Passatempo Virus, a Vaccinia Virus Strain, Brazil

Leite, Juliana Almeida; Drumond, Betânia Paiva; Trindade, Giliane S.; Lobato, Zélia Inês Portela; Fonseca, Flávio Guimarães da; Santos, João Rodrigues dos; Madureira, Marieta C.; Guedes, Maria Isabel Maldonado Coelho; Ferreira, Jaqueline Maria Siqueira; Bonjardim, Cláudio Antônio; Ferreira, Paulo César Peregrino; Kroon, Erna Geessien

doi:10.3201/eid1112.050773

Cited by 110 publications

(146 citation statements)

References 13 publications

Supporting

Mentioning

135

Contrasting

Unclassified

Order By: Relevance

“…One virus was isolated and denominated Muriaé virus (MURV). 24 During molecular characterization, when amplifying the ha gene, a deletion of 18 nucleotides was observed, allowing this virus introduction in the group of PSTV, 23 ARAV, 11 GP2V, 10 and CTGV. 27 Despite these similarities, Muriaé virus had unique characteristics that allowed its differentiation from other samples of VACV.…”

Section: Muriaé Virusmentioning

confidence: 99%

“…These factors are believed to contribute to virus dissemination from cattle to milkers and vice-versa. 23 This zoonosis seems to occur seasonally, mainly in the dry season, from July to September. Dry weather conditions seem to favor disease appearance and dissemination, since it contributes to dry cows' teats and milkers' hands, enabling the occurrence of lesions that cause the virus transmission from animals to men and vice-versa.…”

Section: Vaccinia Virus Outbreaks In Brazilmentioning

confidence: 99%

“…23 A fact of great relevance for public health is that physicians and other health care professionals have diffi culty in diagnosing and managing these infections. 24 The clinical manifestations, transmission, and viral diagnosis are shown in Table 1.…”

Section: Clinical Signs Transmission and Viral Diagnosismentioning

confidence: 99%

“…When analyzing the blood of patients, antibodies against VACV-WR were identifi ed. 23 That Vaccinia virus variant, denominated Passatempo virus, has a deletion of 18 nucleotides in ha gene, which represents a genetic signature of some samples found in Brazil. 23 …”

Section: Passatempo Virusmentioning

confidence: 99%

See 3 more Smart Citations

Clinical signs, diagnosis, and case reports of Vaccinia virus infections

Silva¹,

Moreira-Silva²,

Gomes³

et al. 2010

Braz J Infect Dis

View full text Add to dashboard Cite

We declare no confl ict of interest. ABSTRACTVaccinia virus is responsible for a zoonosis that usually affects cattle and human beings in Brazil. The initial clinical signs of the infection are focal red skin areas, fever, and general symptoms similar to those of a cold. Then, pustules and ulcerated lesions surrounded by edema and erythema follow, as well as local lymphadenopathy that can last for weeks. Cure and healing of the lesions occur over several weeks, leaving a typical scar in the skin of people and animals affected. The infection defi nitive diagnosis is made through morphological characterization of the virus by use of electron microscopy, followed by PCR for specifi c viral genes. Since 1963, circulating orthopoxviruses in infectious outbreaks in several regions of Brazil have been reported. Later, the etiological agent of those infections was characterized as samples of Vaccinia virus. In addition, the widespread use of those viruses in research laboratories and mass vaccination of militaries have contributed to increase the cases of those infections worldwide. Thus, several epidemiological and clinical studies are required, as well as studies of viral immunology, public health, and economic impact, because little is known about those Vaccinia virus outbreaks in Brazil.

show abstract

Section: Muriaé Virusmentioning

confidence: 99%

Section: Vaccinia Virus Outbreaks In Brazilmentioning

confidence: 99%

Section: Clinical Signs Transmission and Viral Diagnosismentioning

confidence: 99%

Section: Passatempo Virusmentioning

confidence: 99%

See 2 more Smart Citations

Clinical signs, diagnosis, and case reports of Vaccinia virus infections

Silva¹,

Moreira-Silva²,

Gomes³

et al. 2010

Braz J Infect Dis

View full text Add to dashboard Cite

show abstract

“…Decrease in milk production due to VACV infection may reach 50%. Losses in dairy cattle also include expenses with veterinary care and secondary infections of teats (mastitis) that can also affect milk production (LEITE et al, 2005;LOBATO et al, 2005;LUDOLFO DE OLIVEIRA et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Experimental infection of horses with Vaccinia virus

et al. 2015

View full text Add to dashboard Cite

show abstract

Rapid detection of Orthopoxvirus by semi‐nested PCR directly from clinical specimens: A useful alternative for routine laboratories

Abrahão

Drumond

Trindade

et al. 2010

Journal of Medical Virology

View full text Add to dashboard Cite

Orthopoxvirus (OPV) has been associated with worldwide exanthematic outbreaks, which have resulted in serious economic losses as well as impact on public health. Although the current classical and molecular methods are useful for the diagnosis of OPV, they are largely inaccessible to unsophisticated clinical laboratories. The major reason for the inaccessibility is that they require both virus isolation and DNA manipulation. In this report, a rapid, sensitive and low-cost semi-nested PCR method is described for the detection of OPV DNA directly from clinical specimens. A set of primers was designed to amplify the conserved OPV vgf gene. The most useful thermal and chemical conditions were selected and minimum non-inhibitory dilutions were determined. More than 100 Brazilian Vaccinia virus (VACV) field clinical specimens were tested using this semi-nested PCR in order to confirm its applicability. Cowpox virus was also detected by PCR from the ear scabs of scarified Balb/c mice. In addition, the method was highly sensitive for the detection of VACV DNA in murine blood and excreta, which are among the suggested reservoirs of OPV. Together, these data suggest that semi-nested PCR can be used for initial screening for OPV and as a routine diagnostic laboratory method.

show abstract

Passatempo Virus, a Vaccinia Virus Strain, Brazil

Cited by 110 publications

References 13 publications

Clinical signs, diagnosis, and case reports of Vaccinia virus infections

Clinical signs, diagnosis, and case reports of Vaccinia virus infections

Experimental infection of horses with Vaccinia virus

Rapid detection of Orthopoxvirus by semi‐nested PCR directly from clinical specimens: A useful alternative for routine laboratories

Contact Info

Product

Resources

About