Native vegetation of the Canterbury Plains of South Island in New Zealand has been heavily modified by agriculture and now occupies less than 0.5% of the total land area. With recent large-scale conversion to intensive dairy farming, restoration of native plants and biodiversity into a modern agricultural matrix creates a significant challenge. Native species are adapted to low nitrogen (N) environments but fertilizers and effluents have substantially raised soil N loadings. We investigated the interactions of selected native species to elevated soil N, using field studies and glasshouse-based nutrient trials. Growth and uptake of N by perennial ryegrass provided a reference. At restoration sites, several native species had similar foliar N concentrations to ryegrass. Deciduous and N-fixing species of tree had highest concentrations. There was significant inter-species variation in soil mineral N concentrations in native plant rhizospheres, differing substantially to the root zone of ryegrass. Pot trials revealed that native species tolerate high N-loadings (up to 1600 kg ha-1), although there was a negligible or no significant growth response. Among the native plants, monocots (tussock grass, sedge and NZ flax) assimilated most N, although total N assimilation by ryegrass would exceed that of native species at field productivity rates. Nevertheless, the deeper rhizospheres of native species may reduce nitrate leaching when planted on the margins of agricultural land or for effluent disposal. Selected native plant species could contribute to the sustainable management of N in intensive agricultural landscapes. Highlights: Native species are tolerant to elevated soil N, with negligible growth response Low-N adapted plants show species-specific traits that include luxury N uptake Differences rhizosphere N pools exist between native species and with ryegrass Planting natives may help to provide sustainable agricultural management of N