Patatin-Related Phospholipase pPLAIIIγ Involved in Osmotic and Salt Tolerance in Arabidopsis

Li, Jianwu; Li, Maoyin; Yao, Shuaibing; Cai, Guangqin; Wang, Xuemin

doi:10.3390/plants9050650

Cited by 11 publications

(6 citation statements)

References 38 publications

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“…Only the phenotypic characteristics of the pPLAIIIγ (pPLAIIIγOE) in each part of organs have not yet been characterized in Arabidopsis. A T-DNA insertional mutant (SALK_088404) has been reported by the same group to have a negligible level of transcripts as determined via quantitative real-time polymerase chain reaction (qRT-PCR) [25,26]. However, in our study, the transcripts were not significantly reduced in the SALK line (Supplementary Figure S1), and an additionally analyzed line (SAIL_832_E01) showed only 35% reduced transcripts compared to the wild type (Supplementary Figure S1).…”

Section: Overexpression Of Pplaiiiγ Affects Anisotropic Cell Elongationcontrasting

confidence: 45%

The Reduced Longitudinal Growth Induced by Overexpression of pPLAIIIγ Is Regulated by Genes Encoding Microtubule-Associated Proteins

Jang

Seo

Lee

2021

Plants

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There are three subfamilies of patatin-related phospholipase A (pPLA) group of genes: pPLAI, pPLAII, and pPLAIII. Among the four members of pPLAIIIs (α, β, γ, δ), the overexpression of three isoforms (α, β, and δ) displayed distinct morphological growth patterns, in which the anisotropic cell expansion was disrupted. Here, the least studied pPLAIIIγ was characterized, and it was found that the overexpression of pPLAIIIγ in Arabidopsis resulted in longitudinally reduced cell expansion patterns, which are consistent with the general phenotype induced by pPLAIIIs overexpression. The microtubule-associated protein MAP18 was found to be enriched in a pPLAIIIδ overexpressing line in a previous study. This indicates that factors, such as microtubules and ethylene biosynthesis, are involved in determining the radial cell expansion patterns. Microtubules have long been recognized to possess functional key roles in the processes of plant cells, including cell division, growth, and development, whereas ethylene treatment was reported to induce the reorientation of microtubules. Thus, the possible links between the altered anisotropic cell expansion and microtubules were studied. Our analysis revealed changes in the transcriptional levels of microtubule-associated genes, as well as phospholipase D (PLD) genes, upon the overexpression of pPLAIIIγ. Overall, our results suggest that the longitudinally reduced cell expansion observed in pPLAIIIγ overexpression is driven by microtubules via transcriptional modulation of the PLD and MAP genes. The altered transcripts of the genes involved in ethylene-biosynthesis in pPLAIIIγOE further support the conclusion that the typical phenotype is derived from the link with microtubules.

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Section: Overexpression Of Pplaiiiγ Affects Anisotropic Cell Elongationcontrasting

confidence: 45%

The Reduced Longitudinal Growth Induced by Overexpression of pPLAIIIγ Is Regulated by Genes Encoding Microtubule-Associated Proteins

Jang

Seo

Lee

2021

Plants

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“…Around the LD regions of the lead SNP, SYN6348, 22 candidate genes were found. Among these, the gene Zm00001eb115140 , which encodes a patatin-like phospholipase protein, has previously been reported to be involved in osmotic and salt tolerance in Arabidopsis [ 35 ]. The genes Zm00001eb115320 and Zm00001eb115330 —which encode a late embryogenesis abundant (LEA) protein and a NAD-dependent epimerase/dehydratase family protein, respectively—have been reported to participate in response to abiotic stresses [ 36 , 37 , 38 ].…”

Section: Resultsmentioning

confidence: 99%

A Combination of a Genome-Wide Association Study and a Transcriptome Analysis Reveals circRNAs as New Regulators Involved in the Response to Salt Stress in Maize

Liu

Zhu

Líu

et al. 2022

IJMS

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Salinization seriously threatens the normal growth of maize, especially at the seedling stage. Recent studies have demonstrated that circular RNAs (circRNAs) play vital roles in the regulation of plant stress resistance. Here, we performed a genome-wide association study (GWAS) on the survival rate of 300 maize accessions under a salt stress treatment. A total of 5 trait-associated SNPs and 86 candidate genes were obtained by the GWAS. We performed RNA sequencing for 28 transcriptome libraries derived from 2 maize lines with contrasting salt tolerance under normal and salt treatment conditions. A total of 1217 highly expressed circRNAs were identified, of which 371 were responsive to a salt treatment. Using PCR and Sanger sequencing, we verified the reliability of these differentially expressed circRNAs. An integration of the GWAS and RNA-Seq analyses uncovered two differentially expressed hub genes (Zm00001eb013650 and Zm00001eb198930), which were regulated by four circRNAs. Based on these results, we constructed a regulation model of circRNA/miRNA/mRNA that mediated salt stress tolerance in maize. By conducting hub gene-based association analyses, we detected a favorable haplotype in Zm00001eb198930, which was responsible for high salt tolerance. These results help to clarify the regulatory relationship between circRNAs and their target genes as well as to develop salt-tolerant lines for maize breeding.

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“…Studies suggest that the activation of MAPK6 causes the phosphorylation of the antiporter Na + /H + SOS1, which contributes to reducing Na + levels in plants [ 85 ]. In contrast, analysis of a pPLAIIIγa knockout mutant in Arabidopsis showed that the plants were sensitive, while overexpression improved the tolerance of the plants to saline stress [ 86 ]. Future studies should be carried out to establish whether pPLAIIIγ responds by modulating other independent pathways to SOS during osmotic stress.…”

Section: Link Between Lipid Second Messengers and Osmotic Stressmentioning

confidence: 99%

Link between Lipid Second Messengers and Osmotic Stress in Plants

Rodas-Junco

Racagni-Di-Palma

Canul-Chan

et al. 2021

IJMS

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Plants are subject to different types of stress, which consequently affect their growth and development. They have developed mechanisms for recognizing and processing an extracellular signal. Second messengers are transient molecules that modulate the physiological responses in plant cells under stress conditions. In this sense, it has been shown in various plant models that membrane lipids are substrates for the generation of second lipid messengers such as phosphoinositide, phosphatidic acid, sphingolipids, and lysophospholipids. In recent years, research on lipid second messengers has been moving toward using genetic and molecular approaches to reveal the molecular setting in which these molecules act in response to osmotic stress. In this sense, these studies have established that second messengers can transiently recruit target proteins to the membrane and, therefore, affect protein conformation, activity, and gene expression. This review summarizes recent advances in responses related to the link between lipid second messengers and osmotic stress in plant cells.

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Patatin-Related Phospholipase pPLAIIIγ Involved in Osmotic and Salt Tolerance in Arabidopsis

Cited by 11 publications

References 38 publications

The Reduced Longitudinal Growth Induced by Overexpression of pPLAIIIγ Is Regulated by Genes Encoding Microtubule-Associated Proteins

The Reduced Longitudinal Growth Induced by Overexpression of pPLAIIIγ Is Regulated by Genes Encoding Microtubule-Associated Proteins

A Combination of a Genome-Wide Association Study and a Transcriptome Analysis Reveals circRNAs as New Regulators Involved in the Response to Salt Stress in Maize

Link between Lipid Second Messengers and Osmotic Stress in Plants

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