inflammation, and anti-oxidant systems. Our data provide the rationale for ω-3 dietary supplementation as a therapeutic intervention to reduce vascular dysfunction in sickle cell disease. ABSTRACTmice. 16,17 The animal protocol was approved by the Animal Care and Use Committee of the University of Verona (CIRSAL). Twomonth old animals were fed for 6 weeks with either the standard AIN-93M purified rodent diet (soy-diet with n6/n3 ratio of 8:1 -Dyets Inc., Bethlehem, PA, USA), containing 140 g/kg casein, 1.8 g/kg L-cystine, 100 g/kg sucrose, 465.9 g/kg cornstarch, 155 g/kg dextrose, 40 g/kg soybean oil, 0.8 mg/kg t-butylhydroquinone, 50 g/kg cellulose, 35 g/kg mineral mix, 10 g/kg vitamin mix, and 2.5 g/kg choline bitartrate 18 or the ω-3 FD, an AIN-93M-based purified rodent diet in which all of the calories provided by fat (10%) are replaced by 7.9% from HCO and 2.1% from ω-3 oil (Dyets Inc., Bethlehem, PA, USA). At the end of the 6 weeks of FD supplementation, animals were anesthetized with isofluorane, and whole blood was collected from each mouse via retro-orbital venipuncture by heparinized microcapillaries. Mice were euthanized and organs removed immediately. The organs were divided into two and either frozen immediately in liquid nitrogen or fixed in 10% formalin and embedded in paraffin for histology. Whenever indicated, 6-week old mice were exposed to hypoxia (8% oxygen for 10 h) followed by 3 h of reoxygenation (21% oxygen) (H/R stress) to mimic an acute VOC, as previously described.

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“…Está bem demonstrado que a injeção intratorácica de zimosan e carragenina induziu um intenso extravasamento protéico, bem como a migração de leucócitos, para a cavidade pleural de camundongos (HENRIQUES, et al 1990;SAMPAIO;ERA;HENRIQUES, 2000). , 1996).…”

Section: Atividade Farmacológicaunclassified

Desenvolvimento de microcápsulas contendo a fração volátil de copaíba por 'spray-drying': estudo de estabilidade e avaliação farmacológica

Ramos¹

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AGRADECIMENTOSA oleorresina de copaíba é amplamente utilizada em diferentes regiões do país, especialmente na região amazônica. Sua atividade cicatrizante e antiinflamatória são as mais difundidas na medicina popular. Entretanto, são raras na literatura científica as descrições destas atividades, bem como a indicação da composição responsável pela atividade. Os objetivos deste estudo foram a otimização da obtenção da fração volátil da oleoressina de copaíba, por extração com arraste à vapor, sua microencapsulação em goma arábica por atomização e secagem em spray-dryer, avaliação química, morfológica e farmacológica (atividade antiinflamatória in vivo). A fração volátil representou 14,5% da oleorresina, sendo o β-cariofileno (70%) e o α-humuleno (8,7%) seus constituintes majoritários. A eficiência do processo de microencapsulação da fração volátil em goma arábica foi em média de 95%. Os estudos de estabilidade da fração volátil livre e microcapsulada, nas temperaturas de 25 o C e 40 o C em ambiente de umidade relativa de 50%, mostraram que o β-cariofileno sofreu oxidação, gerando principalmente óxido de cariofileno, e em menor extensão outros compostos oxigenados. A microencapsulação da fração volátil minimizou, porém não impediu a oxidação e a perda de massa. Os ensaios da atividade farmacológica in vivo mostraram que a fração volátil inibiu expressivamente o processo inflamatório agudo, induzido por carragenina ou zimosan, com ED50 de 32mg/Kg. A fração microencapsulada inibiu a resposta inflamatória na mesma magnitude que a fração volátil livre, mostrando que o processo de microencapsulação não alterou a atividade, possibilitando seu uso como forma farmacêutica ou intermediária na preparação de outras. O β-cariofileno inibiu a resposta inflamatória na dose de 32 mg/Kg, na mesma magnitude que a fração volátil, o que nos permite atribuir a este, a atividade antiinflamatória observada.Palavras-chave: fração volátil, oleoressina de copaíba, microencapsulação, β-cariofileno, antiinflamatório. The oilresin of copaiba is extensively used in different regions of Brazil, especially in the Amazon region. Its healing and anti-inflammatory activities are widely known in folk medicine. However, descriptions of these activities and the indication of the compound responsible for these activities are rare in the literature. The objective of the present study was to optimize the derivation of the volatile fraction of the oilresin of copaiba by vapor extraction, its microencapsulation in Arabic gum by atomization and drying with a spray-dryer, and chemical, morphological and pharmacological evaluation (anti-inflammatory activity in vivo). The volatile fraction represented 4.5% of the oilresin, with β-caryophyllene (70%) and α-humulene (8.7%) being its major constituents. The efficiency of the microencapsulation process of the volatile fraction in Arabic gum was on average 95%. The study of stability of the free and microencapsulated volatile fraction at the temperature of 25 o C and 40 o C in the presence of 50% humidity showed ...

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Participation of endogenous endothelins in delayed eosinophil and neutrophil recruitment in mouse pleurisy

Abstract: Endothelins, acting via ETA receptors, play a role in late eosinophil and neutrophil accumulation (24 h), but not in the acute (4 h) neutrophilic response.

Cited by 29 publications

References 35 publications

A novel bioactive 31-amino acid ET-1 peptide stimulates eosinophil recruitment and increases the levels of eotaxin and IL-5

A novel bioactive 31-amino acid ET-1 peptide stimulates eosinophil recruitment and increases the levels of eotaxin and IL-5

Dietary -3 fatty acids protect against vasculopathy in a transgenic mouse model of sickle cell disease

Desenvolvimento de microcápsulas contendo a fração volátil de copaíba por 'spray-drying': estudo de estabilidade e avaliação farmacológica

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