Participation of monocyte-macrophages and lymphocytes in the production of a factor that stimulates collagenase and prostaglandin release by rheumatoid synovial cells.

Abstract

A B S T R A C T Cultured mononuclear cells from human peripheral blood produce a soluble factor (MCF) that stimulates collagenase and prostaglandin E2 (PGE2) release by cultured rheumatoid synovial cells up to several hundredfold. These target rheumatoid synovial cells lack conventional macrophage markers. To determine which mononuclear cells are the source of MCF, purified populations of monocyte-macrophages, thymus-derived (T) lymphocytes, and bone-marrowderived (B) lymphocytes were prepared. The monocytemacrophages alone produced levels of MCF that were proportional to cell density but unaffected by phytohemagglutinin or pokeweed mitogen. No detectable collagenase activity was produced by the cultured monocyte-macrophages or lymphocytes. Purified T lymphocytes produced levels of MCF -1-3% those of purified monocyte-macrophages in the presence or absence of the above lectins. Purified T lymphocytes

Dendritic cells and the pathogenesis of rheumatoid arthritis

“…Furthermore, their ability to mature in vitro suggests that SF DC are not merely exhausted ST DC. Finally, although DC maturation factors such as tumor necrosis factor ␣ (TNF-␣), IL-1, and CD40 Ϫ L ϩ T cells are present in SF, co-existing negative regulatory factors, such as transforming growth factor ␤ (TGF-␤) and IL-10, may prevent further functional differentiation of SF DC in vivo [37][38][39]. This is particularly relevant in view of the capacity of IL-10 to signal through cell surface IL-10 receptors expressed by SF monocytes but not DC [40].…”

Effect of a mononuclear cell culture medium on collagen and glycosaminoglycan production by synovial cells in culture

“…After 24 h, the medium was again replaced with medium containing the same additions plus 2.5-5 /ICi/ml L-[4-5-3H]proline (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) Ci/mmol, CEA). At the end of the incubation, media and cells were precipitated separately with 5% trichloroacetic acid.…”

The pivotal role of interleukin-1 in the clinical manifestations of rheumatoid arthritis

“…It has been known for more than 20 yr that cell-cell interactions between synoviocytes, lymphocytes and monocytes lead to the production of large amounts of collagenase [1,2]. It is now accepted that IL-1 and TNFα play an important part in this process and act on endothelial cells, synoviocytes, chondrocytes, or bone-derived cells to produce collagenase, other cytokines (e.g.…”