Participation of stem cells from human cord blood in skeletal muscle regeneration of SCID mice

Brzóska, Edyta; Grabowska, Iwona; Hoser, Grazyna; Stremińska, Władysława; Wasilewska, Danuta; Machaj, Eugeniusz K; Pojda, Zygmunt; Moraczewski, Jerzy; Kawiak, Jerzy

doi:10.1016/j.exphem.2006.05.009

Cited by 35 publications

(36 citation statements)

References 33 publications

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“…It is well known that CB-derived cells contribute to skeletal muscle (Gang et al, 2004;Brzoska et al, 2006), liver (Kakinuma et al, 2003;Newsome et al, 2003;Di Campli et al, 2004), neural tissue (Buzanska et al, 2002), and myocardium regeneration (Henning et al, 2004;Ma et al, 2005), and more importantly, recent multiorgan engraftment and differentiation has been achieved in goats after transplantation of human CB CD34 ϩ lin Ϫ cells (Zeng et al, 2006a). Furthermore, several groups of investigators described the presence of Oct-4 ϩ , Nanog ϩ , and SSEA-3/4 ϩ stem cells in human CB and umbilical cord matrix (Carlin et al, 2006).…”

Section: Oct-4 ؉ Stem Cells In Cord Bloodmentioning

confidence: 99%

Bone marrow‐derived very small embryonic‐like stem cells: Their developmental origin and biological significance

Kucia

Wan

Ratajczak

2007

Developmental Dynamics

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Data from our and other laboratories provide evidence that bone marrow (BM) contains a population of stem cells that expresses early developmental markers such as (1) stage-specific embryonic antigen (SSEA) and (2) transcription factors Oct-4 and Nanog. These are the markers characteristic for embryonic stem cells, epiblast stem cells, and primordial germ cells (PGC). The presence of these stem cells in adult BM supports the concept that this organ contains some population of pluripotent stem cells that is deposited in embryogenesis during early gastrulation. We hypothesize that these cells could be direct descendants of the germ lineage that, to pass genes on to the next generations, has to create soma and, thus, becomes a "mother lineage" for all somatic cell lineages present in the adult body. Germ potential is established after conception in totipotent zygotes and retained in blastomeres of morula, cells from the inner cell mass of blastocyst, epiblast, and population of PGC. We will present a concept that SSEA ؉ Oct-4 ؉ Nanog ؉ cells identified in BM could be descendants of epiblast cells as well as some rare migrating astray PGC. Developmental Dynamics 236:3309 -3320, 2007.

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Section: Oct-4 ؉ Stem Cells In Cord Bloodmentioning

confidence: 99%

Bone marrow‐derived very small embryonic‐like stem cells: Their developmental origin and biological significance

Kucia

Wan

Ratajczak

2007

Developmental Dynamics

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“…fetal, slow, fast IIa, fast IIb and fast IId/x, were used as described previously [29]. Primers and conditions for housekeeping gene glyceraldehyde 3-phosphate (GAPDH) were used as described previously [17]. Ten microliters of each reaction solution was supplemented with 2 µl of Blue-Juice Gel Loading Buffer (Invitrogen Ltd., Paisley, UK) and reaction products were separated by electrophoresis through 2% agarose gel containing ethidium bromide.…”

Section: Methodsmentioning

confidence: 99%

“…Our previous analyses of crush-injured muscles showed that subsequent stages of regeneration can be characterized by specific morphological changes [17]. Just after injury, an inflammatory response takes place, followed by phagocytosis of damaged myofibers and the activation of satellite cells.…”

Section: Muscle Weight and Morphologymentioning

confidence: 99%

“…Just after injury, an inflammatory response takes place, followed by phagocytosis of damaged myofibers and the activation of satellite cells. Next, satellite cells start to proliferate, and fuse into myotubes that finally differentiate into myofibers [17,18]. In the current study, histological analyses were performed on intact ( Figure 1B) and regenerating muscles on the 1 st , 4 th , 6 th , and 14 th days after mechanical or CTX injury ( Figure 1C).…”

