Passive Immunization with Anti-Laminin Immunoglobulin G Modifies the Integrity of the Seminiferous Epithelium and Induces Arrest of Spermatogenesis in the Guinea Pig1

Lustig, Livia; Denduchis, Berta; Ponzio, Roberto; Lauzon, Michel; Pelletier, R.‐Marc

doi:10.1095/biolreprod62.6.1505

Cited by 26 publications

(13 citation statements)

References 46 publications

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“…The role of laminin in the male reproduction has been inferred from a number of observations. For instance, passive immunization of guinea pig with antilaminin IgG induced changes in the laminin component of the seminiferous biomatrix that accompany and perhaps cause modifications in Sertoli cells, which no longer can provide an environment adequate for the normal progression of spermatogenesis (Lustig et al 2000). Moreover, thickened basement membrane underlying the seminiferous epithelium is incompatible with normal spermatogenesis and male fertility (Dym 1994).…”

Section: Discussionmentioning

confidence: 99%

Comparative expression of laminin and smooth muscle actin in the testis and epididymis of poultry and rabbit

Abd‐Elmaksoud

2009

J Mol Hist

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The present investigation was conducted to demonstrate laminin and alpha smooth muscle actin (alphaSMA) in the testis and epididymis of adult chickens, Sudani ducks, pigeons, and rabbits. This study may represent the first indication for the presence of laminin in the male reproductive organs of birds and rabbits and might therefore serve as a milestone for further reports. In the testis of chicken, Sudani duck, pigeon, and rabbit, the laminin was localized in the basal lamina of the seminiferous tubules and of the peritubular myoid cells, in the testicular capsule and to a small extent in the vicinity of Leydig cells. The testicular vasculature also exhibited intense laminin immunostaining. Weak laminin staining was additionally seen in the cytoplasm of the duck Sertoli cells. In the epididymis, the basal lamina of the epididymal epithelium showed a distinctly positive reaction in all birds and rabbit. The basal lamina of the periductal myoid cells also showed a positive reaction. In the interductal tissue, laminin immunostaining was particularly observed in chicken, duck and pigeon. Laminin positive reaction was also seen in the epididymal vasculatures of all birds and rabbit. Interestingly, weak to moderate laminin staining was observed in the apical surface of the ciliated cells of the proximal and distal efferent ductules in chicken, duck and pigeon. alphaSMA positive reaction was seen in the testicular capsule and in the peritubular myoid cells of all birds and rabbit. In the testicular capsule, alphaSMA staining was either observed in the inner portion (chicken) or throughout the tunica albuginea (Sudani duck and pigeon), or in the outer aspect (rabbit). Distinct alphaSMA reaction was additionally observed in the testicular vasculature. In the epididymis of all birds and rabbit, the alphaSMA was particularly seen in the periductal and interductal myoid cells as well as in the epididymal vasculatures. No alphaSMA specific staining was however detected in the epididymal epithelium, fibrous lamina propria, and luminal spermatozoa of all birds and rabbits. In conclusion, the distribution of laminin and alphaSMA in the testis and epididymis might point out to their roles in the male reproduction.

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Section: Discussionmentioning

confidence: 99%

Comparative expression of laminin and smooth muscle actin in the testis and epididymis of poultry and rabbit

Abd‐Elmaksoud

2009

J Mol Hist

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“…Seminiferous tubules coordinate with Leydig cells and the brain via the hypothalamic-pituitary-testicular axis to regulate spermatogenesis (Cheng and Mruk, 2002;Mruk and Cheng, 2004). Dynamic cross talk also occurs between cells of the seminiferous epithelium and the extracellular matrix components (Lustig et al, 2000;Siu and Cheng, 2004). These issues are complex and beyond the scope of this review.…”

Section: Testicular Histology: An Overviewmentioning

confidence: 95%

“…Sertoli cells are a major contributor to the SSC niche, but so are myoid and Leydig cells (Oatley and Brinster, 2008) and constituents of the limiting membrane (Lustig et al, 2000). The topographical distribution of spermatogonia has been examined and the A s , A pr , and A al spermatogonial cell populations are located primarily in areas of the seminiferous tubules opposing the interstitial space from stages VI-XII and I-III, as are the A 1 spermatogonia at stage VII and the A 2 spermatogonia at stage IX of the cycle.…”

Section: Importance Of Microenvironment (Niche) Formentioning

confidence: 99%

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 1: Background to spermatogenesis, spermatogonia, and spermatocytes

