Valid measurements are needed to investigate the impact of parental bonding on child health development from a life-course perspective. The aim was to develop and validate a psychometric rating scale, the Parent-to-Infant Bonding Scale (PIBS) to measure bonding in both mothers and fathers. Internal consistency and construct validity were analysed using data from Swedish parents from both clinical (N = 182), and community (N = 122) population samples. Overall, good internal consistency, convergent validity (against the Postpartum Bonding Questionnaire, analysed in the clinical sample), and discriminant validity (against the mental health constructs depressive symptoms and anxiety) appeared. The results indicate good psychometric measurement properties of the PIBS for both mothers and fathers in community and clinical populations. Similarities in PIBS measurement properties between the groups suggest its usefulness for comparisons between mothers and fathers, and for investigating unique and interactive impacts of maternal and paternal bonding on child outcomes using community and clinical cohorts.