Patch Test Reactions to Human Dander in Atopic Dermatitis

In vivo transfection of a cis element ‘decoy’ against signal transducers and activators of transcription 6 (STAT6)-binding site ameliorates IgE-mediated late-phase reaction in an atopic dermatitis mouse model

“…At least 10 fields were examined for each lobe. The number of cells was counted and expressed as the number of cells per mm 2 .…”

“…In atopic dermatitis (AD), the mechanism underlying skin lesion formation has remained unclear except for the delayed hypersensitivity reaction against environmental substances [1][2][3] or the IgE-mediated immediate or late hypersensitivity reaction against environmental substances, food or inhalant allergens. [4][5][6] To clarify the pathomechanism underlying the development of eczematous skin lesions in AD, we established the eczematous skin reaction induced in mice by the intravenous injection of monoclonal anti-dinitrophenyl (DNP)-IgE antibody and subsequent skin testing with 2,4-dinitrofluorobenzene (DNFB) and analyzed these skin reactions as AD mouse model.…”

Patch Test Reactions to the Acetone-Soluble Fraction of Human Dander in Atopic Dermatitis

“…Various authors have reported that a water-soluble frac tion of environmental substances, such as human dander [5][6][7][8] and house dust mites [11][12][13][14][15], induces positive patch test reactions in many patients with atopic dermatitis. Based on these data, it has been argued that water-soluble environmental antigens play a role in the pathogenesis of the skin disease [5.…”

“…It has been reported that patients with atopic dermatitis often show both positive immediate-type skin reactions [1][2][3][4] and positive delayed-type skin reactions [5][6][7][8] to human dander. The human dander antigen utilized for skin testings in the previous studies was a crude human dander [5,6] or a water-soluble fraction of the dander which was defatted by ether [ 1,2] or acetone [3,4,7,8], In these stud ies, the ether-soluble and acetone-soluble fractions of human dander were discarded.…”

“…The human dander antigen utilized for skin testings in the previous studies was a crude human dander [5,6] or a water-soluble fraction of the dander which was defatted by ether [ 1,2] or acetone [3,4,7,8], In these stud ies, the ether-soluble and acetone-soluble fractions of human dander were discarded. From a review of the liter ature, however, it was not clear whether or not the ether-or acetone-soluble fraction of human dander had antigenicity for immediate-type or delayed-type skin reactions.…”