Pasteurellaceae bacteria from the oral cavity of Tasmanian devils (<i>Sarcophilus Harrisii</i>
) show high minimum inhibitory concentration values towards aminoglycosides and clindamycin

Gutman, Nicole; Hansen, Mie Johanne; Bertelsen, Mads F.; Bojesen, Anders Miki

doi:10.1111/lam.12545

Cited by 5 publications

(4 citation statements)

References 18 publications

(37 reference statements)

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“…Aminoglycoside resistance occurs widely in Gram-negative bacteria, usually because of enzymatic inactivation of the drug (Kehrenberg et al, 2005;Gigu ere, 2013). Our results confirm the findings of Gutman et al (2016), who found that resistance occurred intrinsically for aminoglycosides and lincosamides in bacterial species belonging to the family Pasteurellaceae. This highlights that avoiding the use of the aforementioned antibiotics can enable the application of appropriate therapeutic treatments and prevent the spread of antibiotic resistance.…”

supporting

confidence: 90%

“…Escherichia coli ATCC 25922 served as a quality control strain. MIC values were read after 24-h incubation at 37 8C and were defined as the lowest concentration with complete inhibitory action according to the CLSI standards and as described in a previous study (CLSI, 2018a;CLSI, 2018b;Gutman et al, 2016).…”

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confidence: 99%

“…Very limited information is available on the antibiotic susceptibility of F. canicola, and even this is on isolates from Tasmanian devils (Gutman et al, 2016). Frederiksenia is a relatively recently described genus (Korczak et al, 2014), so there are no breakpoints for antibiotic resistance testing.…”

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confidence: 99%

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Detection of Frederiksenia sp. isolated from a cat with nephritis – Short communication

Újvári

Szeredi

Magyar

2020

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In this paper we report the phenotypic and partial genetic characterisation of a novel bacterium strain isolated from a cat with severe nephritis. Multilocus sequence analysis was performed on the 16S rRNA and three housekeeping (recN, rpoB, infB) gene sequences obtained by PCR. In accordance with the results of phenotypic tests, the phylogenetic analyses confirmed the relatedness of the new strain (6036) to the family Pasteurellaceae. On the phylogenetic trees, strain 6036 appeared in a separate branch, closest to that of the type species (Frederiksenia canicola) of the genus Frederiksenia. These two bacteria shared 95.14 and 76.88% identity in their partial 16S rRNA and recN gene sequences, respectively. The rpoB- and infB-based phylogenetic analyses indicated that strain 6036 is most closely related to Bibersteinia trehalosi (with 90.58% identity) and [Haemophilus] felis ATCC 49733 (89.50% identity), respectively. The predicted genome identity values, based on the recN gene sequences, suggested that strain 6036 can be classified into the genus Frederiksenia as a novel species. A PCR method, specific to strain 6036, was developed to allow its rapid and accurate identification and differentiation from F. canicola and other species of Pasteurellaceae. The minimal inhibitory concentrations of 18 antimicrobial agents for strain 6036 were also determined.

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Detection of Frederiksenia sp. isolated from a cat with nephritis – Short communication

Újvári

Szeredi

Magyar

2020

AVet

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“…The bat oral microbiome was dominated by Pasteurellaceae (phylum Proteobacteria), and in some cases, a high relative abundance of bacteria in the families Mycoplasmataceae (in nycterids), Neisseriaceae (in vespertilionids and rhinonycterids), and Streptococcaceae (in pteropodids) was also observed. Although the oral microbiome has received limited attention relative to the gut, several studies have found diverse Pasteurellaceae and Neisseria lineages present in the oral microbiota of animals, including domestic cats (38), marine mammals (55), and Tasmanian devils (41,56). In humans, Pasteurellaceae (genera Haemophilus and Aggregatibacter) and Neisseriaceae (genera Neisseria, Kingella, and Eikenella) play an important role in the formation of supragingival plaque (40).…”

