Parvalbumin‐producing striatal interneurons receive excitatory inputs onto proximal dendrites from the motor thalamus in male mice

Nakano, Yasutake; Karube, Fuyuki; Hirai, Yasuharu; Kobayashi, Kenta; Hioki, Hiroyuki; Okamoto, Shinichiro; Kubo, Hiroshi; Fujiyama, Fumino

doi:10.1002/jnr.24214

Cited by 14 publications

(15 citation statements)

References 141 publications

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“…One caveat is that the thalamic ChR2-YFP transduction in this latter study was not limited to the Cm-Pf, but appeared to involve most or all of the thalamus. It is thus significant that a very recent report showed a significant innervation of the proximal dendrites of PV-expressing FSIs arising from motor thalamus (Nakano et al, 2018 ) consistent with recent electrophysiological findings (Sciamanna et al, 2015 ; Assous et al, 2017 ; Arias-García et al, 2018 ; Assous and Tepper, 2018 ).…”

Section: Updates On Previously Identified Striatal Gabaergic Interneusupporting

confidence: 82%

Heterogeneity and Diversity of Striatal GABAergic Interneurons: Update 2018

et al. 2018

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Our original review, “Heterogeneity and Diversity of Striatal GABAergic Interneurons,” to which this is an invited update, was published in December, 2010 in Frontiers is Neuroanatomy. In that article, we reviewed several decades’ worth of anatomical and electrophysiological data on striatal parvalbumin (PV)-, neuropeptide Y (NPY)- and calretinin(CR)-expressing GABAergic interneurons from many laboratories including our own. In addition, we reported on a recently discovered novel tyrosine hydroxylase (TH) expressing GABAergic interneuron class first revealed in transgenic TH EGFP reporter mouse line. In this review, we report on further advances in the understanding of the functional properties of previously reported striatal GABAergic interneurons and their synaptic connections. With the application of new transgenic fluorescent reporter and Cre-driver/reporter lines, plus optogenetic, chemogenetic and viral transduction methods, several additional subtypes of novel striatal GABAergic interneurons have been discovered, as well as the synaptic networks in which they are embedded. These findings make it clear that previous hypotheses in which striatal GABAergic interneurons modulate and/or control the firing of spiny neurons principally by simple feedforward and/or feedback inhibition are at best incomplete. A more accurate picture is one in which there are highly selective and specific afferent inputs, synaptic connections between different interneuron subtypes and spiny neurons and among different GABAergic interneurons that result in the formation of functional networks and ensembles of spiny neurons.

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Section: Updates On Previously Identified Striatal Gabaergic Interneusupporting

confidence: 82%

Heterogeneity and Diversity of Striatal GABAergic Interneurons: Update 2018

et al. 2018

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“…Sections were then washed in TBS for 3 min × 10 min and incubated for 72 h in primary antibody specific for glutamate receptors and transporters at 4 • C ( Table 1). Specificity of the primary antibodies has been tested using western blotting and reported previously for each of the antibodies GluA1 (Zhu et al, 2017;Song et al, 2019), GluA2 (Banerjee et al, 2013;Hussain and Davanger, 2015), GluN1 (Morimura et al, 2017;Seigneur and Südhof, 2018), GluN2A (Atkin et al, 2015;Konstantoudaki et al, 2016), VGluT1 (Venniro et al, 2017;Nakano et al, 2018), VGluT2 (Hernández et al, 2015;Nakano et al, 2018), and Aβ 1−42 (Kwakowsky et al, 2016) (Figures 1A-D). Following 3 min × 10 min washes in TBS, the sections were incubated at RT for 1 h in secondary antibodies goat anti-mouse Alexa Fluor 647 (1:500, A21236, Thermo Fisher, Waltham, MA, United States), goat anti-rabbit Alexa Fluor 488 (1:500, A11034, Thermo Fisher), and goat anti-guinea pig Alexa Fluor 594 (1:500, A11076, Thermo Fisher) diluted in TTB.…”

