Pasteurellaceae from Colorado Bighorn Sheep Herds

Abstract

ABSTRACT:We compared phenotypic and genotypic characterizations of 88 Pasteurellaceae isolates from bighorn sheep (Ovis canadensis) in the course of exploring epizootiologic relationships. Based on our observations, adopting contemporary taxonomic conventions and using molecular methods to detect and compare Pasteurellaceae may help improve understanding of bighorn respiratory disease epizootiology and management.

How Respiratory Pathogens Contribute to Lamb Mortality in a Poorly Performing Bighorn Sheep (Ovis canadensis) Herd

“…These tumors may facilitate bacterial pathogen persistence in sinuses, leading to a chronic carrier state (Fox et al 2015). This combination of pathogens also has been observed in other free-ranging bighorn sheep herds in Colorado showing similar patterns of poor recruitment (Miller and Wolfe 2011;Miller et al 2013).…”

A Review of Hypothesized Determinants Associated with Bighorn Sheep (Ovis canadensis) Die-Offs

“…A biovariant system of classifying Pasteurellaceae for wildlife work has been developed due to isolates that were not typable using conventional serologic classification systems [172], but utilization of genotype-based methods may be more appropriate for some research questions [211]. Mannheimia haemolytica, Pasteurella multocida, and Bibersteinia (formerly Pasteurella ) trehalosi have been isolated from pneumonic and healthy bighorn sheep over much of their range, although experimental work over the past half century has largely focused on M. haemolytica from presumptive domestic sheep reservoirs [14, 128, 173, 212, 213] (Table 3).…”

Development and validation of a real-time PCR specific for the leukotoxin gene of Bibersteinia trehalosi

“…The specificity of the PCR primers was evaluated using DNA from 59 known isolates that consisted of M. haemolytica ( n = 8), M. glucosida ( n = 18), Mannheimia ruminali s ( n = 8), and B. trehalosi ( n = 25) previously characterized by BOX-PCR 10 and 16S rRNA sequencing ( n = 33) 9 or by 16S rRNA PCR and subsequent amplicon sequencing ( n = 26). Each species included one or more strains from the NCBI nucleotide database PopSet 459931240.…”