Path Analysis of Biomarkers of Exposure and Early Biological Effects among Coke-Oven Workers Exposed to Polycyclic Aromatic Hydrocarbons

Abstract

Many host factors or biomarkers are involved in the process of early DNA damage induced by occupational exposure to polycyclic aromatic hydrocarbons (PAH) as seen in coke-oven workers. This paper aimed to identify complicated causal interrelationship of various biomarkers using the path analysis. In this analysis, we included 235 subjects (166 cokeoven workers and 69 nonexposed controls) whose data on the comet assay (e.g., Olive tail moment) and cytogenetic analysis of peripheral blood lymphocytes as well as urinary 1-hydroxypyrene (1-OHP) were available. The path analysis showed that coke-oven exposure and tobacco smoke were both significant predictors of the concentrations of urinary 1-OHP (P < 0.05), with a coefficient of determination of 0.75. The factors having significant influence on the Olive tail moment were in the following order: urinary 1-OHP > XRCC1-exon 9 variant genotype > ERCC2-exon 10 variant genotype > XRCC1-exon 6 variant genotype, with a coefficient of determination of 0.22. The variables of relative importance in influencing on cytokinesis-block micronucleus frequencies were in the following order: coke-oven exposure > urinary 1-OHP > age > mEH3 variant genotype > ERCC2-exon 10 variant genotype > XRCC1-exon 6 variant genotype, with a coefficient of determination of 0.27. These results indicated that exogenous agents, especially the coke-oven exposure, played a more important role than the genotypes in the induction of early genetic damage. In conclusion, the path analysis seemed to be an alternative statistical approach for the ascertainment of complicated association among related biomarkers for the assessment of occupational exposure. (Cancer Epidemiol Biomarkers Prev 2007;16(6):1193 -9)

Suboptimal DNA Repair Capacity Predisposes Coke-Oven Workers To Accumulate More Chromosomal Damages in Peripheral Lymphocytes

“…The results of path analysis revealed that coke-oven exposure was the most influential factor for the levels of urinary 1-OHP and CBMN frequency, which was consistent with our previous findings (23). The XRCC1 Arg399Gln polymorphism is the most influential factor for DRC, which has a greater path coefficient than that of coke work.…”

“…The odds ratio and 95% confidence intervals (CI) were calculated to characterize the association between the coke-oven exposure and DRC using logistic regression with adjustment for age, and smoking and drinking status. The path analysis was used to identify the complicated causal interrelationship of various biomarkers (23). Path analysis is based on simple regression techniques, but it takes the researcher a step beyond the traditional regression analysis (24).…”

“…Path analysis can help us identify which independent variables are theorized to have indirect effects, in addition to direct effects, on the dependent variable, and the path diagram can directly display the complicated relationships among the independent and dependent variables (24). Path analysis was done with the Proc Calis procedure (23,24). First, univariate analysis was conducted for DRC and ln-transformed urinary 1-OHP and CBMN frequency to screen for relevant factors.…”

Otimização e validação de uma metodologia analítica para determinação de 1-hidroxipireno em urina de cortadores de cana-de-açúcar

“…Portanto, superior às razões encontradas por Andrade e colaboradores para MP e HPAs. Isso pode ser explicado pelo fato de, na 10 fuligem, os HPAs ainda não terem sofrido reações significativas de transformação em seus derivados, o que ocorre no material particulado atmosférico [22].…”

“…Os HPAs constituem uma família de compostos caracterizada por possuírem 2 ou mais anéis aromáti-cos condensados [8,9]. Vários HPAs sã0 reconhecidos como agentes mutagênicos/carcinogênicos [10]. O pireno, um HPA de quatro anéis condensados, ocorre em altas concentrações na mistura de HPAs atmosféri-co e é absorvido pelo organismo vivo através da inalação, via oral ou em contato com a pele.…”