Parvalbumin and Ubiquitin as Potential Biomarkers of Mercury Contamination of Amazonian Brazilian Fish

Vieira, José Cavalcante Souza; Oliveira, Grasieli de; Braga, Camila Pereira; Fernandes, Marcelo; Moraes, Paula Martin de; Buzalaf, Marília Afonso Rabelo; Oliveira, Lincoln Carlos Silva de; Padilha, Pedro de Magalhães

doi:10.1007/s12011-020-02026-w

Cited by 8 publications

(2 citation statements)

References 40 publications

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“…Therefore, the suggested protective role of CBD in maintaining protein homeostasis may also apply to CBD's effect on mRNA stability. Moreover, it is known that the ribosomal protein 27a can participate in the NF-κB activity [ 81 ] while the inhibition of nucleolin is associated with an anti-inflammatory effect [ 82 ]. The action of CBD in lowering the level of these two proteins may also indicate the anti-inflammatory effect of CBD in keratinocytes exposed to H 2 O 2 .…”

Section: Discussionmentioning

confidence: 99%

Protective effects of cannabidiol on the membrane proteins of skin keratinocytes exposed to hydrogen peroxide via participation in the proteostasis network

2021

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Hydrogen peroxide (H 2 O 2 ) is widely used in clinical practice due to its antiseptic properties and its ability to heal wounds. However, due to its involvement in the formation of ROS, H 2 O 2 causes several side effects, including disorders of the metabolism of skin cells and the development of chronic inflammation mediated by oxidative stress. Therefore, this study evaluated the effects of cannabidiol (CBD), a phytocannabinoid known for its antioxidant and anti-inflammatory properties, on the proteome of keratinocyte membranes exposed to H 2 O 2 . Overall, the hydrogen peroxide caused the levels of several proteins to increase, while the treatment with CBD prevented these changes. Analysis of the protein-protein interaction network showed that the significant changes mainly involved proteins with important roles in the proteasomal activity, protein folding processes (regulatory subunit of the proteasome 26S 6A, beta proteasome subunit type 1, chaperonin 60 kDa), protein biosynthesis (40S ribosomal proteins S16, S2 and ubiquitin-S27a), regulation of the redox balance (carbonyl reductase [NADPH] 1 and NAD(P)H [quinone] 1 dehydrogenase) and cell survival (14-3-3 theta protein). Additionally, CBD reduced the total amount of MDA, 4-HNE and 4-ONE-protein adducts. Therefore, we conclude that CBD partially prevents the changes induced by hydrogen peroxide by reducing oxidative stress and maintaining proteostasis networks. Moreover, our results indicate that combination therapy with CBD may bring a promising approach in the clinical use of hydrogen peroxide by preventing its pro-oxidative and pro-inflammatory effect through potential participation of CBD in membrane mediated molecular signaling.

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Section: Discussionmentioning

confidence: 99%

Protective effects of cannabidiol on the membrane proteins of skin keratinocytes exposed to hydrogen peroxide via participation in the proteostasis network

2021

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“…Moreover, in the hepatic and muscular tissue of filhote ( Brachyplatystoma filamentosum ), a giant catfish in the Amazonian rivers, Hg can also bind to RPS27a (de Queiroz et al, 2019). In the muscular tissues of two species of fish ( Prochilodus lineatus and Mylossoma duriventre ) that feed the Amazonian human population, Hg bind to RPS27a with particularly high affinity, and per molecule of RPS27a contains approximately one Hg atoms (J. C. S. Vieira et al, 2020). Therefore, RPS27a may be a biomarker and carrier of Hg, which may be significant for detecting Hg contamination in aquatic organisms and improving health risk assessments.…”

Section: Rps27a: a Mercury Carrier And Biomarkermentioning

confidence: 99%

Multifaceted functions of RPS27a: An unconventional ribosomal protein

Luo

Zhao

Chen

et al. 2022

Journal Cellular Physiology

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The ribosomal protein S27a (RPS27a) is cleaved from the fusion protein ubiquitin–RPS27a (Ub–RPS27a). Generally, Ub and RPS27a are coexpressed as a fusion protein but function independently after Ub is cleaved from RPS27a by a deubiquitinating enzyme. As an RP, RPS27a assembles into ribosomes, but it also functions independently of ribosomes. RPS27a is involved in the development and poor prognosis of various cancers, such as colorectal cancer, liver cancer, chronic myeloid leukemia, and renal carcinoma, and is associated with poor prognosis. Notably, the murine double minute 2/P53 axis is a major pathway through which RPS27a regulates cancer development. Moreover, RPS27a maintains sperm motility, regulates winged aphid indirect flight muscle degeneration, and facilitates plant growth. Additionally, RPS27a is a metalloprotein and mercury (Hg) biomarker. In the present review, we described the origin, structure, and biological functions of RPS27a.

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Mercury metalloproteomic profile in muscle tissue of Arapaima gigas from the Brazilian Amazon

Bataglioli

Queiroz

Vieira

et al. 2022

Environ Monit Assess

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Parvalbumin and Ubiquitin as Potential Biomarkers of Mercury Contamination of Amazonian Brazilian Fish

Cited by 8 publications

References 40 publications

Protective effects of cannabidiol on the membrane proteins of skin keratinocytes exposed to hydrogen peroxide via participation in the proteostasis network

Protective effects of cannabidiol on the membrane proteins of skin keratinocytes exposed to hydrogen peroxide via participation in the proteostasis network

Multifaceted functions of RPS27a: An unconventional ribosomal protein

Mercury metalloproteomic profile in muscle tissue of Arapaima gigas from the Brazilian Amazon

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