Partition of perfluoroalkyl substances (PFASs) in whole blood and plasma, assessed in maternal and umbilical cord samples from inhabitants of arctic Russia and Uzbekistan

Hanssen, Linda; Dudarev, Alexey A.; Huber, Sandra; Odland, Jon Øyvind; Nieboer, Evert; Sandanger, Torkjel M.

doi:10.1016/j.scitotenv.2013.01.029

Cited by 139 publications

(121 citation statements)

References 38 publications

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“…Short-chain (PFBA and PFPeA) and long-chain PFASs (PFTrA, PFTeA, PFHxDA and PFODA) were not all detected in several previous studies due to poor peak shape or blank contamination, which resulted in unsatisfactory LODs or recoveries. In the present study, the LODs of short-chain PFASs (PFBA and PFPeA) and long-chain PFASs (PFTrA, PFTeA, PFHxDA and PFODA) were 0.008, 0.022, 0.045, 0.058, 0.15 and 0.19 ng mL −1 , respectively, which were much lower than those of previous studies [1,11,23,25,27,35], the LODs of PFBA, PFPeA, PFTrA, PFTeA, PFHxDA and PFODA were 0.35, 0.76, 0.32, 0.22, 0.53 and 0.42 ng mL −1 , respectively, in Llorca et al's study [27], and the LODs of PFBA, PFPeA, PFTrA, PFTeA, PFHxDA and PFODA in our traditional MTBE method were 0.035, 0.031, 0.19, 0.23, 0.27 and 0.32 ng mL −1 , respectively. The low LODs of short-and long-chain PFASs in the present study might result from our efforts to improve the peak shape of analytes and blank contamination control during the detection process.…”

Section: Validation Of the Methods And Performance With Serum Samplescontrasting

confidence: 80%

“…However, in contrast with the analysis of the standard solutions in acetonitrile or water, the serum samples always showed matrix effects. In present study, 13 isotope-labeled internal standards have been used to correct matrix effects on ionization efficiency as well as to compensate for variation in injection, signal variation due to ion suppression or signal enhancement [4,5,14,16,23,26].…”

Section: Validation Of the Methods And Performance With Serum Samplesmentioning

confidence: 99%

“…High performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) is the most common method for analyzing these compounds [4,5,16,[23][24][25][26], which has good sensitivity and low detection limits for PFASs. Liquid-liquid extraction (LLE) was performed with an ion-pairing agent, such as tetra-nbutylammonium hydrogen sulfate (TBA), and then extracted using methyl tert-butyl ether (MTBE), followed by solid phase extraction (SPE); the extracted liquid is generally used as a pre-treatment to extract and pre-concentrate PFASs from serum samples [4][5][6]16,23] and was regard as an off-line SPE method for the determination of PFASs in human serum.…”

Section: Introductionmentioning

confidence: 99%

“…Liquid-liquid extraction (LLE) was performed with an ion-pairing agent, such as tetra-nbutylammonium hydrogen sulfate (TBA), and then extracted using methyl tert-butyl ether (MTBE), followed by solid phase extraction (SPE); the extracted liquid is generally used as a pre-treatment to extract and pre-concentrate PFASs from serum samples [4][5][6]16,23] and was regard as an off-line SPE method for the determination of PFASs in human serum. However, the off-line SPE method was time-consuming and solvent-intensive and may introduce errors during the manual pre-treatment process.…”

Section: Introductionmentioning

confidence: 99%

“…The polarity and hydrophilicity of the PFASs varied with the carbon chain length and the different functional groups; therefore, simultaneously detecting a wide range of PFASs (C4-C18) was a challenge, especially for shorter (PFBA, perfluoropentanoic acid (PFPeA), and PFBS) and longer chain PFASs (PFHxDA and PFODA). PFBA, PFPeA and PFBS usually showed poor chromatographic peak intensities or unsatisfactory recoveries during the analytical process; PFHxDA and PFODA usually had low spiked recoveries (less than 50%), which made them difficult to determine [1,23,28].…”

Section: Introductionmentioning

confidence: 99%

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A rapid and fully automatic method for the accurate determination of a wide carbon-chain range of per- and polyfluoroalkyl substances (C4–C18) in human serum

Gao

et al. 2016

Journal of Chromatography A

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a b s t r a c tA rapid and fully automatic method for determining 21 per-and polyfluoroalkyl substances (with carbon chains ranging from C4 to C18, including 13 PFCAs, 5 PFSAs, 2 Cl-PFESAs, and PFOSA) in human serum samples was developed. The HPLC parameters, Turboflow column, mobile phase, sample injection volume, loading flow rate, and sample cleanup and elution time were optimized. 25 L serum sample was directly injected into the developed on-line Turboflow SPE HPLC-MS/MS system for analysis after dilution. Matrix effects were corrected due to the matrix removal efficiency of the Turboflow column and sufficient types of internal isotope standards that were used. The established method showed a good linearity (r 2 > 0.99), rapid processing time (20 min per sample), satisfactory recoveries (matrix spiked recoveries range from 84.6% to 114%) and precision (intra-day and inter-day RSDs ranged from 1.5% to 9.2% and from 1.1% to 7.0%, respectively). The limits of detection (LODs) of the 21 analyzed PFASs were between 0.008 and 0.19 ng mL −1 . The LODs of short-and long-chain PFASs, such as PFBA, PFPeA, PFHxDA, and PFODA, were 0.008, 0.022, 0.15 and 0.19 ng mL −1 , respectively; the spiked recoveries of these PFASs were 101, 105, 87.1, and 85.8%, respectively. Both the LODs and recoveries were better than previous studies. Further, serum PFASs concentrations detected by the presented on-line SPE method were consistent with the traditional off-line SPE method (r: 0.98-0.99), which verified the accuracy and applicability of the present method. The method shows good practical prospects in the analysis of trace per-and polyfluoroalkyl substances in human serum.