Hermo

Pelletier

Cyr

et al. 2009

Microscopy Res & Technique

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399

232

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As germ cells divide and differentiate from spermatogonia to spermatozoa, they share a number of structural and functional features that are common to all generations of germ cells and these features are discussed herein. Germ cells are linked to one another by large intercellular bridges which serve to move molecules and even large organelles from the cytoplasm of one cell to another. Mitochondria take on different shapes and features and topographical arrangements to accommodate their specific needs during spermatogenesis. The nuclear envelope and pore complex also undergo extensive modifications concomitant with the development of germ cell generations. Apoptosis is an event that is normally triggered by germ cells and involves many proteins. It occurs to limit the germ cell pool and acts as a quality control mechanism. The ubiquitin pathway comprises enzymes that ubiquitinate as well as deubiquitinate target proteins and this pathway is present and functional in germ cells. Germ cells express many proteins involved in water balance and pH control as well as voltage-gated ion channel movement. In the nucleus, proteins undergo epigenetic modifications which include methylation, acetylation, and phosphorylation, with each of these modifications signaling changes in chromatin structure. Germ cells contain specialized transcription complexes that coordinate the differentiation program of spermatogenesis, and there are many male germ cell-specific differences in the components of this machinery. All of the above features of germ cells will be discussed along with the specific proteins/genes and abnormalities to fertility related to each topic.

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“…Laminin, a component of ECM, is known to regulate Sertoli cell TJ-barrier function possibly by providing the functional linkage between ECM and intracellular cytoskeleton [22]. Also, passive immunization of guinea pigs with antilaminin IgG can perturb spermatogenesis, inducing germ cell loss from the epithelium [23]. …”

Section: Maintain the Seminiferous Epithelium Integritymentioning

confidence: 99%

Extracellular Matrix: Recent Advances on Its Role in Junction Dynamics in the Seminiferous Epithelium During Spermatogenesis1

Siu

Cheng

2004

Biology of Reproduction

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Spermatogenesis takes place in the seminiferous epithelium of the mammalian testis in which one type A1 spermatogonium (diploid, 2n) gives rise to 256 spermatids (haploid, 1n). To accomplish this, developing germ cells, such as preleptotene and leptotene spermatocytes, residing in the basal compartment of the seminiferous epithelium must traverse the blood-testis barrier (BTB) entering into the adluminal compartment for further development into round, elongating, and elongate spermatids. Recent studies have shown that the basement membrane in the testis (a modified form of extracellular matrix, ECM) is important to the event of germ cell movement across the BTB because proteins in the ECM were shown to regulate BTB dynamics via the interactions between collagens, proteases, and protease inhibitors, possibly under the regulation of cytokines. While these findings are intriguing, they are not entirely unexpected. For one, the basement membrane in the testis is intimately associated with the BTB, which represents the basolateral region of Sertoli cells. Also, Sertoli cell tight junctions (TJs) that constitute the BTB are present side-by-side with cell-cell actin-based adherens junctions (AJ, such as basal ectoplasmic specialization [ES]) and intermediate filament-based desmosome-like junctions. As such, the relative morphological layout between TJs, AJs, and desmosome-like junctions in the seminiferous epithelium is in sharp contrast to other epithelia where TJs are located at the apical portion of an epithelium or endothelium, furthest away from ECM, to be followed by AJs and desmosomes, which in turn constitute the junctional complex. For another, anchoring junctions between a cell epithelium and ECM found in multiple tissues, also known as focal contacts (or focal adhesion complex, FAC, an actin-based cell-matrix anchoring junction type), are the most efficient junction type that permits rapid junction restructuring to accommodate cell movement. It is therefore physiologically plausible, and perhaps essential, that the testis is using some components of the focal contacts to regulate rapid restructuring of AJs between Sertoli and germ INTRODUCTIONDuring spermatogenesis, preleptotene and leptotene spermatocytes residing at the basal compartment of the seminiferous epithelium adjacent to the basement membrane must traverse the blood-testis barrier (BTB) at late stage VIII through early stage IX of the epithelial cycle, entering the adluminal compartment for further development ( Figs. 1 and 2) [1][2][3]. These primary spermatocytes continue to differentiate into round, elongating, and eventually elongate spermatids while moving to the luminal edge of the epithelium and to be released into the tubule lumen at spermiation [2]. These events of cell movement across the seminiferous epithelium are also associated with extensive remodeling of actin-based cell-cell and cell-matrix anchoring junctions, also known as adherens junctions (AJs) and focal contacts, respectively; as well as intermediate filament-based ...

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Passive Immunization with Anti-Laminin Immunoglobulin G Modifies the Integrity of the Seminiferous Epithelium and Induces Arrest of Spermatogenesis in the Guinea Pig1

Cited by 26 publications

References 46 publications

Comparative expression of laminin and smooth muscle actin in the testis and epididymis of poultry and rabbit

Comparative expression of laminin and smooth muscle actin in the testis and epididymis of poultry and rabbit

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 1: Background to spermatogenesis, spermatogonia, and spermatocytes

Extracellular Matrix: Recent Advances on Its Role in Junction Dynamics in the Seminiferous Epithelium During Spermatogenesis1

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