Pasteurellosis as Zoonosis

Arashima, Yasutomo; Kumasaka, Kazunari

doi:10.2169/internalmedicine.44.692

Cited by 19 publications

(12 citation statements)

References 7 publications

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“…If this situation continues, there will be no effective antibiotic therapeutic reserve for some bacterial infections. The implications of a large reservoir of multiresistant organisms, particularly P. multocida, which is not host specific, with resistance that is potentially transferable among livestock species are obvious (3,12,24). Therefore, the use of antimicrobial agents in food animals in ways that minimize the emergence of resistance not only in target pathogens but also in zoonotic bacteria is warranted in the future for the protection of public health.…”

Section: Discussionmentioning

confidence: 99%

Isolation, Antimicrobial Resistance, and Virulence Genes of Pasteurella multocida Strains from Swine in China

et al. 2009

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A total of 233 isolates of Pasteurella multocida were obtained from 2,912 cases of clinical respiratory disease in pigs in China, giving an isolation rate of 8.0%. Serogroup A P. multocida isolates were isolated from 92 cases (39.5%), and serogroup D isolates were isolated from 128 cases (54.9%); 12 isolates (5.2%) were untypeable. P. multocida was the fourth most frequent pathogenic bacterium recovered from the respiratory tract, after Streptococcus suis, Haemophilus parasuis, and Escherichia coli. All isolates were characterized for their susceptibilities to 20 antibiotics and the presence of 19 genes for virulence factors (VFs). The frequency of antimicrobial resistance among P. multocida isolates from swine in China was higher than that reported among P. multocida isolates from swine in from other countries, and 93.1% of the isolates showed multiple-drug resistance. There was a progressive increase in the rate of multiresistance to more than seven antibiotics, from 16.2% in 2003 to 62.8% in 2007. The resistance profiles suggested that cephalosporins, florfenicol, and fluoroquinolones were the drugs most likely to be active against P. multocida. Use of PCR showed that colonization factors (ptfA, fimA, and hsf-2), iron acquisition factors, sialidases (nanH), and outer membrane proteins occurred in most porcine strains. The VFs pfhA, tadD, toxA, and pmHAS were each present in <50% of strains. The various VFs exhibited distinctive associations with serogroups: concentrated in serogroup A, concentrated in serogroup D, or occurring jointly in serogroups A and D. These findings provide novel insights into the epidemiological characteristics of porcine P. multocida isolates and suggest that the potential threat of such multiresistant bacteria in food-producing animals should not be neglected.

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Section: Discussionmentioning

confidence: 99%

Isolation, Antimicrobial Resistance, and Virulence Genes of Pasteurella multocida Strains from Swine in China

et al. 2009

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“…Although, vaccines are given to birds against fowl cholera, yet fowl cholera has remain one of the main causes of loss in poultry [12]. P. multocida is also responsible for atrophic rhinitis in swine, snuffles in rabbit, septicaemia haemorhagica ovis in goat, pneumonia in cattle and haemorhagic septicaemia in cattle and buffalo, showing that P. multocida is not host specific [1], [18]. Antibiotics are used to a large extent for the treatment of fowl cholera.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and antibiotic resistance of Pasteurella multocida isolated from chicken in Ado-Ekiti metropolis

Victor¹,

Mathew²,

Oluyege³

et al. 2016

Int. J. Sci. World

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Pasteurella multocida is a poultry bacterial pathogen causing fowl cholera in chicken. The prevalence and antibiotic susceptibility of P. multocida isolates from freshly dead chicken were determined. Ninety seven (97) freshly dead chicken from 23 different farms were analyzed for the presence of P. multocida. Swabs of the trachea and the liver of the necropsied chicken were activated on buffered peptone water and later cultured on blood agar and MacConkey agar. Pure culture of organisms were subjected to cultural and biochemical characterization. In vitro susceptibility of the pure isolates of P. multocida against 12 antimicrobial agents was determined using disk diffusion method. Twelve isolates of P. multocida were recovered from the chicken, with a prevalence of 12.4%. Nine of the isolates were recovered from the trachea and three from the liver. All the 12 isolates recovered from the birds were multi-resistant to the antibiotics used in this research. The antibiogram showed that all the isolates resisted ampicillin, amoxicillin/clavulinate, doxycycline and tylosine. Nitrofuratoin and gentamycin had the best antimicrobial activity with 25% and 50% resistance respectively. The resistance of other antibiotics are: Ofloxacin 75%, Ciprofloxacin 83.3%, Enrofloxacin 75%, Furasol 66.7%, Ceftazidime 91.7% and Cefuroxime 66.7%. This result showed that there is an emergence of multi-resistance in P. multocida, therefore it is important to carry out sensitivity test before administration of antibiotics in order to control fowl cholera.

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“…The protein toxin from Pasteurella multocida (PMT) experimentally induces all of the major symptoms of a number of economically important zoonotic diseases in wild animals and domestic livestock and pets (Foged 1992;Arashima and Kumasaka 2005;Harper et al 2006;Wilson and Ho 2006), including moderate to severe progressive atrophic rhinitis, bite wound dermonecrosis and abscesses, chronic respiratory infection, and decreased overall stature and weight gain. PMT is a 1285-aminoacid protein that binds to and enters mammalian cells via receptor-mediated endocytosis Pettit et al 1993;Dudet et al 1996).…”

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confidence: 99%

The C3 domain ofPasteurella multocidatoxin is the minimal domain responsible for activation of G_q‐dependent calcium and mitogenic signaling

et al. 2008

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The large 1285-amino-acid protein toxin from Pasteurella multocida (PMT) is a multifunctional singlechain polypeptide that binds to and enters eukaryotic cells and acts intracellularly to promote G q and G 12/13 protein-dependent calcium and mitogenic signal transduction. Previous studies indicated that the intracellular activity domain responsible for PMT action was located within the C-terminal 600-700 amino acids. In this study, we have exogenously expressed a series of N-and C-terminal PMT fragments directly in mammalian cells and have used the dual luciferase reporter system to assay for toxinmediated activation of calcium-calcineurin-NFAT signaling (NFAT-luciferase) and mitogenic serum response signaling (SRE-luciferase). Using this approach, we have defined the last 180 amino acids, which encompass the C3 domain in the crystal structure, as the minimum domain sufficient to activate both NFAT and SRE signaling pathways.

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Pasteurellosis as Zoonosis

Cited by 19 publications

References 7 publications

Isolation, Antimicrobial Resistance, and Virulence Genes of Pasteurella multocida Strains from Swine in China

Isolation, Antimicrobial Resistance, and Virulence Genes of Pasteurella multocida Strains from Swine in China

Prevalence and antibiotic resistance of Pasteurella multocida isolated from chicken in Ado-Ekiti metropolis

The C3 domain ofPasteurella multocidatoxin is the minimal domain responsible for activation of G_q‐dependent calcium and mitogenic signaling

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Pasteurellosis as Zoonosis

Cited by 19 publications

References 7 publications

Isolation, Antimicrobial Resistance, and Virulence Genes of Pasteurella multocida Strains from Swine in China

Isolation, Antimicrobial Resistance, and Virulence Genes of Pasteurella multocida Strains from Swine in China

Prevalence and antibiotic resistance of Pasteurella multocida isolated from chicken in Ado-Ekiti metropolis

The C3 domain ofPasteurella multocidatoxin is the minimal domain responsible for activation of Gq‐dependent calcium and mitogenic signaling

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The C3 domain ofPasteurella multocidatoxin is the minimal domain responsible for activation of G_q‐dependent calcium and mitogenic signaling