Participation of granulocyte colony-stimulating factor in the growth regulation of leukemia cells from Philadelphia chromosome-positive acute leukemia and blast crisis of chronic myeloid leukemia

Inukai, Takeshi; Sugita, Kanji; Mitsui, Kazuhiro; Iijima, K; Goi, Kumiko; Tezuka, Tohru; Kojika, Satoru; Kagami, Keiko; Mori, Takehiko; Kinoshita, Akitoshi; Suzuki, Takaya; Okazaki-Koyama, T; Nakazawa, Shinpei

doi:10.1038/sj.leu.2401837

Cited by 14 publications

(15 citation statements)

References 43 publications

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“…All cell lines other than NALM1, NALM6, and Jurkat were established in our laboratory and have been described previously. [21][22][23][24] Mononuclear cells separated from cord blood (CB-MNC) were used as a source of hematopoietic stem cells in colony-forming assays. …”

Section: Human Leukemic Cell Lines and Cord Blood Cellsmentioning

confidence: 99%

The JAK2 inhibitor AG490 predominantly abrogates the growth of human B-precursor leukemic cells with 11q23 translocation or Philadelphia chromosome

et al. 2001

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The Janus kinase (JAK) family is one of intracellular protein tyrosine kinases (PTKs) present in hematopoietic and lymphoid cells and has been shown to play a crucial role in a variety of biological responses. It was reported that a human B-precursor leukemic cell line was potently inhibited in its proliferation by one of synthetic PTK inhibitors (tyrphostins), AG490, via anti-JAK2 activity. However, no extensive studies about it have been performed. In the present study, we tested 16 human lymphoid leukemic cell lines (B-precursor, 12; T cell, four) for their sensitivity to AG490 using 3 H-thymidine incorporation and colony formation assays, and found that B-precursor cell lines with 11q23 translocation or Philadelphia chromosome (Ph1) whose JAK2 proved to be constitutively phosphorylated were predominantly sensitive to AG490 at a concentration that has few inhibitory effect on normal hematopoiesis. We first revealed the association of JAK2 with BCR-ABL in Ph1-positive cell lines and with Bruton's tyrosine kinase (BTK) in cell lines with 11q23 translocation by coimmunoprecipitation experiments. Of interest, AG490 markedly down-regulated phosphorylation of JAK2, but rather transiently up-regulated phosphorylation of BCR-ABL and BTK, suggesting direct implication of AG490 in the process of the JAK2 dephosphorylation. These results indicate that AG490 exerts a potent inhibitory activity to B-precursor leukemia with specific chromosomal abnormalities, and a therapeutic approach using AG490 is expected. Leukemia (2001Leukemia ( ) 15, 1758Leukemia ( -1768

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Section: Human Leukemic Cell Lines and Cord Blood Cellsmentioning

confidence: 99%

The JAK2 inhibitor AG490 predominantly abrogates the growth of human B-precursor leukemic cells with 11q23 translocation or Philadelphia chromosome

et al. 2001

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“…28,35 We demonstrated in this study that both primary leukemia cells and established cell lines with 11q23 translocation or Ph1 were considerably responsive to TPO in the 3 H-thymidine uptake assay, the former was more sensitive than the latter. In the experiments using leukemic cell lines with 11q23 translocation or Ph1 whose proliferation was stimulated in the 3 H-thymidine uptake assay, only a weak stimulative effect was observed by addition of TPO alone in the colony formation assay, but a combined addition of GM-CSF and TPO markedly increased the size of colonies.…”

Section: Discussionmentioning

confidence: 64%

“…We have recently shown leukemia cells with 11q23 translocation or Ph1 express G-CSF receptor. 28,35 In this regard, these Bprecursor leukemia cells may express receptors for a variety of cytokines which have been believed to be expressed primarily on non-lymphoid leukemia cells.…”

Section: Discussionmentioning

confidence: 99%

Expression of thrombopoietin receptor and its functional role in human B-precursor leukemia cells with 11q23 translocation or Philadelphia chromosome

et al. 2000

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“…We therefore used 697Bcl2 cells as a negative control for glucocorticoid-induced apoptosis in leukemia 697 cells. All cell lines examined harbored a chromosomal translocation; thus, BV173 (Pegoraro et al 1983), KOPN72bi, KOPN66bi, KOPN57bi (Inukai et al 2000), and OM9;22 (Ohyashiki et al 1993) harbor the t(9;22) translocation (known as BCR-ABL), the t(11;19) translocation (known as MLL-ENL) is harbored byKOCL33, and the t(4;11) translocation (known as MLL-AF4) is harbored by KOCL69 (Yamamoto et al 1994). All cell lines were grown in RPMI-1640 medium supplemented with 10% heatinactivated fetal calf serum, 50 U mL -1 penicillin, and 0.1 mg mL -1 streptomycin.…”

Section: Cell Linesmentioning

confidence: 99%

Glucocorticoid-induced granzyme A expression can be used as a marker of glucocorticoid sensitivity for acute lymphoblastic leukemia therapy

et al. 2007

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Participation of granulocyte colony-stimulating factor in the growth regulation of leukemia cells from Philadelphia chromosome-positive acute leukemia and blast crisis of chronic myeloid leukemia

Cited by 14 publications

References 43 publications

The JAK2 inhibitor AG490 predominantly abrogates the growth of human B-precursor leukemic cells with 11q23 translocation or Philadelphia chromosome

The JAK2 inhibitor AG490 predominantly abrogates the growth of human B-precursor leukemic cells with 11q23 translocation or Philadelphia chromosome

Expression of thrombopoietin receptor and its functional role in human B-precursor leukemia cells with 11q23 translocation or Philadelphia chromosome

Glucocorticoid-induced granzyme A expression can be used as a marker of glucocorticoid sensitivity for acute lymphoblastic leukemia therapy

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