Participation of Mn(II) in the catalysis of laccase, manganese peroxidase and lignin peroxidase from <i>Phlebia radiata</i>

Lundell, Taina; Hatakka, Annele

doi:10.1016/0014-5793(94)00627-x

Cited by 36 publications

(13 citation statements)

References 32 publications

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“…Pyrolysis–GC/MS analysis demonstrated decrease in the amount of spruce wood phenylpropane units with concomitant increase in the number of smaller fragmented products from these lignin units, suggesting that especially the upregulated lignin peroxidases were actively attacking and converting the predominant non-phenolic structures of the spruce wood coniferous lignin. Lignin peroxidase LiP3 of P. radiata is an efficient oxidizer and degrader of non-phenolic lignin β-O-4 dimeric structures [30, 55]. P. radiata short-MnP3 is active against phenolic lignin compounds [29] and in decomposition of pine wood lignin [28].…”

Section: Discussionmentioning

confidence: 99%

Time-scale dynamics of proteome and transcriptome of the white-rot fungus Phlebia radiata: growth on spruce wood and decay effect on lignocellulose

Kuuskeri

Häkkinen

Laine

et al. 2016

Biotechnol Biofuels

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104

139

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BackgroundThe white-rot Agaricomycetes species Phlebia radiata is an efficient wood-decaying fungus degrading all wood components, including cellulose, hemicellulose, and lignin. We cultivated P. radiata in solid state cultures on spruce wood, and extended the experiment to 6 weeks to gain more knowledge on the time-scale dynamics of protein expression upon growth and wood decay. Total proteome and transcriptome of P. radiata were analyzed by peptide LC–MS/MS and RNA sequencing at specific time points to study the enzymatic machinery on the fungus’ natural growth substrate.ResultsAccording to proteomics analyses, several CAZy oxidoreductase class-II peroxidases with glyoxal and alcohol oxidases were the most abundant proteins produced on wood together with enzymes important for cellulose utilization, such as GH7 and GH6 cellobiohydrolases. Transcriptome additionally displayed expression of multiple AA9 lytic polysaccharide monooxygenases indicative of oxidative cleavage of wood carbohydrate polymers. Large differences were observed for individual protein quantities at specific time points, with a tendency of enhanced production of specific peroxidases on the first 2 weeks of growth on wood. Among the 10 class-II peroxidases, new MnP1-long, characterized MnP2-long and LiP3 were produced in high protein abundances, while LiP2 and LiP1 were upregulated at highest level as transcripts on wood together with the oxidases and one acetyl xylan esterase, implying their necessity as primary enzymes to function against coniferous wood lignin to gain carbohydrate accessibility and fungal growth. Majority of the CAZy encoding transcripts upregulated on spruce wood represented activities against plant cell wall and were identified in the proteome, comprising main activities of white-rot decay.ConclusionsOur data indicate significant changes in carbohydrate-active enzyme expression during the six-week surveillance of P. radiata growing on wood. Response to wood substrate is seen already during the first weeks. The immediate oxidative enzyme action on lignin and wood cell walls is supported by detected lignin substructure sidechain cleavages, release of phenolic units, and visual changes in xylem cell wall ultrastructure. This study contributes to increasing knowledge on fungal genetics and lignocellulose bioconversion pathways, allowing us to head for systems biology, development of biofuel production, and industrial applications on plant biomass utilizing wood-decay fungi.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0608-9) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Time-scale dynamics of proteome and transcriptome of the white-rot fungus Phlebia radiata: growth on spruce wood and decay effect on lignocellulose

Kuuskeri

Häkkinen

Laine

et al. 2016

Biotechnol Biofuels

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104

139

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“…Usually, MnP activity is produced in the form of several isoenzymes [7][8][9][10], a phenomenon also observed in the case of the ligninolytic enzymes lignin peroxidase and laccase [1,2]. The significance of this multiplicity is not well understood, although in Phanerochaete chrysosporium it has been shown that the MnP isoenzymes are differentially regulated in response to nutrient limitation and culture age [11].…”

Section: Introductionmentioning

confidence: 99%

Oxidation reactions catalyzed by manganese peroxidase isoenzymes from Ceriporiopsis subvermispora

Urzúa¹,

Larrondo²,

Lobos³

et al. 1995

FEBS Letters

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“…Phenerochaetae chrysosporium has not been reported to produce laccase, although other selective degraders of wood produces a combination of MnP and laccase like, Pleurotus ostreatus (Giardina et al, 1996), Pleurotus eryngii (Muñoz et al, 1997) and Dichomitus squalens (Périé et al, 1998) and a combination of MnP, LiP and laccase by Phlebia radiata (Lundell and Hatakka, 1994) or only laccase by Pycnoporus cinnabarinus (Eggert et al, 1996b). P. radiata produces at least 3 LiPs, 3 MnPs and one laccase (Moilanen et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Isolation and partial purification of fungal ligninolytic enzymes from the forest soil fungi isolated from Bhadra Wildlife Sanctuary

Banakar

Thippeswamy

2014

Front. Biol.

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Screening was done for the isolation of effective lignin degraders from the forest soil samples, by providing lignin as a carbon source through the enrichment method, which leads to the isolation of 8 effective fungal isolates among 14 isolates. Submerged fermentation was done for the production of ligninolytic enzymes with the effective microorganisms by providing Guiaicol as a carbon source. The assay of laccase, lignin peroxidise activity and specific activity was done after the incubation intervals of 2, 4, 6, 7, 8, 10 and 12 days at 27±2°C under shake culture condition. Partially purified protein content was estimated by using Lowry's method. Pleurotus sp. and Phanerochaetae chrysosporium are more effective at the 2nd and 7th days of incubation for the production of laccase and lignin peroxidases among the effective isolates.

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Participation of Mn(II) in the catalysis of laccase, manganese peroxidase and lignin peroxidase from Phlebia radiata

Cited by 36 publications

References 32 publications

Time-scale dynamics of proteome and transcriptome of the white-rot fungus Phlebia radiata: growth on spruce wood and decay effect on lignocellulose

Time-scale dynamics of proteome and transcriptome of the white-rot fungus Phlebia radiata: growth on spruce wood and decay effect on lignocellulose

Oxidation reactions catalyzed by manganese peroxidase isoenzymes from Ceriporiopsis subvermispora

Isolation and partial purification of fungal ligninolytic enzymes from the forest soil fungi isolated from Bhadra Wildlife Sanctuary

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