Airflow and ventilation are particularly important in healthcare rooms for controlling thermohygrometric conditions, providing anaesthetic gas removal, diluting airborne bacterial contamination and minimizing bacteria transfer airborne. An actual hospitalization room was the investigate case study. Transient simulations with computational fluid dynamics (CFD), based on the finite element method (FEM) were performed to investigate the efficiency of the existing heating, ventilation and air-conditioning (HVAC) plant with a variable air volume (VAV) primary air system. Solid modelling of the room, taking into account thermo-physical properties of building materials, architectural features (e.g., window and wall orientation) and furnishing (e.g., beds, tables and lamps) arrangement of the room, inlet turbulence high induction air diffuser, the return air diffusers and two patients lying on two parallel beds was carried out. Multiphysics modelling was used: a thermo-fluidynamic model (convection-conduction and incompressible Navier-Stokes) was combined with a convectiondiffusion model. Three 3D models were elaborated considering different conditions/events of the patients (i.e., the first was considered coughing and/or the second breathing). A particle tracing and diffusion model, connected to cough events, was developed to simulate the dispersal of bacteria-carrying droplets in the isolation room equipped with the existing ventilation system. An analysis of the region of droplet fallout and the dilution time of bacteria diffusion of coughed gas in the isolation room was performed. The analysis of transient simulation results concerning particle path and distance, and then particle tracing combined with their concentration, provided evidence of the formation of zones that should be checked by microclimatic and contaminant control. The present study highlights the fact that the CFD-FEM application is useful for understanding the efficiency, adequacy and reliability of the ventilation system, but also provides important suggestions for controlling air quality, patients' comfort and energy consumption in a hospital.