Partition Chromatography of Yeast and Escherichia coli Soluble Ribonucleic Acid. Relation of Coding Properties to Fractionation^*

Abstract

Escherichia coli and yeast soluble ribonucleic acid (s-RNA) were chromatographed on Sephadex G-25 using the Zachau solvent system. The distribution patterns of amino acid specific s-RNA's from the two sources were similar in general but did show some differences, the most pronounced of which were in the behavior of s-RNA's specific for histidine, tryptophan, proline, and aspartic acid sRNA's. E. coli amino acid specific s-RNA's show a tendency to fractionate according to the amino acid codewords. Yeast s-RNA's demonstrate this tendency also but to a lesser extent.