Partitioning of  Geotrichum candidum  Lipase from fermentative crude extract by aqueous two-phase system of polyethylene glycol and sodium citrate

Mazzeu, Cristina Junqueira; Ramos, Elisa Zaparoli; Cavalcanti, Marcello Henrique da Silva; Hirata, Daniela B.; Virtuoso, Luciano Sindra

doi:10.1016/j.seppur.2015.09.069

Cited by 13 publications

(5 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, for practical application, crude enzyme extracts have to be purified to improve their apparent activity and catalytic efficiency [15]. The reported enzyme purification processes such as multi-step solvent partitioning [16], the aqueous two-phase system [17], ammonium sulfate precipitation [18] and sequential chromatographic process [19,20] are tedious and time-consuming. In addition, these steps also incur extra downstream processing costs.…”

Section: Introductionmentioning

confidence: 99%

Direct immobilization of laccase on titania nanoparticles from crude enzyme extracts of P. ostreatus culture for micro-pollutant degradation

Nguyen

Hou

et al. 2017

Separation and Purification Technology

140

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Direct immobilization of laccase on titania nanoparticles from crude enzyme extracts of P. ostreatus culture for micro-pollutant degradation

Nguyen

Hou

et al. 2017

Separation and Purification Technology

140

View full text Add to dashboard Cite

show abstract

“…The results showed that the more the olive waste is diluted the more the solution becomes colorless. The use of additives (salt and polyethylene glycol) had a positive effect on the decrease in the color intensity of the olive oil wastewater due mostly to the decrease in the concentration of total phenols and o-diphenols [44].…”

Section: Resultsmentioning

confidence: 99%

Phenolics decontamination of olive mill wastewater using onion solid by-products homogenate

Reheem¹,

Yılmaz²,

Elhag³

2019

Desalination and Water Treatment

View full text Add to dashboard Cite

a b s t r a c tThe aim of this article was to treat olive oil mill waste with onion peroxidase with regard to the removal of polyphenols and decolonization. Determination of the optimum conditions for the treatment of olive mill wastewater was another objective of this study. Results showed a pH optimum of around 2.65 and a concentration of H 2 O 2 of around 2.58 mM. Moreover, o-diphenols could be removed in a short period of time in comparison to the total phenols. The results showed that three hours were sufficient for 75% removal of o-diphenols. Dilution 1 over 20 of the olive mill wastewaters and the addition of some additives (NaCl and polyethylene glycol) in the treatment of olive mill wastewater (OMW) could enhance the efficiency of total phenol removal as well as, a decrease in the color intensity of the olive oil wastewater. In addition, high performance liquid chromatography (HPLC) analyzes were carried out on samples representing OMW before and after the treatment. Results showed that the degradation of some phenols was carried out with the use of onion peroxidase, as well as with the use of the optimal conditions regarding the pH and the concentration of hydrogen peroxide. It was concluded that onion peroxidase could catalyze the degradation of total phenols and o-diphenols and that such degradation could also influence the change in color and the antioxidant activity of the olive oil mill wastewater.

show abstract

“…Tie line length (TLL) is estimated as square root of the sum of squares of the difference in IL and salt concentrations between the top and bottom phases, inferred from phase diagrams. It is a thermodynamic parameter that expresses the difference between the intensive thermodynamic properties of the two phases of the ATPS [19]. The TLL of a given total phase composition of the system was calculated according to the following equation:…”

Section: Methodsmentioning

confidence: 99%

Extraction of tetracycline using the ionic liquid-based aqueous two phase systems: Single-stage versus multi-stage

Liu

Chen

Zhou³

et al. 2018

CI&CEQ

View full text Add to dashboard Cite

show abstract

Partitioning of Geotrichum candidum Lipase from fermentative crude extract by aqueous two-phase system of polyethylene glycol and sodium citrate

Cited by 13 publications

References 33 publications

Direct immobilization of laccase on titania nanoparticles from crude enzyme extracts of P. ostreatus culture for micro-pollutant degradation

Direct immobilization of laccase on titania nanoparticles from crude enzyme extracts of P. ostreatus culture for micro-pollutant degradation

Phenolics decontamination of olive mill wastewater using onion solid by-products homogenate

Extraction of tetracycline using the ionic liquid-based aqueous two phase systems: Single-stage versus multi-stage

Contact Info

Product

Resources

About