Participation of high-density lipoprotein in vitellogenesis in Japanese eel hepatocytes

Kumagai, Akiko; Hayashi, Seiichi

doi:10.1016/j.cbpc.2004.12.004

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…Studies on the Japanese eel (Anguilla japonica) using pharmacologically high doses (50-75 μg/g BW) of 17MT [35,36] reported oocytes in the advanced yolk vesicle stage. This research appears to be an anomaly as most eel researchers have reported that androgen treatment alone does not induce observable yolk sequestration in eel oocytes, regardless of the potential for some androgens to be aromatized and of the known role of E 2 in stimulating hepatic vitellogenin production [21,22,24,25].…”

Section: Discussionmentioning

confidence: 99%

Effects of estradiol and 11-ketotestosterone pre-treatment on artificial induction of maturation in silver female shortfinned eels (Anguilla australis)

et al. 2020

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Our previous work documented significant advancements in steroid-induced progression of oogenesis, demonstrating that co-treatment of female eels with 11-ketotestosterone (11KT) and estradiol-17β (E 2) successfully induced uptake of vitellogenin by oocytes. Here we evaluate the effects of this steroid co-treatment on subsequent time to ovulation and egg quality in shortfinned eels artificially matured by hypophysation. Co-treatment with 11KT (1 mg) and E 2 (0.2 or 2 mg) significantly reduced time to ovulation and therefore, the amount of pituitary homogenate required, without any detrimental effects on gonadosomatic index, oocyte diameter or the total weight of stripped eggs. E 2 treatment resulted in promising increases in fertilization rates. These indicators suggest that co-treatment with 11KT and E 2 holds promise for future artificial maturation practices in terms of minimising fish handling and stress, and of reducing the need for expensive pituitary preparations.

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Section: Discussionmentioning

confidence: 99%

Effects of estradiol and 11-ketotestosterone pre-treatment on artificial induction of maturation in silver female shortfinned eels (Anguilla australis)

et al. 2020

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“…In the presence of 10 -5 M estradiol-17β, HDL stimulated the induction of vitellogenin synthesis which in the presence of 10 -5 M estradiol-17β and HDL was three times higher than that in the presence of 10 -5 M estradiol-17β alone (Fig. 33) (Kumagai and Hayashi 2005). At concentrations of 10 -6 and 10 -7 M estradiol-17β, vitellogenin induction in the absence of HDL was not detected by the ELISA method.…”

Section: -5c Stimulatory Effect Of Hdl On Vitellogenin Synthesis Anmentioning

confidence: 91%

Studies on Eel Liver Functions Using Perfused Liver and Primary Cultured Hepatocytes

Hayashi¹,

Kumagai²

2008

Aqua-BioSci. Monogr. (ABSM)

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Perfused eel livers, isolated eel hepatocytes, and cultured eel hepatocytes are used to investigate eel liver functions such as gluconeogenesis, glycogen synthesis, and lipoprotein synthesis and methods for preparation are described. A novel phosphoenolpyruvate (PEP) synthesis pathway from pyruvate in gluconeogenesis in eel liver was elucidated and PEP synthesis pathways in eel, rat, and pigeon livers were compared. Glycogen synthesis from pyruvate, lactate, and glucose was investigated by using cultured eel hepatocytes. It was found that 10-6 or 10-7 M glucagon didn't stimulate glycogen degradation in the presence of pyruvate but stimulated glycogen degradation in the presence of lactate. Glycogen synthesis from pyruvate was observed even when 10-6 or 10-7 M glucagon was present. The characteristics of the lipoprotein synthesized and secreted by cultured eel hepatocytes are clarified. Thyroxine and eel serum high-density lipoprotein (HDL) stimulated the lipoprotein synthesis by cultured eel hepatocytes. In the presence of estradiol-17β, eel serum HDL stimulated vitellogenin synthesis in eel hepatocytes. HDL specifically bound to eel hepatocytes and the ligand of HDL receptor in the plasma membrane of eel hepatocytes was identified to be ganglioside GM4 of eel serum HDL.

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“…1999). A study in Japanese eel showed hepatocytes bound specifically to HDL and facilitated the production of vitellogenin (Kumagai and Hayashi 2005). Another study identified the HDL binding protein in the eel liver that plays role in lipid transport with HDL (Katoh et al .…”

Section: Resultsmentioning

confidence: 99%

Changes of the intestinal microbiota along the gut of Japanese Eel ( Anguilla japonica )

Zhu

Wong

Lin

et al. 2021

Lett Appl Microbiol

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Fish intestine contains different types of microbiomes, and bacteria are the dominant microbiota in fishes. Studies have identified various core gut bacteria in fishes. However, little is known about the composition and their relative functions of gut microbial community along the intestine. To explore this, the current study investigated the microbial community distribution along the gut in Anguilla japonica. By 16S rRNA gene sequencing, we profiled the gut microbiota in eel along the three regions (anterior intestine (AI), the middle intestine (MI) and the posterior intestine (PI)). Results suggested that the three regions did not have significant differences on the observed species and diversities. The cluster tree analysis showed that the bacteria community in MI was closer to PI than the AI. The dominant bacteria in AI were the Proteobacteria, in which the majority was graduated replaced by Bacteroidetes along the gut to PI region. Through PICRUSt analysis, shifts in the bacterial community along the gut were found to affect the genetic information processing pathways. Higher levels of translation and transcriptional pathway activities were found in MI and PI than in AI. The dominant bacterial species were different among the regions and contributed to various biological functions along the gut.

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Participation of high-density lipoprotein in vitellogenesis in Japanese eel hepatocytes

Cited by 6 publications

References 25 publications

Effects of estradiol and 11-ketotestosterone pre-treatment on artificial induction of maturation in silver female shortfinned eels (Anguilla australis)

Effects of estradiol and 11-ketotestosterone pre-treatment on artificial induction of maturation in silver female shortfinned eels (Anguilla australis)

Studies on Eel Liver Functions Using Perfused Liver and Primary Cultured Hepatocytes

Changes of the intestinal microbiota along the gut of Japanese Eel ( Anguilla japonica )

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