Abstract:Suspension cultures are widely used for cell expansion in regenerative medicine and production. Shear stress caused by the flow of medium in cultures causes cell death and delayed growth; thus, the shear stress on the cell surface must be minimized for successful suspension culture. We established a particle tracking system for estimating the shear stress on induced pluripotent stem cell aggregates during shaking culture and utilized it to optimize shaking conditions (orbital and reciprocating shaking). When t… Show more
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