2024
DOI: 10.3390/dairy5010013
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Particles in Raw Sheep Milk Can Modulate the Inflammatory Response in THP-1, a Human Monocyte Cell Line, In Vitro

Bigboy Simbi,
Ryan C. Pink,
Louise Whatford
et al.

Abstract: Background: The UK dairy sheep industry is relatively small but growing, particularly for cheese and yogurt products. Anecdotally, sheep milk (SM) may be better tolerated by humans than cows’ milk and could have environmental as well as health benefits. All milk contains sub-micron particles called extracellular vesicles (EVs) which are mainly derived from the mammary epithelium. Physiologically, milk-derived EVs are thought to aid in the development of infant immunity and the microbiome, but may also have hea… Show more

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“…DM and WM were photographically assessed, and pH measured, to confirm that they were of different species ( Supplemental data ). Following the modification of our laboratory’s well-established ultracentrifugation protocol [ 25 ], we isolated EVs from both WM and DM. As shown in Figure 1 , we were able to separate two populations: a population of LEVs which could be identified by the binding of annexin V to the surface and detection via flow cytometry ( Figure 1 C,F) and a population of sEVs which had a typical peak between 136 nm (WM sEVs) and 149 nm (DM sEVs; Figure 1 D,G) per nanotracker analysis (NTA).…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…DM and WM were photographically assessed, and pH measured, to confirm that they were of different species ( Supplemental data ). Following the modification of our laboratory’s well-established ultracentrifugation protocol [ 25 ], we isolated EVs from both WM and DM. As shown in Figure 1 , we were able to separate two populations: a population of LEVs which could be identified by the binding of annexin V to the surface and detection via flow cytometry ( Figure 1 C,F) and a population of sEVs which had a typical peak between 136 nm (WM sEVs) and 149 nm (DM sEVs; Figure 1 D,G) per nanotracker analysis (NTA).…”
Section: Resultsmentioning
confidence: 99%
“…As previously described in our laboratory [ 25 , 39 ], THP-1 monocytes were pelleted, washed, counted, and then incubated with 5 μM of dihydrorhodamine-1,2,3 (DHR) for 10 min in the dark. They were then seeded onto 96-well plates at 10,000 cells per well.…”
Section: Methodsmentioning
confidence: 99%