Passive Transfer of Contact Sensitivity to Picryl Chloride in Guinea Pigs, with Subcellular Material

Attempts to Transfer Contact Sensitivity Passively with Subcellular Fractions in the Guinea Pig

“…Although the intensity of reactions to picryl chloride were somewhat increased in such ani mals after the injection of extracts of disrupted lymphoid cells from guinea-pigs sensitive to picryl chloride, similar reactions occurred in recipients after injection of material from guinea-pigs sensitive to oxazolone. Discussion The results confirm that the injection of subcellular material from lymph nodes and spleen of guinea-pigs sensitized to picryl chloride may increase the reactivity of guinea-pig skin to picryl chloride in about 50% of guinea-pigs, although the intensity of reaction in these experiments was somewhat less than in those on which a previous report was based (Turk, 1961). This reaction how ever does not appear to be immunologically specific, as similar re actions are given by guinea-pigs receiving similar material from donors sensitized to oxazolone or receiving Freund's complete ad juvant alone.…”

Immunochemistry

“…However, the rheumatoid factor appears to be made in plasma Turk (235) has reported briefly that delayed type sensitivity to picryl chloride, in guinea pigs, can be passively transferred to normal animals by injection of a suspension of broken lymph node or spleen cells from a sensitized animal. The molecular weight decreases on the addition of thiol, but there is no loss of antibody activity.…”