Section: Fluorescent Immunohistochemistrymentioning

confidence: 97%

The Acute Effects of Amyloid-Beta1–42 on Glutamatergic Receptor and Transporter Expression in the Mouse Hippocampus

et al. 2020

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Alzheimer's disease (AD) is the leading type of dementia worldwide. Despite an increasing burden of disease due to a rapidly aging population, there is still a lack of complete understanding of the precise pathological mechanisms which drive its progression. Glutamate is the main excitatory neurotransmitter in the brain and plays an essential role in the normal function and excitability of neuronal networks. While previous studies have shown alterations in the function of the glutamatergic system in AD, the underlying etiology of beta amyloid (Aβ 1−42) induced changes has not been explored. Here we have investigated the acute effects of stereotaxic hippocampal Aβ 1−42 injection on specific glutamatergic receptors and transporters in the mouse hippocampus, using immunohistochemistry and confocal microscopy 3 days after Aβ 1−42 injection in aged male C57BL/6 mice, before the onset of neuronal cell death. We show that acute injection of Aβ 1−42 is sufficient to induce cognitive deficits 3 days post-injection. We also report no significant changes in glutamate receptor subunits GluA1, GluA2, VGluT1, and VGluT2 in response to acute injection of Aβ 1−42 when compared with the ACSF-vehicle injected mice. However, we observed increased expression in the DG hilus and ventral stratum (str.) granulosum, CA3 str. radiatum and str. oriens, and CA1 str. radiatum of the GluN1 subunit, and increased expression within the CA3 str. radiatum and decreased expression within the DG str. granulosum of the GluN2A subunit in Aβ 1−42 injected mice compared to NC, and a similar trend observed when compared to ACSF-injected mice. We also observed alterations in expression patterns of glutamatergic receptor subunits and transporters within specific layers of hippocampal subregions in response to a microinjection stimulus. These findings indicate that the pathological alterations in the glutamatergic system observed in AD are likely to be partially a result of both acute and chronic exposure to Aβ 1−42 and implies a much more complex circuit mechanism associated with glutamatergic dysfunction than simply glutamate-mediated excitotoxic neuronal death.

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“…41. Model data are in black, experimental data in red.composition, with cell density following continuous spatial gradients specific for each cell type(61)(62)(63)(64)(65). No clear anatomical distinction has been made between the functionally specialized areas of the striatum selective for different cortical and thalamic afferents(66)(67)(68)(69).…”

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confidence: 99%

The microcircuits of striatum in silico

Hjorth

Kozlov

Carannante

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

151

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The basal ganglia play an important role in decision making and selection of action primarily based on input from cortex, thalamus, and the dopamine system. Their main input structure, striatum, is central to this process. It consists of two types of projection neurons, together representing 95% of the neurons, and 5% of interneurons, among which are the cholinergic, fast-spiking, and low threshold-spiking subtypes. The membrane properties, soma–dendritic shape, and intrastriatal and extrastriatal synaptic interactions of these neurons are quite well described in the mouse, and therefore they can be simulated in sufficient detail to capture their intrinsic properties, as well as the connectivity. We focus on simulation at the striatal cellular/microcircuit level, in which the molecular/subcellular and systems levels meet. We present a nearly full-scale model of the mouse striatum using available data on synaptic connectivity, cellular morphology, and electrophysiological properties to create a microcircuit mimicking the real network. A striatal volume is populated with reconstructed neuronal morphologies with appropriate cell densities, and then we connect neurons together based on appositions between neurites as possible synapses and constrain them further with available connectivity data. Moreover, we simulate a subset of the striatum involving 10,000 neurons, with input from cortex, thalamus, and the dopamine system, as a proof of principle. Simulation at this biological scale should serve as an invaluable tool to understand the mode of operation of this complex structure. This platform will be updated with new data and expanded to simulate the entire striatum.

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Parvalbumin‐producing striatal interneurons receive excitatory inputs onto proximal dendrites from the motor thalamus in male mice

Cited by 14 publications

References 141 publications

Heterogeneity and Diversity of Striatal GABAergic Interneurons: Update 2018

Heterogeneity and Diversity of Striatal GABAergic Interneurons: Update 2018

The Acute Effects of Amyloid-Beta1–42 on Glutamatergic Receptor and Transporter Expression in the Mouse Hippocampus

The microcircuits of striatum in silico

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