Partitioning recombinant chitinase from Nicotiana benthamiana by an aqueous two-phase system based on polyethylene glycol and phosphate salts

Tue, Nguyen Hoang; Phuc, Nguyen Hoang; Hoa, Phung Thi Bich; Tien, Nguyen Quang Duc; Loc, Nguyen Hoang

doi:10.1016/j.ijbiomac.2024.131924

International Journal of Biological Macromolecules

2024

DOI: 10.1016/j.ijbiomac.2024.131924

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Partitioning recombinant chitinase from Nicotiana benthamiana by an aqueous two-phase system based on polyethylene glycol and phosphate salts

Nguyen Hoang Tue,

Nguyen Hoang Phuc,

Phung Thi Bich Hoa

et al.

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Cited by 2 publications

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Antifungal characterizations of a novel endo-β-1,6-glucanase from Flavobacterium sp. NAU1659

Xie,

Shen,

Geng

et al. 2024

Appl Microbiol Biotechnol

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β-1,6-Glucan plays a crucial role in fungal cell walls by linking the outer layer of mannoproteins and the inner layer of β-1,3-glucan, contributing significantly to the maintenance of cell wall rigidity. Therefore, the hydrolysis of β-1,6-glucan by β-1,6-glucanase directly leads to the disintegration of the fungal cell wall. Here, a novel β-1,6-glucanase FlGlu30 was identified from the endophytic Flavobacterium sp. NAU1659 and heterologously expressed in Escherichia coli BL21 (DE3). The optimal reaction conditions of purified FlGlu30 were 50℃ and pH 6.0, resulting in a specific activity of 173.1 U/mg using pustulan as the substrate. The hydrolyzed products of FlGlu30 to pustulan were mainly gentianose within 1 h of reaction. With the extension of reaction time, gentianose was gradually hydrolyzed to glucose, indicating that FlGlu30 is an endo-β-1,6-glucanase. The germination of Magnaporthe oryzae Guy11 spores could not be inhibited by FlGlu30, but the appressorium formation of spores was completely inhibited under the concentration of 250.0 U/mL FlGlu30. The disruptions of cell wall and accumulation of intracellular reactive oxide species (ROS) were observed in FlGlu30-treated M. oryzae Guy11 cells, suggesting the significant importance of β-1,6-glucan as a potential antifungal target and the potential application of FlGlu30. Key points • β-1,6-Glucan is a key component maintaining the rigid structure of fungal cell wall. • β-1,6-Glucanase is an antifungal protein with significant potential applications. • FlGlu30 is the first reported β-1, 6-glucanase derived from Flavobacterium.

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Antifungal characterizations of a novel endo-β-1,6-glucanase from Flavobacterium sp. NAU1659

Xie,

Shen,

Geng

et al. 2024

Appl Microbiol Biotechnol

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Improving the thermostability of 42 kDa chitinase from Trichoderma asperellum SH16 expressed in Nicotiana benthamiana via site-directed mutagenesis

Tien,

Van Thanh,

et al. 2024

Plant Cell Tiss Organ Cult

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Partitioning recombinant chitinase from Nicotiana benthamiana by an aqueous two-phase system based on polyethylene glycol and phosphate salts

Cited by 2 publications

References 58 publications

Antifungal characterizations of a novel endo-β-1,6-glucanase from Flavobacterium sp. NAU1659

Antifungal characterizations of a novel endo-β-1,6-glucanase from Flavobacterium sp. NAU1659

Improving the thermostability of 42 kDa chitinase from Trichoderma asperellum SH16 expressed in Nicotiana benthamiana via site-directed mutagenesis

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