pat-4/ILK and unc-112/Mig-2 are required for gonad function in Caenorhabditis elegans

Xu, Xiaojian; Rongali, Sharath C.; Miles, Jonathan; Lee, Kap Duk; Lee, Myeongwoo

doi:10.1016/j.yexcr.2006.01.006

Cited by 21 publications

(19 citation statements)

References 29 publications

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“…Localization of PAT-3 ␤-integrin to the dense bodies and the M-lines in body wall muscle and cell attachment structures in several other cell types has been reported previously (Francis and Waterston, 1985;Gettner et al, 1995). However, this study is the first to describe intracellular localization of PAT-3 ␤-integrin in the myoepithelial sheath, supporting previous observations that PAT-3 ␤-integrin is required for ovulation Xu et al, 2005Xu et al, , 2006. Limited localization of PAT-3 ␤-integrin to the dense bodies in the myoepithelial sheath is a remarkable difference from its localization to both dense bodies and M-lines in the body wall muscle.…”

Section: Discussionsupporting

confidence: 90%

“…PAT-3 ␤-integrin is expressed in a variety of muscle and nonmuscle cells and also involved in cell migration, gonadal morphogenesis, and axon guidance Poinat et al, 2002;Xu et al, 2006). Localization of PAT-3 ␤-integrin to the dense bodies and the M-lines in body wall muscle and cell attachment structures in several other cell types has been reported previously (Francis and Waterston, 1985;Gettner et al, 1995).…”

Section: Discussionmentioning

confidence: 70%

“…Ovulation is perturbed by disturbing functions of several structural proteins, including MUP-2 troponin T (Myers et al, 1996), PAT-10 troponin C (Ono and Ono, 2004), LEV-11 tropomyosin (Ono and Ono, 2004), PAT-3 ␤-integrin , talin (Cram et al, 2003), PAT-4 integrin-linked kinase (Xu et al, 2006), and UNC-112 Mig-2 (Xu et al, 2006). However, overall organization and molecular components of the contractile apparatuses of the myoepithelial sheath are not clearly understood.…”

Section: Introductionmentioning

confidence: 99%

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Structural components of the nonstriated contractile apparatuses in the Caenorhabditis elegans gonadal myoepithelial sheath and their essential roles for ovulation

Ono

2007

Developmental Dynamics

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Ovulation in the nematode Caenorhabditis elegans is regulated by complex signal transduction pathways and cell-cell interactions. Myoepithelial sheath cells of the proximal ovary are smooth muscle-like cells that provide contractile forces to push a mature oocyte into the spermatheca for fertilization. Although several genes that regulate sheath contraction have been characterized, basic components of the contractile apparatuses of the myoepithelial sheath have not been extensively studied. We identified major structural proteins of the contractile apparatuses of the myoepithelial sheath and characterized their nonstriated arrangement. Of interest, integrin and perlecan were found only at the dense bodies, whereas they localized to both dense bodies and M-lines in the striated body wall muscle. RNA interference of most of the myofibrillar components impaired ovulation in a soma-specific manner. Our results provide basic information that helps understanding the mechanism of sheath contraction during ovulation and establishing a new model to study morphogenesis of nonstriated muscle. Developmental Dynamics 236: 1093-1105, 2007.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

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Structural components of the nonstriated contractile apparatuses in the Caenorhabditis elegans gonadal myoepithelial sheath and their essential roles for ovulation

Ono

2007

Developmental Dynamics

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“…Plates were then incubated overnight at RT to induce the expression of dsRNA. Sterility and endomitotic (Emo) phenotypes of RNAi animals were examined using methods described in previous studies [8,20,21]. Well number I-2K18 of the C. elegans chromosome I RNAi library was the source of the ppk-1 (RNAi) bacterial clone.…”

Section: Nematode and Rnai Analysismentioning

confidence: 99%

Role of phosphatidylinositol-4-phosphate 5′ kinase (ppk-1) in ovulation of Caenorhabditis elegans

Guo

Wycuff

et al. 2007

Experimental Cell Research

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During C. elegans ovulation, the somatic gonad integrates signals from germ cells and propels a mature oocyte into the spermatheca for fertilization. Previous work suggests that phosphoinositide signaling plays important roles in C. elegans fertility. To fully understand inositol-1,4,5-trisphosphate (IP 3 ) signaling in ovulation, we have examined the function of phosphatidylinositol-4-phosphate 5′ kinase (PIP5K) in C. elegans. Our results show that the C. elegans PIP5K homolog, ppk-1, is essential for ovulation in C. elegans; ppk-1 is mainly expressed in somatic gonad, and depletion of ppk-1 expression causes defective ovulation, reduced gonad sheath contractility, and sterility. Increased IP 3 signaling compensates for ppk-1 (RNAi)-induced sterility, suggesting that ppk-1 is linked to IP 3 signaling. These results demonstrate that ppk-1 plays an essential role in IP 3 signaling and cytoskeleton organization in somatic gonad.

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“…Mig-2 has been also shown in C. elegans to interact with integrin-linked kinase (ILK) [31] thus being connected to particularly interesting new cysteine-histidine rich protein-1 (PINCH-1) and parvin, forming a stable ternary protein complex at cell-ECM adhesion sites [32] . Although many studies have been performed on the role of ILK in cancer, even proposing that it is a potential anti-cancer therapeutic target [33,34] , little is known with regard to the ILK-interacting partners.…”

Section: Ras Suppressor-1 (Rsu-1) In Metastasismentioning

confidence: 99%

Cancer cell metastasis; perspectives from the focal adhesion

Zacharia

Gkretsi

2015

Adv Mod Oncol Res

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Abstract:In almost all cancers, most patients die from metastatic disease and not from the actual primary tumor. That is why addressing the problem of metastasis is of utmost importance for the successful treatment and improved survival of cancer patients. Metastasis is a complex process that ultimately leads to cancer cells spreading from the tumor to distant sites of the body. During this process, cancer cells tend to lose contact with the extracellular matrix (ECM) and neighboring cells within the primary tumor, and are thus able to invade surrounding tissues. Hence, ECM and the ECM-associated adhesion proteins play a critical role in the metastatic process. This review will focus on recent literature regarding interesting and novel molecules at the cell-ECM adhesion sites, namely migfilin, mitogen-inducible gene-2 (Mig-2) and Ras suppressor-1 (RSU-1), that are also critically involved in cancer cell metastasis, emphasizing on data from experiments performed in vitro in breast cancer and hepatocellular carcinoma cell lines as well as human breast cancer tissue samples.Keywords: apoptosis; breast cancer; cell-matrix adhesions; Fascin-1; hepatocellular carcinoma; invasion; metastasis; migfilin; PINCH-1; PUMA; Ras suppressor-1; VASP Citation: Zacharia LC, Gkretsi V. Cancer cell metastasis; perspectives from the focal adhesions. Adv Mod Oncol Res 2015; 1(1): 2-7; http://dx

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pat-4/ILK and unc-112/Mig-2 are required for gonad function in Caenorhabditis elegans

Cited by 21 publications

References 29 publications

Structural components of the nonstriated contractile apparatuses in the Caenorhabditis elegans gonadal myoepithelial sheath and their essential roles for ovulation

Structural components of the nonstriated contractile apparatuses in the Caenorhabditis elegans gonadal myoepithelial sheath and their essential roles for ovulation

Role of phosphatidylinositol-4-phosphate 5′ kinase (ppk-1) in ovulation of Caenorhabditis elegans

Cancer cell metastasis; perspectives from the focal adhesion

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