Participation of Nitrate Sensor NRT1.1 in the Control of Cytokinin Level and Root Elongation under Normal Conditions and Nitrogen Deficit

Korobova, A.V.; Akhiyarova, G.R.; Fedyaev, V.V.; Фархутдинов, Р.Г.; Veselov, S. Yu.; Kudoyarova, G. R.

doi:10.3103/s0096392519040072

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…After solvent evaporation, the dry residue was dissolved in 0.02 mL of 80% ethanol, and zeatin metabolites were separated using thin-layer chromatography [ 45 ]. Extraction of ABA and IAA from aliquots of aqueous residues was performed with the diethyl ether, according to the modified method, as described by Kudoyarova et al [ 46 ]. Hormones were immunoassayed using the corresponding specific antibodies.…”

Section: Methodsmentioning

confidence: 99%

PGP-Bacterium Pseudomonas protegens Improves Bread Wheat Growth and Mitigates Herbicide and Drought Stress

Бакаева

Chetverikov

Timergalin

et al. 2022

Plants

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The reaction of plants to simultaneous stress action and treatment with biological stimulants still remains poorly studied. Laboratory and field experiments have been conducted to study the growth and yield of bread wheat (Triticum aestivum L.) of the variety Ekada 113; stress markers and quantitative ratios of phytohormones in plants under insufficient soil moisture; the effects of spraying with herbicide containing 2,4-D and dicamba and growth-stimulating bacterium Pseudomonas protegens DA1.2; and combinations of these factors. Under water shortage conditions, spraying plants with Chistalan reduced their growth compared to non-sprayed plants, which was associated with inhibition of root growth and a decrease in the content of endogenous auxins in the plants. Under conditions of combined stress, the treatment of plants with the strain P. protegens DA1.2 increased the IAA/ABA ratio and prevented inhibition of root growth by auxin-like herbicide, ensuring water absorption by the roots as well as increased transpiration. As a result, the content of malondialdehyde oxidative stress marker was reduced. Bacterization improved the water balance of wheat plants under arid field conditions. The addition of bacterium P. protegens DA1.2 to the herbicide Chistalan increased relative water content in wheat leaves by 11% compared to plants treated with herbicide alone. Application of the bacterial strain P. protegens DA1.2 increased the amount of harvested grain from 2.0–2.2 t/ha to 3.2–3.6 t/ha. Thus, auxin-like herbicide Chistalan and auxin-producing bacterium P. protegens DA1.2 may affect the balance of phytohormones in different ways. This could be the potential reason for the improvement in wheat plants’ growth during dry periods when the bacterium P. protegens DA1.2 is included in mixtures for weed control.

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Section: Methodsmentioning

confidence: 99%

PGP-Bacterium Pseudomonas protegens Improves Bread Wheat Growth and Mitigates Herbicide and Drought Stress

Бакаева

Chetverikov

Timergalin

et al. 2022

Plants

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“…These results suggested that NRT1.1 combines the functions of a sensor and a nitrate carrier, which allows it to be called a transceptor. Cytokinin content decreased in the roots of wild-type Arabidopsis in response to nitrate starvation, whereas the roots of the mutant did not respond to the treatment [ 29 ]. The results suggested the involvement of the NRT1.1 transceptor in the control of cytokinin concentration in accordance with nitrate levels.…”

Section: Long-distance Signaling and Transport Of Cytokininsmentioning

confidence: 99%

The Long-Distance Transport of Some Plant Hormones and Possible Involvement of Lipid-Binding and Transfer Proteins in Hormonal Transport