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Ecology and Host Identity Outweigh Evolutionary History in Shaping the Bat Microbiome

et al. 2019

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Recent studies of mammalian microbiomes have identified strong phylogenetic effects on bacterial community composition. Bats (Mammalia: Chiroptera) are among the most speciose mammals on the planet and the only mammal capable of true flight. We examined 1,236 16S rRNA amplicon libraries of the gut, oral, and skin microbiota from 497 Afrotropical bats (representing 9 families, 20 genera, and 31 species) to assess the extent to which host ecology and phylogeny predict microbial community similarity in bats. In contrast to recent studies of host-microbe associations in other mammals, we found no correlation between chiropteran phylogeny and bacterial community dissimilarity across the three anatomical sites sampled. For all anatomical sites, we found host species identity and geographic locality to be strong predictors of microbial community composition and observed a positive correlation between elevation and bacterial richness. Last, we identified significantly different bacterial associations within the gut microbiota of insectivorous and frugivorous bats. We conclude that the gut, oral, and skin microbiota of bats are shaped predominantly by ecological factors and do not exhibit the same degree of phylosymbiosis observed in other mammals. IMPORTANCE This study is the first to provide a comprehensive survey of bacterial symbionts from multiple anatomical sites across a broad taxonomic range of Afrotropical bats, demonstrating significant associations between the bat microbiome and anatomical site, geographic locality, and host identity—but not evolutionary history. This study provides a framework for future systems biology approaches to examine host-symbiont relationships across broad taxonomic scales, emphasizing the need to elucidate the interplay between host ecology and evolutionary history in shaping the microbiome of different anatomical sites.

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Associations between Afrotropical bats, parasites, and microbial symbionts

Lutz

et al. 2018

Preprint

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30Bats are among the most diverse animals on the planet and harbor numerous bacterial, 31 viral, and eukaryotic symbionts. The interplay between bacterial community composition 32 and parasitism in bats is not well understood and may have important implications for 33 studies of similar systems. Here we present a comprehensive survey of dipteran and 34 haemosporidian parasites, and characterize the gut, oral, and skin microbiota of 35 Afrotropical bats. We identify significant correlations between bacterial community 36 composition of the skin and dipteran ectoparasite prevalence across four major bat 37 lineages, as well as links between the oral microbiome and malarial parasitism, suggesting 38 a potential mechanism for host selection and vector-borne disease transmission in bats. In 39 contrast to recent studies of host-microbe phylosymbiosis in mammals, we find no 40 correlation between chiropteran phylogenetic distances and bacterial community 41 dissimilarity across the three anatomical sites, suggesting that host environment is more 42 important than shared ancestry in shaping the composition of bat-associated bacterial 43 communities. 44 45 46 3 SIGNIFICANCE 48 49Animals rely on bacterial symbionts for numerous biological functions, such as digestion 50and immune system development. Increasing evidence suggests that host-associated 51 microbes may play a role in mediating parasite burden. This study is the first to provide a 52 comprehensive survey of bacterial symbionts from multiple anatomical sites across a 53 broad taxonomic range of Afrotropical bats, demonstrating significant associations 54 between the bat microbiome and parasite prevalence. This study provides a framework for 55 future approaches to systems biology of host-symbiont interactions across broad 56 taxonomic scales, emphasizing the interdependence between microbial symbionts and 57 vertebrate health in the study of wild organisms and their natural history. 58 59 60 61 62 63 64 65 66 67 68 69 pathogens (such as West Nile virus, yellow fever, dengue, malaria, etc.), and ultimately 93 imposing selective pressures on human populations -indeed, positive selection of 94 malaria-protective genes can be seen in the human genome (9). Despite the potential 95 significance of such interactions between hosts, microbes, and pathogen-transmitting 96 vectors, they have not been well studied in most wild vertebrate systems. 97 Bats (Mammalia: Chiroptera) are an important system for comparison of the 98 relative contributions of evolutionary and ecological factors driving host-symbiont 99 associations. In addition to being one of the most speciose orders of mammals (second 100 only to the order Rodentia), bats frequently live in large colonies, are long-lived, and 101 volant, granting them access to a wide geographic range relative to their non-volant 102 mammalian counterparts. The associations of diverse eukaryotic parasites (e.g. dipteran 103insects, haemosporidia, helminths) within numerous bat lineages have been well-104 characterized (10-13). Fur...

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Pasteurellaceae bacteria from the oral cavity of Tasmanian devils (Sarcophilus Harrisii ) show high minimum inhibitory concentration values towards aminoglycosides and clindamycin

Cited by 5 publications

References 18 publications

Detection of Frederiksenia sp. isolated from a cat with nephritis – Short communication

Detection of Frederiksenia sp. isolated from a cat with nephritis – Short communication

Ecology and Host Identity Outweigh Evolutionary History in Shaping the Bat Microbiome

Associations between Afrotropical bats, parasites, and microbial symbionts

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