Pathobiology of salivary glands

Dardick, Irving; Dardick, Alexander M.; Mackay, Allison; Pastolero, Gemma C.; Gullane, Patrick; Burford-Mason, Aileen P.

doi:10.1016/0030-4220(93)90259-7

Cited by 21 publications

(1 citation statement)

References 22 publications

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“…The right parotid main secretory duct and accompanying marginal mandibular branch of the facial nerve were identified on the surface of the masseter muscle and carefully separated from each other. The main duct was ligated using a micro-clamp, as described previously [22], at a point 5 mm distal to the hilum of the gland. The ligature was retained in place for 2 weeks after which the animals were sacrificed and the parotid gland removed.…”

Section: Duct Ligationmentioning

confidence: 99%

Intracellular calcium signalling in rat parotid acinar cells that lack secretory vesicles

Liu

Scott

Smith

1998

Biochemical Journal

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Secretory vesicles from pancreatic acinar cells have recently been shown to release Ca2+ after stimulation with Ins(1,4,5)P3 [Gerasimenko, Gerasimenko, Belan and Petersen, (1996) Cell 84, 473-480]. These observations have been used in support of the hypothesis that Ca2+ release from secretory vesicles could be an important component of stimulus secretion coupling in exocrine acinar cells. In the rat, ligation of the parotid duct causes a reversible atrophy of the parotid gland. Most notably, after atrophy the acinar cells are reduced in size and no longer contain secretory vesicles [Liu, Smith, and Scott (1996) J. Dent. Res. 74, 900]. We have measured cytosolic free-Ca2+ concentration ([Ca2+]i) in single, acutely isolated, rat parotid acinar cells, and compared Ca2+ mobilization in response to acetylcholine (ACh) stimulation in cells obtained from control animals to that in cells lacking secretory vesicles obtained after atrophy of the parotid gland. Application of 50-5000 nM ACh to control cells gave rise to a typical, dose-dependent, biphasic increase in [Ca2+]i, of which the later, plateau, phase was acutely dependent on the extracellular Ca2+ concentration. An identical pattern of response was observed with cells obtained from atrophic glands. Low concentrations of ACh (10-100 nM) occasionally produced [Ca2+]i oscillations of a similar pattern in cells from both control and atrophic glands. We were able to show that Ca2+ rises first in the apical pole of the cell and the increase then spreads to the rest of the cell in cells from control glands but not in cells from atrophic glands. However, at present we are unable to determine whether this is due to the lack of secretory vesicles or whether the separation is too small to measure in the smaller acinar cells obtained from atrophic glands. We conclude therefore, that secretory vesicles make no significant contribution to overall Ca2+ mobilization in rat parotid acinar cells, nor are they required for oscillatory changes in [Ca2+]i to occur. However we are unable to eliminate completely any role for secretory vesicles in initiating Ca2+ mobilization at the apical pole of the cell.

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Section: Duct Ligationmentioning

confidence: 99%