Passive electrical properties of cultured murine lymphoblast (L5178Y) with reference to its cytoplasmic membrane, nuclear envelope, and intracellular phases

Abstract

Dielectric dispersion measurements over a frequency range 0.01-100 MHz were made with the suspensions of a cultured cell line, mouse lymphoma L5178Y, and an attempt to explain the observed dielectric behavior by taking explicitly into consideration the possible involvement of cell nucleus has been presented. The use of a conventional "single-shell" model in which the cell is represented by a homogeneous sphere coated with a thin limiting shell phase did not duplicate the observed dispersion curves, whereas a "double-shell" model in which one additional concentric shell is incorporated into the "single-shell" model gave a much better fit between the observed and the predicted dispersion curves. Based on the latter model, we analyzed the raw data of dielectric measurements to yield a set of plausible electrical parameters for the lymphoma cell: CM approximately or equal to 1.0 muF/cm2, CN approximately or equal to 0.4 muF/cm2, epsilonk approximately or equal to 300, kc/ka approximately to or equal to 0.9, and kk/kc approximately or equal to 0.7. Here, CM and CN are the specific capacities of plasma and nuclear membranes; epsilon and k are the dielectric constant and conductivity with subscript a, c and k referring respectively to the extracellular, the cytoplasmic and the karyoplasmic phases.

Ultrashort electric pulse induced changes in cellular dielectric properties

“…TDDS enables us to nonintrusively determine the electrical properties of the cell membrane, cytoplasm, nucleus, and nucleoplasm according to the twoshell model of the eukaryotic cell, shown in Fig. 1 [10][11][12]. We note that this model has also been referred to as a ''three-shell model'' [13]; however, we follow the two-shell nomenclature Feldman et al TDDS can rapidly obtain dielectric spectra over a wide frequency range with uncertainties on the order 3-5% and 5-7% for the real and imaginary components of the permittivity [14], which are similar to frequency domain systems [15].…”

Dielectric properties of dipicrylamine-doped erythrocytes, cultured cells and lipid vesicles

“…In this analysis, only the values of  L ,  L / H and C m are required, but it is unnecessary to determine the volume fraction of cells. The values of C m are available from the literatures, i.e., 10 mF/m 2 for L5178Y cells [28] and 7 mF/m 2 for erythrocytes [29][30][31]. Figure 9 shows the values of k i and N t estimated for horse erythrocytes of spherical shape and L5178Y cells as a function of the DPA concentration, together with those so far obtained by ROT with human erythrocytes and cultured cells.…”

Model evaluation of changes in electrorotation spectra of biological cells after nsPEF electroporation

“…In biology, examples of spherical shapes are common, such as vesicles, protoplasts, murine myeloma cells [12] and some bacteria such as Streptococcus [34]. Given the simpler geometry and its prevalent use, a double-shelled spherical model, as used by Irimajiri [35] to model lymphoma cells, will be adopted in this study. Here, we combine a numerical, spatially non-uniform evaluation of the pore formation based on Smoluchowski theory, with a double-shelled model for spherical cells, to predict the overall frequency-dependent Clausius-Mossotti (C-M) factors and thus, the ROT spectrum.…”