Paternity identification in sugarcane polycrosses by using microsatellite markers

Xavier, Mauro Alexandre; Pinto, Luciana Rossini; Fávero, T. M.; Pérecin, Dilermando; Carlini-Garcia, L. A.; Landell, Marcos Guimarães de Andrade

doi:10.4238/2014.march.31.7

Cited by 11 publications

(16 citation statements)

References 18 publications

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“…In non-consolidated active germplasm banks, poly-crosses are commonly adopted due to the high numerical involvement of the parent population. These parents are tested for their potential identity in order to categorize males and females [100].…”

Section: Paternity Analysismentioning

confidence: 99%

“…However, molecular markers permit the identification of the male parent, allowing pedigree reconstruction [5,77,101]. Xavier et al [100] used these techniques to identify the male parent of 41 elite clones from poly-cross families. In this study, SSR primer pairs identified the most likely male parent by determination of markers present in the clone but absent in the female parent.…”

Section: Paternity Analysismentioning

confidence: 99%

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Development and Use of Simple Sequence Repeats (SSRs) Markers for Sugarcane Breeding and Genetic Studies

et al. 2018

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Recently-developed molecular markers are becoming powerful tools, with applications in crop genetics and improvement. Microsatellites, or simple sequence repeats (SSRs), are widely used in genetic fingerprinting, kinship analysis, and population genetics, because of the advantages of high variability from co-dominant and multi-allelic polymorphisms, and accurate and rapid detection. However, more recent evidence suggests they may play an important role in genome evolution and provide hotspots of recombination. This review describes the development of SSR markers through different techniques, and the detection of SSR markers and applications for sugarcane genetic research and breeding, such as cultivar identification, genetic diversity, genome mapping, quantitative trait loci (QTL) analysis, paternity analysis, cross-species transferability, segregation analysis, phylogenetic relationships, and identification of wild cross hybrids. We also discuss the advantages and disadvantages of SSR markers and highlight some future perspectives.

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Section: Paternity Analysismentioning

confidence: 99%

Section: Paternity Analysismentioning

confidence: 99%

Development and Use of Simple Sequence Repeats (SSRs) Markers for Sugarcane Breeding and Genetic Studies

et al. 2018

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“…For example, this method was used to identify 220 open-pollination progeny of Liriodendron spp., of which 49 male parents were identified for 138 progeny [35]. SSR was also used to identify the male parent of 41 elite clones derived from sugarcane polycross families, showing the importance of using molecular marker technology in the identification and confirmation of male parents of high-performance clones in sugarcane breeding programs [36]. In our study, parents of all triploid clones were successfully identified.…”

Section: Discussionmentioning

confidence: 83%

Male Parent Identification of Triploid Rubber Trees (Hevea brasiliensis) and the Mechanism of 2n Gametes Formation

Yao

Long

et al. 2016

Forests

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Eight triploids were screened among offspring of the rubber tree clone GT1 × different clones by flow cytometry and chromosome counting. Twenty-five simple sequence repeat (SSR) markers were screened to identify the origin of 2n gametes, to determine the male parents of these triploids, and to evaluate the mechanism of 2n gamete formation using band configurations and microsatellite DNA allele counting peak ratios (MAC-PR). The results showed that 2n gametes originated from the maternal rubber tree clone GT1, contributing the extra genome copy present in the triploids. It was confirmed that GT1 is able to produce a 2n megagametophyte spontaneously. Many male parents were shown to provide pollen for formation of triploid rubber trees, including clones RRIC 103, Yunyan 277-5, and three other clones. The second division restitution (SDR) was likely the main mechanism involved in formation of megagametophytes in GT1, as the rate of maternal heterozygosity restitution (HR) of all eight triploids varied from 27.78% to 75.00%, with a mean of 51.46%, and all 25 markers varied from 0% to 100%, with a mean of 51.69%. Elucidation of the origin and formation of 2n gametes will help optimize further sexual hybridization of polyploid rubber trees.

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“…Sugarcane can also self-pollenate, particularly if excess pollen is in the vicinity. Several studies utilizing molecular markers have assessed parent identity, percent outcrossing, and the level of self-pollination from sugarcane crossing [5][6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Fidelity of Sugarcane Crosses Assessed with SSR Markers

2020

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Sugarcane (Saccharum hybrids spp.) is a wind-pollinated species that bears very small yet complete flowers. During crossing, a lack of pollen control (trimming off dehisced florets and hot water treatment) may result in both self-pollination (selfs) and unintentional pollination from stray pollen (off-types). Due to this uncertainty, it is important to estimate the percentage of intentional paternal hybridizations to better understand and possibly improve the crossing process. In this study, six pairs of simple sequence repeats (SSR) primers were used to assess the fidelity of 343 bi-parental crosses made at three U.S. breeding stations in 2002, 2003, 2004, 2005, and 2006. By comparing the SSR fingerprints between the two parents and 20 random progenies, three types of progenies were identified, namely, hybrids, selfs, and off-types. Hybrids had only SSR fingerprints found in either parent; selfs had only SSR fingerprints of the maternal parent; and off-types had SSR fingerprints that were not found in either parent. The fidelity of the 343 crosses fluctuated from 100% hybrids to 100% off-types. Multinomial analysis indicated that the ratio of hybrids from intentional hybridization to selfs and off-types fluctuated by year and location. Selfing rates (−30.5%) and off-type rates (−9.2%) were lower in crosses from which superior progenies were selected and advanced through 10-year field evaluation and testing, but off-type rates were not significant indicating that selfing may reduce advancement rates more than the off-types. Hot water treatment reduced off-types by 27% and selfing by 20%. This research demonstrates the utility of SSR markers for measuring hybridization rates and highlights the importance of pollen control measures through trimming and hot water treatment of sugarcane flowers.

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Paternity identification in sugarcane polycrosses by using microsatellite markers

Cited by 11 publications

References 18 publications

Development and Use of Simple Sequence Repeats (SSRs) Markers for Sugarcane Breeding and Genetic Studies

Development and Use of Simple Sequence Repeats (SSRs) Markers for Sugarcane Breeding and Genetic Studies

Male Parent Identification of Triploid Rubber Trees (Hevea brasiliensis) and the Mechanism of 2n Gametes Formation

Fidelity of Sugarcane Crosses Assessed with SSR Markers

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