Particles of liquid–crystalline dispersions formed by (nucleic acid–rare earth element) complexes as a potential platform for neutron capture therapy

Yevdokimov, Yury M.; Salyanov, V. I.; Кондрашина, О. В.; Borshevsky, Valentin I.; Semenov, S.; Gasanov, A. A.; Reshetov, I.; Kuznetsov, Vjacheslav D.; Никифоров, В.Н.; Akulinichev, S. V.; Mordovskoi, M. V.; Potashev, S. I.; Skorkin, V. M.

doi:10.1016/j.ijbiomac.2005.10.001

Cited by 25 publications

(26 citation statements)

References 29 publications

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“…The results of AFM measurements show that treatment of ds DNA CLCD particles with the gadolinium salt is not accompanied by alteration in the mean size of the "rigid" NaCs of (DNA-Gd) complexes [16][17][18].…”

Section: Resultsmentioning

confidence: 94%

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The Physicochemical and Nanotechnological Approaches to Creation of “Rigid” DNA Nanoconstructions

Yevdokimov¹,

Salyanov²,

Штыкова³

et al. 2014

TONANOJ

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Abstract:Two different approaches to the creation of "rigid" (gel-like) DNA particles (or DNA nanoconstructions) are considered. The physicochemical approach is based on the formation of nanobridges between double-stranded DNA molecules ordered in quasinematic layers of cholesteric liquid-crystalline dispersion (CLCD) particles or their salting-out. This approach represents by itself a "chemical gelation" of DNA molecules realized inside the nanometric DNA particles. The formed "rigid" DNA particles have unique physicochemical properties. The nanotechnological approach is based on induction of "physical gelation" as a result of the formation of Au-clusters in the "free" space between double-stranded DNA molecules ordered in CLCD particles. This approach results in the formation of a new "rigid" DNA material. Its distinctive peculiarity, in contrast to the chemically bonded "rigid" DNA particles, consists in a very weak binding affinity to the nuclear membrane filter used for atomic force microscopy. This opens up a possibility for easy manipulations with CLCD particles doped with gold nanoparticles.Keywords: DNA liquid-crystalline dispersions, nanoconstructions of DNA, nanotechnology of nucleic acids, structure of DNA liquid crystals.

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Section: Resultsmentioning

confidence: 94%

“…These cations were used for realization of this version [16][17][18]. It was shown that the treatment of ds DNA CLCD with gadolinium salt (GdCl 3 ) is accompanied by increase in amplitude of negative abnormal band ( ~ 270 nm) in the CD spectrum [16].…”

Section: Resultsmentioning

confidence: 99%

The Physicochemical and Nanotechnological Approaches to Creation of “Rigid” DNA Nanoconstructions

Yevdokimov¹,

Salyanov²,

Штыкова³

et al. 2014

TONANOJ

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“…As shown in [16], optical properties of CLCD-DNA were demonstrated about it's a liquid crystal structure. Cholesteric liquid crystal dispersion of DNA was formed at the phase deletion of DNA with PEG in the hyperosmotic solution.…”

Section: Visualization Of Clcd-dna-gd Nanoparticlesmentioning

confidence: 95%

“…After the depolymerization, the molecular weight of the DNA was measured in a 1% agarose gel (0,5-0,8) ×10 6 Da using the electrophoretic method. The formation of a cholesteric liquid crystalline dispersion of DNA (CLCD-DNA) in polyethylene glycol has performed the procedure described by Y. M. Yevdokimov et al [15,16]. The formation of CLCD DNA was assessed by the presence of an intense negative band in the circular dichroism spectrum at λ-270 nm.…”

Section: General Proceduresmentioning

confidence: 99%

“…The phenomenon of CLCD-DNA-Gd particles adhesion in cell membranes, presumably, can provide the basis for the development of a technique for the retention gadolinium in tumor tissue, until the neutrons irradiate it. During the interaction of Gd 3+ ions with molecules of double-stranded DNA fixed to the spatial structure of the liquid crystal dispersion with Gd 3+ ions, the cholesteric dispersion structure was retained [16]. Quantitative determination of the concentrations of Gd 3+ in obtained CLCD-DNA-Gd complexes was performed using atomic emission spectral analysis.…”

Section: Immobilization Of Clcd-dna-gd With Macrophages Membranementioning

confidence: 99%

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Dna-Based Hybrid Liquid Crystalline Nano Organometallic Composites for Targeted Drug Delivery in Neutron Capture Therapy

Slivkina

Кондрашина

et al. 2017

Int J Pharm Pharm Sci

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Objective: The aim of this work was focused on the obtaining a DNA-based hybrid nano liquid crystalline organometallic composites for targeted drug delivery in neutron capture therapy. Methods:The formation of a cholesteric liquid crystalline DNA (CLCD-DNA) dispersion has performed using ultrasonic depolymerized calf thymus DNA(MW-0.6-0.8×10 6 Da)2×10 -7 M, mixing with polyethylene glycol 8.5×10 -5 M, dissolved in the 0.3 M NaCl solution and incubated over 1,0 h at 20 °C. CLCD-DNA formation process was determined and controlled by measuring absorption value of circular dichroism (CD) spectrum in the range of 350-600 nm. The organometallic DNA-Gd compound was formed by processing the obtained CLCD-DNA with 2.49 × 10 -4 M, 4.97 × 10 -4 M, 9.8 × 10 -4 M, 1.48× 10 -3 M, 2.92 × 10 -3 M GdCl3 aqueous solutions. CLCD-DNA-gadolinium complex formation process was registered over the appearance of 2 nd amplitude in the CD spectrum. DNA-Gd complex dispersion toxicity was evaluated over 40 min, 24h and 72h incubation with 1×10 6 cells in the RPMI-40 medium supplemented with 3×10 -4 M Gd 3+ . Results:The obtained CLCD-DNA-Gd (d~500 nm) particles was shown less toxicity and higher viability percentage of macrophages after 40 min incubation with CLCD-DNA-Gd contains Gd 3+ ions concentration 3×10 -4 M showed 100% viability and after 72 h ours approximately 89%. The cells immobilized with CLCD-DNA-Gd particles contains 3×10 -4 M Gd 3+ , neutron irritation was caused a 100% cell deaths. Conclusion:Obtained relatively stable, non-cytotoxic drug forms with a maximum local concentration of gadolinium (400 µg/ml). The effectiveness of these nanosystems for targeted drug delivery has markedly superior efficacy than 10 B and other products were based on gadolinium.

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Observations of three “re-entrant” twisted structures in double-stranded DNA dispersion particles

et al. 2021

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Particles of liquid–crystalline dispersions formed by (nucleic acid–rare earth element) complexes as a potential platform for neutron capture therapy

Cited by 25 publications

References 29 publications

The Physicochemical and Nanotechnological Approaches to Creation of “Rigid” DNA Nanoconstructions

The Physicochemical and Nanotechnological Approaches to Creation of “Rigid” DNA Nanoconstructions

Dna-Based Hybrid Liquid Crystalline Nano Organometallic Composites for Targeted Drug Delivery in Neutron Capture Therapy

Observations of three “re-entrant” twisted structures in double-stranded DNA dispersion particles

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