Passive laboratory surveillance in Spain: pathogens as risk factors for honey bee colony collapse

Cepero, Almudena; Martín-Hernández, Raquel; Bartolomé, Carolina; Gómez‐Moracho, Tamara; Barrios, Laura; Bernal, José; Martín, Margarita; Meana, Aránzazu; Higes, Mariano

doi:10.1080/00218839.2016.1162978

Cited by 15 publications

(11 citation statements)

References 48 publications

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“…flexonema , and two with respect to those of C. bombi . Considering the low levels of divergence displayed by the SSU ‐locus across trypanosomatid species (Cepero et al ., 2015), it was impossible to ascertain whether this sequence represented a polymorphic variant of any of the former species or corresponded to a new taxon (Trypanosomatidae sp. ; Tsp in Table 1; GenBank accession numbers MW001676 and MW002685 in the Supporting Information Fig.…”

Section: Resultsmentioning

confidence: 99%

Wide diversity of parasites in Bombus terrestris (Linnaeus, 1758) revealed by a high‐throughput sequencing approach

Bartolomé

Jabal-Uriel

Buendía-Abad

et al. 2020

Environmental Microbiology

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Summary Assessing the extent of parasite diversity requires the application of appropriate molecular tools, especially given the growing evidence of multiple parasite co‐occurrence. Here, we compared the performance of a next‐generation sequencing technology (Ion PGM ™ System) in 12 Bombus terrestris specimens that were PCR‐identified as positive for trypanosomatids (Leishmaniinae) in a previous study. These bumblebees were also screened for the occurrence of Nosematidae and Neogregarinorida parasites using both classical protocols (either specific PCR amplification or amplification with broad‐range primers plus Sanger sequencing) and Ion PGM sequencing. The latter revealed higher parasite diversity within individuals, especially among Leishmaniinae (which were present as a combination of Lotmaria passim, Crithidia mellificae and Crithidia bombi), and the occurrence of taxa never reported in these hosts: Crithidia acanthocephali and a novel neogregarinorida species. Furthermore, the complementary results produced by the different sets of primers highlighted the convenience of using multiple markers to minimize the chance of some target organisms going unnoticed. Altogether, the deep sequencing methodology offered a more comprehensive way to investigate parasite diversity than the usual identification methods and provided new insights whose importance for bumblebee health should be further analysed.

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Section: Resultsmentioning

confidence: 99%

Wide diversity of parasites in Bombus terrestris (Linnaeus, 1758) revealed by a high‐throughput sequencing approach

Bartolomé

Jabal-Uriel

Buendía-Abad

et al. 2020

Environmental Microbiology

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“…Finally, the type of foraging flora was confirmed by analyzing beebread samples as previously described [10,19,31] and estimating the proportion of pollen from wild (WP) and cultivated (CP) plants.…”

Section: Stored Pollen Analysismentioning

confidence: 99%

“…[7][8][9]. However, the impact of pathogens on this decline (mainly parasites and related virus) may be particularly important [10][11][12], probably in conjunction with the accumulation of pesticide residues in hive matrices. Thus, the combined effect of two or more such stressors could drive mortality of individuals, eventually leading to the colony collapse [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

A Case Report of Chronic Stress in Honey Bee Colonies Induced by Pathogens and Acaricide Residues

Alonso-Prados¹,

González-Porto²,

Bernal³

et al. 2021

Preprint

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In this Case Report we analyze the possible causes of the poor health status of a professional Apis mellifera iberiensis apiary located in Gajanejos (Guadalajara, Spain). Several factors that potentially favor colony collapse were identified, including Nosema ceranae infection, alone or in combination with other factors (eg, BQCV and DWV infection), and the accumulation of acaricides commonly used to control Varroa destructor in the beebread (coumaphos and tau-fluvalinate). Based on the levels of residues, the average toxic unit estimated for the apiary, suggests a possible increase in vulnerability to infection by N. ceranae due to the presence of high levels of acaricides. These data highlight the importance of evaluating these factors in future monitoring programs, as well as the need to adopt adequate preventive measures as part of national and international welfare programs aimed at guaranteeing the health and fitness of bees.

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“…These changes may be also influenced by the nutrition quality of the collected pollen, which depends on the seasonal climatological conditions [ 8 , 9 , 10 ]. Overall, the impact of pathogens on this decline (mainly parasites and related viruses) may be particularly important [ 11 , 12 , 13 ], probably in conjunction with the accumulation of pesticide residues in hive matrices. Thus, the combined effect of two or more such stressors could drive the mortality of individuals, eventually leading to colony collapse [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

A Case Report of Chronic Stress in Honey Bee Colonies Induced by Pathogens and Acaricide Residues

et al. 2021

Self Cite

View full text Add to dashboard Cite

In this case report, we analyze the possible causes of the poor health status of a professional Apis mellifera iberiensis apiary located in Gajanejos (Guadalajara, Spain). Several factors that potentially favor colony collapse were identified, including Nosema ceranae infection, alone or in combination with other factors (e.g., BQCV and DWV infection), and the accumulation of acaricides commonly used to control Varroa destructor in the beebread (coumaphos and tau-fluvalinate). Based on the levels of residues, the average toxic unit estimated for the apiary suggests a possible increase in vulnerability to infection by N. ceranae due to the presence of high levels of acaricides and the unusual climatic conditions of the year of the collapse event. These data highlight the importance of evaluating these factors in future monitoring programs, as well as the need to adopt adequate preventive measures as part of national and international welfare programs aimed at guaranteeing the health and fitness of bees.

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Passive laboratory surveillance in Spain: pathogens as risk factors for honey bee colony collapse

Cited by 15 publications

References 48 publications

Wide diversity of parasites in Bombus terrestris (Linnaeus, 1758) revealed by a high‐throughput sequencing approach

Wide diversity of parasites in Bombus terrestris (Linnaeus, 1758) revealed by a high‐throughput sequencing approach

A Case Report of Chronic Stress in Honey Bee Colonies Induced by Pathogens and Acaricide Residues

A Case Report of Chronic Stress in Honey Bee Colonies Induced by Pathogens and Acaricide Residues

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