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Section: Validation Of the Methods And Performance With Serum Samplescontrasting

confidence: 80%

Section: Validation Of the Methods And Performance With Serum Samplesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A rapid and fully automatic method for the accurate determination of a wide carbon-chain range of per- and polyfluoroalkyl substances (C4–C18) in human serum

Gao

et al. 2016

Journal of Chromatography A

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DNA damage in Arctic seabirds: Baseline, sensitivity to a genotoxic stressor, and association with organohalogen contaminants

Haarr

Hylland

Eckbo

et al. 2018

Enviro Toxic and Chemistry

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Environmental contaminants are found throughout Arctic marine ecosystems, and their presence in seabirds has been associated with toxicological responses. However, there are few studies of genotoxicity in Arctic avian wildlife. The purpose of the present study was to quantify deoxyribonucleic acid (DNA) damage in lymphocytes of selected seabird species and to examine whether accumulation of organohalogen contaminants (ΣOHCs) affects DNA damage. Blood was sampled from common eider (Somateria mollissima), black guillemot (Cepphus grylle), black-legged kittiwake (Rissa tridactyla), glaucous gull (Larus hyperboreus), arctic skua (Stercorarius parasiticus), and great skua (Stercorarius skua) in Kongsfjorden, Svalbard (Norway). Contaminant concentrations found in the 6 species differed, presumably because of foraging ecology and biomagnification. Despite large differences in contaminant concentrations, ranging from ΣOHCs 3.3 ng/g wet weight in the common eider to ΣOHCs 895 ng/g wet weight in the great skua, there was no strong difference among the species in baseline DNA damage or sensitivity to a genotoxic stressor (hydrogen peroxide). Baseline levels of DNA damage were low, with median values ranging from 1.7% in the common eider to 8.6% in the great skua. There were no associations between DNA damage and contaminants in the investigated species, suggesting that contaminant concentrations in Kongsfjorden are too low to evoke genotoxic effects, or possibly that lymphocytes are resistant to strand breakage. Clearly, genotoxicity is a topic for future studies of Arctic seabirds. Environ Toxicol Chem 2018;37:1084-1091. © 2017 SETAC.

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Organochlorines, perfluoroalkyl substances, mercury, and egg incubation temperature in an Arctic seabird: Insights from data loggers

Blévin

Shaffer

Bustamante

et al. 2018

Enviro Toxic and Chemistry

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In birds, incubation-related behaviors and brood patch formation are influenced by hormonal regulation such as prolactin secretion. Brood patch provides efficient heat transfer between the incubating parent and the developing embryo in the egg. Importantly, several environmental contaminants are already known to have adverse effects on avian reproduction. However, relatively little is known about the effect of contaminants on incubation temperature (T ) in wild birds. By using temperature thermistors placed into artificial eggs, we investigated whether the most contaminated parent birds are less able to provide appropriate egg warming and thus less committed to incubating their clutch. Specifically, we investigated the relationships among 3 groups of contaminants (organochlorines, perfluoroalkyl substances [PFASs], and mercury [Hg]) with T and also with prolactin concentrations and brood patch size in incubating Arctic black-legged kittiwakes (Rissa tridactyla). Our results reveal that among the organochlorines considered, only blood levels of oxychlordane, the main metabolite of chlordane, a banned pesticide, were negatively related to the minimum incubation temperature in male kittiwakes. Levels of PFASs and Hg were unrelated to T in kittiwakes. Moreover, our study suggests a possible underlying mechanism: since we reported a significant and negative association between blood oxychlordane concentrations and the size of the brood patch in males. Finally, this reduced T in the most oxychlordane-contaminated kittiwakes was associated with a lower egg hatching probability. Environ Toxicol Chem 2018;37:2881-2894. © 2018 SETAC.

show abstract

Partition of perfluoroalkyl substances (PFASs) in whole blood and plasma, assessed in maternal and umbilical cord samples from inhabitants of arctic Russia and Uzbekistan

Cited by 139 publications

References 38 publications

A rapid and fully automatic method for the accurate determination of a wide carbon-chain range of per- and polyfluoroalkyl substances (C4–C18) in human serum

A rapid and fully automatic method for the accurate determination of a wide carbon-chain range of per- and polyfluoroalkyl substances (C4–C18) in human serum

DNA damage in Arctic seabirds: Baseline, sensitivity to a genotoxic stressor, and association with organohalogen contaminants

Organochlorines, perfluoroalkyl substances, mercury, and egg incubation temperature in an Arctic seabird: Insights from data loggers

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