Akhiyarova,

Finkina,

Zhang

et al. 2024

Cells

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Adaptation to changes in the environment depends, in part, on signaling between plant organs to integrate adaptive response at the level of the whole organism. Changes in the delivery of hormones from one organ to another through the vascular system strongly suggest that hormone transport is involved in the transmission of signals over long distances. However, there is evidence that, alternatively, systemic responses may be brought about by other kinds of signals (e.g., hydraulic or electrical) capable of inducing changes in hormone metabolism in distant organs. Long-distance transport of hormones is therefore a matter of debate. This review summarizes arguments for and against the involvement of the long-distance transport of cytokinins in signaling mineral nutrient availability from roots to the shoot. It also assesses the evidence for the role of abscisic acid (ABA) and jasmonates in long-distance signaling of water deficiency and the possibility that Lipid-Binding and Transfer Proteins (LBTPs) facilitate the long-distance transport of hormones. It is assumed that proteins of this type raise the solubility of hydrophobic substances such as ABA and jasmonates in hydrophilic spaces, thereby enabling their movement in solution throughout the plant. This review collates evidence that LBTPs bind to cytokinins, ABA, and jasmonates and that cytokinins, ABA, and LBTPs are present in xylem and phloem sap and co-localize at sites of loading into vascular tissues and at sites of unloading from the phloem. The available evidence indicates a functional interaction between LBTPs and these hormones.

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“…These responses depended on the function of nitrate sensors (NRT1), called transceptors, due to their dual function as nitrate transporters and receptors [8]. This conclusion was based on experiments with the mutants characterized by impaired NRT1 function and absence of either changes in expression of IPT genes [9] or root cytokinin content [10] under nitrate shortage.…”

Section: Introductionmentioning

confidence: 99%

“…Since cytokinins can inhibit root elongation [15], a decrease in their content was considered as the cause of accelerated root growth in response to deficiencies in mineral nutrients [16]. This suggestion was supported by the absence of root growth response in the nrt1-1 mutant, in which nitrate starvation failed to change root cytokinin content [10].…”

Section: Introductionmentioning

confidence: 99%

Association of Barley Root Elongation with ABA-Dependent Transport of Cytokinins from Roots and Shoots under Supra-Optimal Concentrations of Nitrates and Phosphates

Vysotskaya

Timergalina

Akhiyarova

et al. 2021

Cells

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Changes in root elongation are important for the acquisition of mineral nutrients by plants. Plant hormones, cytokinins, and abscisic acid (ABA) and their interaction are important for the control of root elongation under changes in the availability of ions. However, their role in growth responses to supra-optimal concentrations of nitrates and phosphates has not been sufficiently studied and was addressed in the present research. Effects of supra-optimal concentrations of these ions on root elongation and distribution of cytokinins between roots and shoots were studied in ABA-deficient barley mutant Az34 and its parental variety, Steptoe. Cytokinin concentration in the cells of the growing root tips was analyzed with the help of an immunohistochemical technique. Increased concentrations of nitrates and phosphates led to the accumulation of ABA and cytokinins in the root tips, accompanied by a decline in shoot cytokinin content and inhibition of root elongation in Steptoe. Neither of the effects were detected in Az34, suggesting the importance of the ability of plants to accumulate ABA for the control of these responses. Since cytokinins are known to inhibit root elongation, the effect of supra-optimal concentration of nitrates and phosphates on root growth is likely to be due to the accumulation of cytokinins brought about by ABA-induced inhibition of cytokinin transport from roots to shoots.

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Participation of Nitrate Sensor NRT1.1 in the Control of Cytokinin Level and Root Elongation under Normal Conditions and Nitrogen Deficit

Cited by 8 publications

References 21 publications

PGP-Bacterium Pseudomonas protegens Improves Bread Wheat Growth and Mitigates Herbicide and Drought Stress

PGP-Bacterium Pseudomonas protegens Improves Bread Wheat Growth and Mitigates Herbicide and Drought Stress

The Long-Distance Transport of Some Plant Hormones and Possible Involvement of Lipid-Binding and Transfer Proteins in Hormonal Transport

Association of Barley Root Elongation with ABA-Dependent Transport of Cytokinins from Roots and Shoots under Supra-Optimal Concentrations of Nitrates and Phosphates

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