An obvious characteristic of computer games is stress, competition, and physiological alterations in the body. It is regarded as an important subject including those factors in e-sports, getting progressively popular in the digital era. The psychological and physiological impacts of e-sports, conducted in a competitive ambiance, on the body and mind over the course of competitions are not yet known. The aim of the paper is determining the impacts of players performing in e-sports games on some biochemical and hormonal factors shaped in the body during the game. In the group of research, there are twenty electronic sports volunteer players playing in e-sports games. Samples are gathered from the players in the study thirty minutes before and after electronic sports games. Given the results, it is concluded that e-sports players' ACTH, cortisol, and testosterone levels rose substantially.