Parvovirus

Green, Linda K.; Fraire, Armando E.

doi:10.1007/978-3-642-40605-8_16

Cited by 2 publications

(2 citation statements)

References 51 publications

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“…We also found that the type II pneumocytes harbored the CBuV, as it was found colabeling in TTF-1 and panCK-positive cells, and some infected cells were proliferating. Desquamated or reactive type II pneumocytes have been found in pulmonary manifestation of parvovirus B-19 infection in humans and mink infected with Aleutian mink disease parvovirus; 2,13 however, the significance of this finding is unknown. As CBuV has also been found to be colocalized with B-cells and macrophages as it was colabeled with CD20 and Iba-1 IHC, respectively, we speculate that CBuV may harbor these cells in the lung and tracheobronchial lymph node, and the virus may simply replicate in lymphoid tissues, as evident by several studies indicating parvovirus replication.…”

Section: Discussionmentioning

confidence: 99%

“…Four episodes of fatal disease outbreaks occurred in multiple colonies of 2 breeds of dogs, pit bulls (colonies A [cases 1-4 and 6-11] and C [cases [13][14]) and Pomeranians and a pit bull (colony B, cases 5 and 12) on a breeding farm in Thailand from May 2021 to April 2022 (Table 1). In the first episode of a fatal outbreak in pit bull colony A, 6, 35-day-old unvaccinated puppies, and 1 out of 2 completely vaccinated adult dogs developed respiratory distress.…”

Section: Outbreak Informationmentioning

confidence: 99%

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Canine bufavirus (Carnivore protoparvovirus-3) infection in dogs with respiratory disease

Piewbang,

Poonsin,

Lohavicharn

et al. 2023

Vet Pathol

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Canine bufavirus (CBuV) or Carnivore protoparvovirus-3, a nonenveloped DNA virus belonging to the genus Protoparvovirus, family Parvoviridae, has been identified in dogs with respiratory and enteric diseases. Although CBuV detection has been reported in multiple countries, descriptions of pathologic findings associated with infection have not yet been provided. In this study, the authors necropsied 14 dogs (12 puppies and 2 adult dogs) from a breeding colony that died during multiple outbreaks of respiratory diseases. Postmortem investigations revealed extensive bronchointerstitial pneumonia with segmental type II pneumocyte hyperplasia in all necropsied puppies but less severe lesions in adults. With negative results of common pathogen detection by ancillary testing, CBuV DNA was identified in all investigated dogs using a polymerase chain reaction (PCR). Quantitative PCR demonstrated CBuV DNA in several tissues, and in situ hybridization (ISH) indicated CBuV tissue localization in the lung, tracheobronchial lymph node, and spinal cord, suggesting hematogenous spread. Dual CBuV ISH and cellular-specific immunohistochemistry were used to determine the cellular tropism of the virus in the lung and tracheobronchial lymph node, demonstrating viral localization in various cell types, including B-cells, macrophages, and type II pneumocytes, but not T-cells. Three complete CBuV sequences were successfully characterized and revealed that they clustered with the CBuV sequences obtained from dogs with respiratory disease in Hungary. No additional cases were identified in small numbers of healthy dogs. Although association of the bufavirus with enteric disease remains to be determined, a contributory role of CBuV in canine respiratory disease is possible.

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Section: Discussionmentioning

confidence: 99%

Section: Outbreak Informationmentioning

confidence: 99%