Participation of two human cytomegalovirus immediate early gene regions in transcriptional activation of adenovirus promoters

Tevethia, Mary J.; Spector, David J.; Leisure, K.M.; Stinski, Mark F.

doi:10.1016/0042-6822(87)90119-x

Cited by 101 publications

(78 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However expression of this plasmid was augmented by IE1 sequences, suggesting that IE2 might exert its maximum stimulatory effect only in combination with IE1. These results were confirmed by investigating the ability of different IE genes of HCMV to complement mutants of adenovirus E 1 a and to stimulate expression from the early E2 promoter of adenovirus (Tevethia et al, 1987). It was observed that the stimulatory effect of both IE region 1 and 2 together is about 10-fold higher than that of IE2 alone.…”

Section: Functional Aspects Of Le Gene Productssupporting

confidence: 65%

“…It was observed that the stimulatory effect of both IE region 1 and 2 together is about 10-fold higher than that of IE2 alone. In contrast to Tevethia et al (1987) and Hermiston et al (1987), Pizzorno et al (1988 reported the absence of a cooperative effect from IE 1 and IE2. Furthermore they attributed both the stimulatory and the negative autoregulatory effect of the IE genes to a protein encoded by IE2 sequences.…”

Section: Functional Aspects Of Le Gene Productsmentioning

confidence: 77%

See 1 more Smart Citation

Human Cytomegalovirus: Recent Aspects from Molecular Biology

Mach

Stamminger

Jahn

1989

Journal of General Virology

View full text Add to dashboard Cite

Section: Functional Aspects Of Le Gene Productssupporting

confidence: 65%

Section: Functional Aspects Of Le Gene Productsmentioning

confidence: 77%

Human Cytomegalovirus: Recent Aspects from Molecular Biology

Mach

Stamminger

Jahn

1989

Journal of General Virology

View full text Add to dashboard Cite

“…The IE genes of CMV autoregulate their own expression, as well as the expression of both the early and late genes ( 12). The IE genes have also been shown to modulate the expression of other, heterologous, viral genes ( 13), including those ofthe human immunodeficiency virus ( 14) and adenovirus ( 15,16). Previous studies from our laboratory have demonstrated that the CMV IE gene products can upregulate cell-derived genes, including IL-I ( 17, 18) and IL-2, and IL-2R ( 19).…”

Section: Introductionmentioning

confidence: 99%

The immediate early genes of human cytomegalovirus upregulate tumor necrosis factor-alpha gene expression.

Geist¹,

Monick²,

Stinski³

et al. 1994

J. Clin. Invest.

View full text Add to dashboard Cite

Cytomegalovirus (CMV) is an important cause of disease in the immunocompromised patient and CMV infection is associated with predominantly mononuclear inflammatory response. Since products of the CMV immediate early (IE) gene region are potent trans-activators, we used the monocyte cell line THP-1 and a transient transfection assay to determine if these viral proteins upregulate expression of the TNF gene. The IE genes of CMV upregulated TNF gene activity as judged by increases in promoter activity, steady state mRNA, and protein production. The presence or absence of the 3' untranslated region of the TNF gene did not affect gene expression induced by the IE gene products. These studies suggest that activation of TNF gene expression by the CMV IE gene products may, in part, account for the inflammatory response associated with CMV infections. (J. Clin. Invest. 1994. 93:474-478.)

show abstract

“…This 72-kDa phosphoprotein is abundantly expressed immediately after infection (Plachter et al, 1996). In transient transfection assays, IE1 has been shown to be involved in the autoregulation of its own promoter and also in the transactivation of viral and cellular promoters, alone or in concert with other viral or cellular proteins (Stenberg and Stinski, 1985;Davis et al, 1987;Tevethia et al, 1987;Cherrington and Mocarski, 1989;Sambucetti et al, 1989;Stenberg et al, 1989;Iwamoto et al, 1990;Hagemeier et al, 1992;Monick et al, 1992;Michelson et al, 1994;Margolis et al, 1995;Yurochko et al, 1995;Kim et al, 1999;Murayama et al, 2000). It was also reported that the IE1 gene product could cooperate with another IE gene product, IE2, and adenoviral E1A to transform primary baby rat kidney (BRK) cells (Shen et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Downregulation of GFAP, TSP‐1, and p53 in human glioblastoma cell line, U373MG, by IE1 protein from human cytomegalovirus

Lee

Jeon

Kim

et al. 2005

Glia

View full text Add to dashboard Cite

Human cytomegalovirus (HCMV) is a member of the b-herpesvirus family, which has tropism for glial cells. It was recently reported that HCMV might play important roles in the pathogenesis of malignant glioma. In this study, we investigated the effects of the HCMV IE1 protein on the gene expression profile in the human glioblastoma cell line, U373MG by employing cDNA microarray technology. Using DNA chips containing approximately 1,000 human cDNAs, RNA samples from U373MG cells stably expressing IE1 were compared with those from the control cells lacking IE1 cDNA. Fluorescence intensities of 13 genes were significantly decreased in IE1-expressing cells, while one gene was found to be upregulated. Among these 14 genes, we chose to work further on glial fibrillary acidic protein (GFAP), thrombospondin-1 (TSP-1), and p53, because of their previously known involvement in tumorigenesis. The mRNA levels of all these genes were found to be decreased in IE1-expressing glioblastoma cells by real-time quantitative reverse transcriptionpolymerase chain reaction (RT-PCR) as well as Northern blot analysis. The decreased expression of these genes was also observed at protein levels as measured by immunocytochemistry or fluorescence-activated cell sorting (FACS) analysis. Our data strongly suggested that HCMV IE1 could modulate the expression of cellular genes that might play important roles in the pathogenesis of glial tumors. '

show abstract

Participation of two human cytomegalovirus immediate early gene regions in transcriptional activation of adenovirus promoters

Cited by 101 publications

References 57 publications

Human Cytomegalovirus: Recent Aspects from Molecular Biology

Human Cytomegalovirus: Recent Aspects from Molecular Biology

The immediate early genes of human cytomegalovirus upregulate tumor necrosis factor-alpha gene expression.

Downregulation of GFAP, TSP‐1, and p53 in human glioblastoma cell line, U373MG, by IE1 protein from human cytomegalovirus

Contact Info

Product

Resources

About