Particle size affects the cellular response in macrophages

Yue, Hua; Wei, Wei; Yue, Zhanguo; Lv, Piping; Wang, Lianyan; Ma, Guanghui; Su, Zhiguo

doi:10.1016/j.ejps.2010.09.006

Cited by 161 publications

(88 citation statements)

References 38 publications

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“…Large and insoluble particulate antigens are phagocytosed very efficiently and produce strong responses by themselves. [7][8][9] This is the case for microorganisms such as bacteria and viruses. [10,11] Conversely, small and soluble antigens, such as proteins and peptides, are poorly internalized and thus weakly immunogenic; therefore, an adjuvant is needed to intensify the immune response.…”

Section: Introductionmentioning

confidence: 99%

Carbon nanotube-protein carriers enhance size-dependent self-adjuvant antibody response to haptens

Parra

Abad‐Somovilla

Mercader

et al. 2013

Journal of Controlled Release

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Carbon nanotubes (CNTs) are nanomaterials with interesting emerging applications. Their 25 properties makes CNTs excellent candidates for use as new nanovehicles in drug delivery, immunization and diagnostics. In the current study, we assessed the immune-response-amplifying properties of CNTs to haptens by using azoxystrobin, the first developed strobilurin fungicide, as a model analyte. An azoxystrobin derivative bearing a carboxylated spacer arm (hapten AZc6) was covalently coupled to bovine serum albumin (BSA), and the resulting BSA-AZc6 conjugate was 30 covalently linked to four functionalized CNTs of different shapes and sizes, varying in diameter and length. These four types of CNT-based constructs were obtained using efficient, fast, and easy functionalization procedures based on microwave-assisted chemistry. New Zealand rabbits and BALB/c mice were immunized with BSA-AZc6 alone and with the four CNT-BSA-AZc6 constructs, both with and without Freund's adjuvant. The IgG-type antibody responses were assessed 35 in terms of the titer and affinity, paying special attention to the relationship between the immune response and the size and shape of the employed CNTs. Immunization with CNT-BSA-AZc6 resulted in enhanced titers and excellent affinities for azoxystrobin. More important, remarkable IgG responses were obtained even in the absence of an adjuvant, thus proving the self-adjuvanting capability of CNTs. Immunogens were able to produce strong anti-azoxystrobin immune responses 40 in rabbits even when administered at a BSA-AZc6 conjugate dose as low as 0.05 g. The short and thick CNT-BSA-AZc6 construct produced the best antibody response under all tested conditions.

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Section: Introductionmentioning

confidence: 99%

Carbon nanotube-protein carriers enhance size-dependent self-adjuvant antibody response to haptens

Parra

Abad‐Somovilla

Mercader

et al. 2013

Journal of Controlled Release

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“…It is evident that the smaller sized liposomes obtained using the REV and PD methods exhibited greater inhibition of TNF-α compared with those of the larger sized liposomes obtained by use of the TF method. This may be associated with the more efficient liposome-cell membrane interactions of smaller particles compared with those of larger particles (20,21).…”

Section: Resultsmentioning

confidence: 99%

Anti-inflammatory activity of liposomes of Asparagus racemosus root extracts prepared by various methods

Plangsombat

Rungsardthong

Kongkaneramit

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. Asparagus racemosus root extracts (AR) have been reported to possess a variety of pharmacological properties. The aim of the present study was to develop liposomes of AR and to assess their physicochemical characteristics and anti-inflammatory activity in the monocytic leukemia cell line THP-1. Liposomes containing various ratios of AR to lipid and a phosphatidylcholine to cholesterol molar ratio of 7:3 were prepared by thin-film hydration (TF), reverse-phase evaporation (REV) and polyol dilution (PD). The results showed that AR liposomes prepared by TF had a multilamellar structure and a large size, whereas those prepared by REV and PD were oligolamellar in structure, and of a smaller size. The particle sizes and zeta potentials of the liposomes ranged from 196.5 to 456.6 nm and from -4.34 to -18.94 mV, respectively. The AR to lipid ratio was shown to have no significant influence on particle size, while the zeta potential generally increased with increasing AR to lipid ratio. The highest entrapment efficiency values were detected in liposomes with an AR to lipid ratio of 1:5, and for liposomes prepared by TF, REV and PD methods, the entrapment efficiencies were 55.71±2.04, 56.21±3.59 and 67.68±1.37%, respectively. AR was found to exert no toxicity on THP-1 cells. The maximum anti-inflammatory activities of AR and AR liposomes, evaluated in terms of the percentage inhibition of tumor necrosis factor-α in THP-1 cells, were ~52% at a concentration of 1 µg/ml. It can be concluded from the present study that AR liposomes have the potential to be used a formulation for topical and/or transdermal drug delivery to provide anti-inflammatory activity.

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“…Freeze-drying in the presence of such high concentration of glucose may favor the reaction of remnant BAC groups with cryoprotectant molecules acting as bridges among neighbor NPs. Overall, NPs showed a suitable size for tissue targeting and cell internalization [51][52][53].…”

Section: Preparation Of In Situ Cross-linked Gsh-responsive Nanopartimentioning

confidence: 98%

Glucose cryoprotectant affects glutathione-responsive antitumor drug release from polysaccharide nanoparticles

Curcio

Blanco-Fernandez

Costoya

et al. 2015

European Journal of Pharmaceutics and Biopharmaceutics

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Particle size affects the cellular response in macrophages

Cited by 161 publications

References 38 publications

Carbon nanotube-protein carriers enhance size-dependent self-adjuvant antibody response to haptens

Carbon nanotube-protein carriers enhance size-dependent self-adjuvant antibody response to haptens

Anti-inflammatory activity of liposomes of Asparagus racemosus root extracts prepared by various methods

Glucose cryoprotectant affects glutathione-responsive antitumor drug release from polysaccharide nanoparticles

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