Passivating quantum dots against histag-displaying enzymes using blocking peptides: salient considerations for self-assembling quantum dot biosensors

Green, Christopher M.; Hastman, David A.; Susumu, Kimihiro; Spangler, Joseph; Stenger, David A.; Medintz, Igor L.; Díaz, Sebastián A.

doi:10.1039/d3sd00149k

Sens. Diagn.

2023

DOI: 10.1039/d3sd00149k

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Passivating quantum dots against histag-displaying enzymes using blocking peptides: salient considerations for self-assembling quantum dot biosensors

Christopher M. Green,

David A. Hastman,

Kimihiro Susumu

et al.

Abstract: Peptide-based passivation strategies were developed to prevent the unintended conjugation of his-tagged enzymes to quantum dot (QD) biosensors, enabling the application of QD biosensors in complex assays such as CRISPR-based diagnostics.

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Passivating quantum dots against histag-displaying enzymes using blocking peptides: salient considerations for self-assembling quantum dot biosensors

Abstract: Peptide-based passivation strategies were developed to prevent the unintended conjugation of his-tagged enzymes to quantum dot (QD) biosensors, enabling the application of QD biosensors in complex assays such as CRISPR-based diagnostics.

Cited by 5 publications

References 62 publications

Nanomaterials as signal amplifiers in CRISPR/Cas biosensors: A path toward multiplex point-of-care diagnostics

Nanomaterials as signal amplifiers in CRISPR/Cas biosensors: A path toward multiplex point-of-care diagnostics

High-fidelity CRISPR/Cas13a trans cleavage-driven assembly of single quantum dot nanosensor for ultrasensitive detection of long noncoding RNAs in clinical breast tissues

Non-canonical CRISPR/Cas12a-based technology: A novel horizon for biosensing in nucleic acid detection

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