Particles formed by enzymatic silver deposition for DNA detection by means of SERS

Abstract

Traditional SERS active substrates are roughened metal electrodes, metal layers or metal colloids. Within this contribution nanoparticles produced by enzymatic silver deposition are utilized. These nanoparticles grow directly on the modified oligonucleotides and only in spatially defined areas on the chip surface. The applied biotin coupling system requires only a standard modification of the oligonucleotide, which is simple, stable and cost-effective in comparison to the usually used thiol modification. Furthermore they offer several advantages for SERS detection. The nanoparticles are characterized and their potential for their use as SERS active substrate and the potential for sequence specific DNA analysis is estimated. '''

Fabrication of SERS-Active Patterned Gold Nanoparticle Films by Electron Irradiation and Postpyrolysis

“…While considerable effort for SERS-active substrates has been directed toward the size- and shape-controlled synthesis of noble metal NP arrays, fabrication of a position-controlled assembly or patterning of metal NPs on a solid substrate is still challenging. However, such patterned noble metal NPs are indispensable for the applications to laboratory-on-a-chip devices that allow to detect different molecules simultaneously using one substrate. , Among a few fabrication approaches for SERS-active patterned metal NPs, microcontact printing, , chemical interactions with surface functionalization, , and direct electron beam lithography , are widely used. However, microcontact printing method is not always successful because of the hydrophobic nature of polydimethylsiloxane .…”