Subsurface microbial
(biogenic) methane production is an important
part of the global carbon cycle that has resulted in natural gas accumulations
in many coal beds worldwide. Laboratory studies suggest that complex
carbon-containing nutrients (e.g., yeast or algae extract) can stimulate
methane production, yet the effectiveness of these nutrients within
coal beds is unknown. Here, we use downhole monitoring methods in
combination with deuterated water (D2O) and a 200-liter
injection of 0.1% yeast extract (YE) to stimulate and isotopically
label newly generated methane. A total dissolved gas pressure sensor
enabled real-time gas measurements (641 days preinjection and for
478 days postinjection). Downhole samples, collected with subsurface
environmental samplers, indicate that methane increased 132% above
preinjection levels based on isotopic labeling from D2O,
108% based on pressure readings, and 183% based on methane measurements
266 days postinjection. Demonstrating that YE enhances biogenic coalbed
methane production in situ using multiple novel measurement methods
has immediate implications for other field-scale biogenic methane
investigations, including in situ methods to detect and track microbial
activities related to the methanogenic turnover of recalcitrant carbon
in the subsurface.