The addition of substrates to small instream obstacles, like low-head ramped weirs, has been considered a useful management solution to retrofit those structures and enhance fish passage. Substrate dimensions and spatial arrangement, together with discharge, and consequently water depths, appear as important factors for the creation of hydrodynamic conditions that may facilitate the successful passage of fish, though related studies are scarce to support decision-making. This study assessed the influence of discharge (Q) and different retrofitting designs (RD) on the upstream passage performance of a potamodromous cyprinid, the Iberian barbel (Luciobarbus bocagei). Different substrates (small boulders, large boulders, cobbles) and spatial arrangements (aligned, offset) were tested. Numerical modelling was performed to characterize the hydrodynamics. Results indicate that Q and RD influenced the upstream negotiation of ramped weirs. Cobbles randomly distributed along the ramp (Nature design) was the most successful configuration, recording the highest number of upstream passages. Low velocities along the ramp, and low turbulence downstream, were registered in this configuration, indicating that the use of natural substrate may help to increase the permeability of ramped weirs to fish movements. The outcomes from this work can help engineers and biologists to design more appropriate passage structures for low-head instream obstacles.2 of 18 after assessment, could not be removed, have undergone rehabilitation works to include ramps in their designs (e.g., [11,12]). This design reconfiguration enables water to pass over the ramp, not generating a waterfall [5,10], a condition that may afford a more holistic negotiation by fish [5,13]. Nevertheless, fish swimming abilities, which are closely related to fish guilds and body size [14][15][16][17], and hydrodynamic conditions, such as water depth, discharge, and turbulence present over the ramp and in the vicinity of the structure [5,[18][19][20], are key factors that influence the permeability of such structures to fish movements.The addition of substrates to instream obstacles and fish transposition devices, commonly referred to as retrofitting, has been considered a useful management solution to enhance upstream fish passage [5,[21][22][23][24][25][26][27][28]. The placement of substrates in culverts to mimic natural stream conditions and facilitate fish passage has been successfully implemented since the early 1970s [29]. In low-head ramped weirs, natural substrates like pebbles, cobbles, or boulders, are frequently used as retrofitting solutions to increase the bed roughness, and consequently promote energy dissipation, creating localized zones of low-velocity and turbulence [23][24][25]30] that may be used by fish as resting areas during the upstream negotiation of ramps [20,27,[31][32][33]. These low velocities may be especially important to species of weaker swimming capabilities, such as potamodromous cyprinids [5,21,22]. Substrate dimensions and spatial arrang...