Passive protective ability of the outer membrane protein PF1380 of Pseudomonas fluorescens against the major pathogenic bacteria of freshwater aquaculture in fish

Liu, Xiang; Sun, Wei; Jian, Sijie; Cao, Jia; Chen, Chen; Chen, Chunlin; Chen, Rui; Ding, Rui

doi:10.1016/j.aqrep.2021.100985

Cited by 5 publications

(6 citation statements)

References 32 publications

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“…The offspring produced from the broodstock that were vaccinated with PAPS had the highest antibodies ( Table 2 ) and lysozyme activity ( Figure 4 ) up to the 30 th day post-hatching period and was the lowest in the offsprings from the unvaccinated broodstock (P<0.05). This demonstrated that their strong immune response was transferred to their offsprings 27 – 29 , 33 , 34 , 52 through the egg yolk 53 .…”

Section: Discussionmentioning

confidence: 98%

“…The types of bacterial diseases studied in the aforementioned studies are very limited even though Nile tilapia often suffer from them in fish farms and hatcheries 35 . Besides being infected by S. agalactiae 29 , 34 – 36 , Nile tilapia are often infected by A. hydrophila 9 , 35 , 37 and P. fluorescens 37 , 38 leading to high mortality, including in Indonesia. Therefore, this study aimed to examine maternal immunity transfer using the polyvalent vaccine for S. agalactiae , A. hydrophila, and P. fluorescens (PAPS).…”

Section: Introductionmentioning

confidence: 99%

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Transfer of maternal immunity using a polyvalent vaccine and offspring protection in Nile tilapia, Oreochromis niloticus

Amrullah,

Wahidah,

Ardiansyah

et al. 2023

F1000Res

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Background Vaccination is an effective and alternative means of disease prevention, however, it cannot be conducted on the offspring of fish. For this process to take place, the transfer of maternal immunity should be implemented. This study aims to determine the effectiveness of transferring immunity from the broodstock to the offspring using a polyvalent vaccine against Aeromonas hydrophila, Streptococcus agalactiae, and Pseudomonas fluorescens in Nile tilapia, Oreochromis niloticus. Methods Nile tilapia broodstock with an average weight of 203g (±SD 23) was reared in spawning ponds until mass spawning and harvested one week post-spawning for vaccination. After being vaccinated according to the treatment, each fish broodstock was reared in 3x3 m cages installed in an earthen pond with a density of 20 broodstock, consisting of 15 females and 5 males. The vaccine used was a formalin-killed whole-cell vaccine at a density of 1010 cfu/mL injected intramuscularly (i.m.) at a dose of 0.4 mL/kg fish. Nile tilapia was injected with a vaccine used as a treatment. Example include A. hydrophila monovalent (MA), S. agalactiae monovalent (MS), P. fluorescens monovalent (MP), A. hydrophila and S. agalactiae bivalent (BAS), A. hydrophila and P. fluorescens bivalent (BAP), P. fluorescens and S. agalactiae bivalent (BPS), and A. hydrophila, S. agalactiae, and P. fluorescens polyvalent vaccines (PAPS). While the control was fish that were injected with a PBS solution. The broodstock’s immune response was observed on the 7th, 14th, 21st, and 28th days, while the immune response and challenge test on the offspring was conducted on the 10th, 20th, 30th, and 40th day during the post-hatching period. The parameters observed consisted of total leukocytes, phagocytic activity, antibody titer, lysozyme, and relative survival percentage (RPS). Result The application of PAPS in broodstock could significantly induce the best immune response and immunity to multiple diseases compared to other treatments. The RPS of the PAPS was also higher than the other types of vaccines. This showed that the transfer of immunity from the broodstock to the Nile tilapia offspring could protect it against bacterial diseases such as A. hydrophila, S. agalactiae, and P. fluorescens. Conclusion The application of polyvalent vaccine A. hydrophila, S. agalactiae, P. fluorescens vaccines increased the broodstock’s immune response and it was transferred to their offsprings. Polyvalent vaccines derived from maternal immunity can protect offspring from disease up to 30 days of age. They were able to produce tilapia seeds that are immune to diseases caused by A. hydrophila, S. agalactiae, and P. fluorescens.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Transfer of maternal immunity using a polyvalent vaccine and offspring protection in Nile tilapia, Oreochromis niloticus

Amrullah,

Wahidah,

Ardiansyah

et al. 2023

F1000Res

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“…At present, it is believed that psychrophilic bacteria mainly adapt to the low temperature environment through the following mechanisms. Low temperature resistance mechanism: straight-chain and branchedchain unsaturated fatty acids in the lipid components of the membrane can reduce the melting point of lipids, maintain good fluidity, and ensure the nutritional needs of microorganisms by secreting a large amount of extracellular lipase and protease [4]; tRNA: The content of dihydrouracil in psychrophilic bacteria tRNA is high, which helps to maintain the local conformation of tRNA; Enzyme: The molecular structure of low-temperature enzymes has good flexibility, and can quickly adjust conformation for catalytic reactions; Acid-resistant mechanism, metal ions on the surface of the membrane will exchange with hydrogen ions in an acidic environment, so as to avoid excessive hydrogen ions from causing poisonous cells; Transmembrane potential difference: acidophilic microorganisms maintain a neutral environment in the cell through the balance mechanism and the diffusion of hydrogen ions; alkali resistance mechanism: the cell wall contains a large number of acidic small molecules, which can neutralize H+ on the cell surface; cell membrane: resist intracellular pH Value changes, maintain intracellular pH close to neutral; DNA: Some DNA is related to alkali resistance [5].…”

