Paternal aging impacts mitochondrial DNA content and telomere length in mouse embryos

Ito, Jun; Kageyama, Mio; Hara, Shunsuke; Sato, Takuya; Shirasuna, Koumei; Iwata, Hisataka

doi:10.1016/j.mito.2022.12.002

Cited by 3 publications

(8 citation statements)

References 38 publications

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“…We obtained 2-cell-stage embryos from superovulated female mice using a previously described method with minor modifications [ 8 ]. Superovulation was induced by intraperitoneal administration of 5 IU follicle-stimulating hormone (FSH; ASKA Animal Health Co., Ltd., Tokyo, Japan), followed by intraperitoneal administration of 5 IU luteinizing hormone (LH; ASKA Animal Health Co., Ltd.) after 46 h. Super-ovulated female mice were subsequently mated with male mice, and the presence of copulation confirmed mating plugs 24 h after LH administration.…”

Section: Methodsmentioning

confidence: 99%

“…The TL of the embryos was measured in duplicate using qPCR according to a previously reported method [ 8 ]. qPCR was performed using the KAPASYBR®FASTqPCR Master Mix (10 µl; Kapa Biosystems) and 0.5 µM of each primer (Forward: 5ʹ-CGGTTTGTTTGGGTTTGGGTTTGGGTTTGGGTTTGGGTT-3ʹ; Reverse: 5ʹ-GGCTTGCCTTACCCTTACCCTTACCCTTACCCTTACCCT-3ʹ) in a CFX Connect TM Real-Time PCR Detection System (Bio-Rad Laboratories).…”

Section: Methodsmentioning

confidence: 99%

“…However, differences have not been observed at the blastocyst stage [ 6 , 7 ]. In contrast, in mice, the mt-cn at the blastocyst stage was significantly lower in aged male parents [ 8 ]. As all mitochondria in the embryos are derived from the maternal parent (oocytes), the effect of paternal factors on mt-cn in embryos is unclear.…”

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confidence: 99%

“…In contrast, we previously reported that the TL of the blastocysts derived from aged C57BL/6N male mice was shorter than that of blastocysts derived from the same male mouse at a younger age. Furthermore, mt-cn and TL were positively correlated in the embryos [ 8 ]. A positive correlation between mt-cn and TL at the blastocyst stage has been reported in cows and pigs [ 8 ].…”

mentioning

confidence: 99%

“…Furthermore, mt-cn and TL were positively correlated in the embryos [ 8 ]. A positive correlation between mt-cn and TL at the blastocyst stage has been reported in cows and pigs [ 8 ]. These reports indicate that TL and mt-cn in embryos change in a coordinated manner in response to certain environmental conditions and stimuli.…”

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confidence: 99%

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Effect of paternal aging and vitrification on mitochondrial DNA copy number and telomere length of mouse blastocysts

ABURADA,

ITO,

INOUE

et al. 2024

J. Reprod. Dev.

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In this study, we examined the effects of paternal aging on the mitochondrial DNA copy number (mt-cn), telomere length (TL), and gene expression in mouse embryos. The effects of vitrification on the mt-cn and TL of the embryos derived from young and aged male parents (YF and AF, respectively) were examined. C57BL/6N male mice were used for embryo production at 13–23 and 50–55 weeks of age. Two-cell stage embryos were collected from the oviducts of superovulated female mice (8–15 weeks old) and cultured for 24 h until the 8-cell stage, followed by embryo vitrification. Fresh and vitrified-warmed embryos were incubated for 2 days until the blastocyst stage, and mt-cn and TL were investigated. The cell-free mitochondrial DNA copy number (cf-mt-cn) in the spent culture medium (SCM) of the embryos was then investigated. RNA sequencing of blastocysts revealed that metabolic pathways, including oxidative phosphorylation and mTOR pathways, were enriched in differentially expressed genes. The mt-cn and TL of AF-derived blastocysts were lower and shorter, respectively, than those of YF-derived blastocysts. Paternal aging did not affect the blastocyst rate after vitrification. Vitrification of the 8-cell stage embryos did not affect the mt-cn of the blastocysts. However, it increased the cf-mt-cn (cell-free mt-cn) in the SCM of both YF- and AF-derived embryos. Vitrification did not affect the TL of either YF- or AF-derived embryos. Thus, paternal aging affected the mt-cn and TL of the embryos, but vitrification did not affect these parameters in either age groups.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Effect of paternal aging and vitrification on mitochondrial DNA copy number and telomere length of mouse blastocysts

ABURADA,

ITO,

INOUE

et al. 2024

J. Reprod. Dev.

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Telomere length determines the mitochondrial copy number in blastocyst-stage embryos

Inoue,

Aoki,

Ito

et al. 2024

Mitochondrion

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Resveratrol intake by males increased the mitochondrial DNA copy number and telomere length of blastocysts derived from aged mice

TERAMOTO,

OKADA,

ABURADA

et al. 2024

J. Reprod. Dev.

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The present study examined whether male resveratrol intake affected mitochondrial DNA copy number (mt-cn) and telomere length (TL) in blastocysts fathered by young and aged male mice. C57BL/6N male mice supplied with water or water containing 0.1 mM resveratrol were used for embryo production at 14-23 and 48-58 weeks of age. Two-cell-stage embryos were collected from the oviducts of superovulated female mice (8-15 weeks old) and cultured for 3 days until the blastocyst stage. Mt-cn and TL levels were measured by real-time polymerase chain reaction. Resveratrol intake did not affect body weight or water consumption. Resveratrol intake increased the expression levels of SIRT1 in the liver, the antioxidative ability of serum, and extended TL in the heart, whereas there was no significant difference in mt-cn in the heart or TL in sperm. The rate of blastocyst development was significantly lower in aged male mice than in younger mice, and resveratrol intake increased the total number of blastocysts derived from both young and aged males. Resveratrol intake did not affect mt-cn or TL in blastomeres of blastocyst-stage embryos derived from young mice, but significantly increased both mt-cn and TL in blastomeres of blastocysts derived from aged fathers. In conclusion, resveratrol intake increased mt-cn and TL levels in blastocysts derived from aged male mice.

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Paternal aging impacts mitochondrial DNA content and telomere length in mouse embryos

Cited by 3 publications

References 38 publications

Effect of paternal aging and vitrification on mitochondrial DNA copy number and telomere length of mouse blastocysts

Effect of paternal aging and vitrification on mitochondrial DNA copy number and telomere length of mouse blastocysts

Telomere length determines the mitochondrial copy number in blastocyst-stage embryos

Resveratrol intake by males increased the mitochondrial DNA copy number and telomere length of blastocysts derived from aged mice

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