Section: Muscle Weight and Morphologymentioning

confidence: 99%

“…Moreover, only a few papers have included a detailed, side by side, comparison of the muscle regeneration induced using different methods, and these publications do not cover all the molecular changes occurring within the muscles [13][14][15]. To fill this gap in knowledge, we aimed to carefully compare the dynamics of regeneration induced by two types of commonly used injury methods: crushing [11,[16][17][18][19][20] and CTX injection [7,[21][22][23][24][25][26]. Crushing, accompanied by denervation, causes muscle degeneration as a result of mechanical devastation of myofibers [11].…”

Section: Introductionmentioning

confidence: 99%

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Mouse gastrocnemius muscle regeneration after mechanical or cardiotoxin injury

Czerwińska

Stremińska

Ciemerych

et al. 2012

Folia Histochem Cytobiol.

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Abstract:The goal of our study was to compare the skeletal muscle regeneration induced by two types of injury: either crushing, that causes muscle degeneration as a result of mechanical devastation of myofibers, or the injection of a cardiotoxin that is a myotoxic agent causing myolysis of myofibers leading to muscle degeneration. Regenerating muscles were analyzed at selected intervals, until the 14 th day following the injury. We analyzed their weight and morphology. We also studied the expression of different myosin heavy chain isoforms as a molecular marker of the regeneration progress. Histological analysis revealed that inflammatory response and myotube formation in crushed muscles was delayed compared to cardiotoxin-injected ones. Moreover, the expression of myosin heavy chain isoforms was observed earlier in cardiotoxin-injured versus crushed muscles. We conclude that the dynamics of skeletal muscle regeneration depends on the method of injury.

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Stromal derived factor‐1 and granulocyte‐colony stimulating factor treatment improves regeneration of Pax7−/− mice skeletal muscles

Kowalski

Archacki

Archacka

et al. 2015

J cachexia sarcopenia muscle

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BackgroundThe skeletal muscle has the ability to regenerate after injury. This process is mediated mainly by the muscle specific stem cells, that is, satellite cells. In case of extensive damage or under pathological conditions, such as muscular dystrophy, the process of muscle reconstruction does not occur properly. The aim of our study was to test whether mobilized stem cells, other than satellite cells, could participate in skeletal muscle reconstruction.MethodsExperiments were performed on wild‐type mice and mice lacking the functional Pax7 gene, that is, characterized by the very limited satellite cell population. Gastrocnemius mice muscles were injured by cardiotoxin injection, and then the animals were treated by stromal derived factor‐1 (Sdf‐1) with or without granulocyte‐colony stimulating factor (G‐CSF) for 4 days. The muscles were subjected to thorough assessment of the tissue regeneration process using histological and in vitro methods, as well as evaluation of myogenic factors' expression at the transcript and protein levels.ResultsStromal derived factor‐1 alone and Sdf‐1 in combination with G‐CSF significantly improved the regeneration of Pax7−/− skeletal muscles. The Sdf‐1 and G‐CSF treatment caused an increase in the number of mononucleated cells associated with muscle fibres. Further analysis showed that Sdf‐1 and G‐CSF treatment led to the rise in the number of CD34+ and Cxcr4+ cells and expression of Cxcr7.ConclusionsStromal derived factor‐1 and G‐CSF stimulated regeneration of the skeletal muscles deficient in satellite cells. We suggest that mobilized CD34+, Cxcr4+, and Cxcr7+ cells can efficiently participate in the skeletal muscle reconstruction and compensate for the lack of satellite cells.

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Participation of stem cells from human cord blood in skeletal muscle regeneration of SCID mice

Cited by 35 publications

References 33 publications

Bone marrow‐derived very small embryonic‐like stem cells: Their developmental origin and biological significance

Bone marrow‐derived very small embryonic‐like stem cells: Their developmental origin and biological significance

Mouse gastrocnemius muscle regeneration after mechanical or cardiotoxin injury

Stromal derived factor‐1 and granulocyte‐colony stimulating factor treatment improves regeneration of Pax7−/− mice skeletal muscles

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