Section: Introductionmentioning

confidence: 99%

Differential analysis of transcriptome of psychrophilic bacteria under different culture temperatures

Xu,

Yang,

Zhu

et al. 2024

THC

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BACKGROUND: Psychrophilic bacteria can survive in a unique living environment. OBJECTIVE: To explore the mechanism of low temperature adaptation and the physiological function of thermophilic metabolic genes. METHOD: Serratia marcescens strain F13 stored in microbial laboratory was cultured at 5∘C, 10∘C and 25∘C respectively, and the obtained strains were sequenced by high-throughput transcriptome. Serratia marcescens strain CAV1761 was used as the reference strain. The data produced by transcriptome sequencing were statistically analyzed by biostatistics software such as soapnuke, soap and edger. The differentially expressed genes were found based on the gene expression, and analyzed by Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. RESULTS: The results showed that there were 718 differential genes in F13-10 vs F13-5 comparison group, 1614 differential genes in F13-25 vs F13-5 comparison group and 1636 differential genes in F13-25 vs F13-10 comparison group. GO function enrichment analysis showed that the GO term mainly enriched by different genes in the three comparison groups was mostly related to the migration and transport of cellular or subcellular components, cell localization and transmembrane transporter activity, as well as cilia or flagella dependent cell movement. In the enrichment analysis of KEGG pathway, the three comparison groups all enriched the largest number of differential genes in the branch pathway of KEGG metabolism, followed by the branch pathway of environmental information processing. CONCLUSION: In F13-10 vs F13-5, the differential genes were mainly concentrated in 20 pathways such as ATP-binding cassette transport (ABC) transporters, thiamine metabolism and flagella assembly; In F13-25 vs F13-5, the differential genes are mainly concentrated in 20 pathways, such as (ABC) transporters, arginine and proline metabolism, two-component system and so on; In F13-25 vs F13-10, the differential genes are mainly concentrated in 20 pathways such as various types of glycan synthesis, two-component system and arginine metabolism.

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“…This process is usually carried out using monovalent vaccines [27][28][29][30] . However, a polyvalent vaccine would be more effective because it could control multiple diseases 3,31,32 especially using a formalin-killed vaccine with low production cost compared to other types of vaccines 3 .…”

Section: Introductionmentioning

confidence: 99%

“…The types of bacterial diseases studied in the aforementioned studies are very limited even though Nile tilapia often suffer from them in fish farms and hatcheries 35 . Besides being infected by S. agalactiae 29,[34][35][36] , Nile tilapia are often infected by A. hydrophila 9,35,37 and P. fluorescens 37,38 leading to high mortality, including in Indonesia. Therefore, this study aimed to examine maternal immunity transfer using the polyvalent vaccine for S. agalactiae, A. hydrophila, and P. fluorescens (PAPS).…”

Section: Introductionmentioning

confidence: 99%

Transfer of maternal immunity using a polyvalent vaccine and offspring protection in Nile tilapia, Oreochromis niloticus

Amrullah¹,

Wahidah²,

Ardiansyah³

et al. 2022

F1000Res

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Background: Vaccination is an effective and alternative means of disease prevention, however, it cannot be conducted on the offspring of fish. For this process to take place, the transfer of maternal immunity must be implemented. This study aims to determine the effectiveness of transferring immunity from the broodstock to the offspring using a polyvalent vaccine against Aeromonas hydrophila, Streptococcus agalactiae, and Pseudomonas fluorescens in Nile tilapia, Oreochromis niloticus. Methods: Nile tilapia broodstock, with an average weight of 203g (±SD 23 g) was injected with a vaccine used as a treatment. Example include A. hydrophila monovalent (MA), S. agalactiae monovalent (MS), P. fluorescens monovalent (MP), A. hydrophila and S. agalactiae bivalent (BAS), A. hydrophila and P. fluorescens bivalent (BAP), P. fluorescens and S. agalactiae bivalent (BPS), and A. hydrophila, S. agalactiae, and P. fluorescens polyvalent vaccines (PAPS). While the control was fish that were injected with a PBS solution. The broodstock’s immune response was observed on the 7th, 14th, 21st, and 28th day, while the immune response and challenge test on the offspring was conducted on the 10th, 20th, 30th, and 40th day during the post-hatching period. Result: The application of PAPS in broodstock could significantly induce the best immune response and immunity to multiple diseases compared to other treatments. The RPS of the PAPS was also higher than the other types of vaccines. This showed that the transfer of immunity from the broodstock to the Nile tilapia offspring could protect it against bacterial diseases such as A. hydrophila, S. agalactiae, and P. fluorescens. Conclusion: The application of PAPS A. hydrophila, S. agalactiae, P. fluorescens vaccines increased the broodstock’s immune response and it was transferred to their offsprings. They were able to produce tilapia seeds that are immune to diseases caused by A. hydrophila, S. agalactiae, and P. fluorescens.

show abstract

Passive protective ability of the outer membrane protein PF1380 of Pseudomonas fluorescens against the major pathogenic bacteria of freshwater aquaculture in fish

Cited by 5 publications

References 32 publications

Transfer of maternal immunity using a polyvalent vaccine and offspring protection in Nile tilapia, Oreochromis niloticus

Transfer of maternal immunity using a polyvalent vaccine and offspring protection in Nile tilapia, Oreochromis niloticus

Differential analysis of transcriptome of psychrophilic bacteria under different culture temperatures

Transfer of maternal immunity using a polyvalent vaccine and offspring protection in Nile tilapia, Oreochromis niloticus

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