PathoDNA, an Advanced Diagnostic for Lyme Disease &amp;amp; Co-Infections Utilizing Next Generation DNA Sequencing with Greater Sensitivity and Selectivity Than ELISA/Western Blot

Schettig, Robert; Tan-Lim, Ruth; Warren, D. R.; Poteet, Zachary; Sears, Rory; Hummel, Michael; Coffin, Connor; Quart, Kendra; Aussems, Christopher; Matthias, Ronald; Medina, D. J.; Klein, Matthew Henry; Roberts, Gayle T.; Oertle, John; Prato, Dino

doi:10.4236/aid.2021.114037

Cited by 1 publication

(1 citation statement)

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“…In a new NGS approach, the limitations of the sample sources may be circumvented by using patient urine [ 203 ]. While most NGS-based assays are limited by the low counts of Borreliella genomic material present in human samples such as blood, this approach aims to ensure a higher Borreliella DNA yield with claims that Lb bacteria infect the kidneys.…”

Section: Borreliaceae Diagnosticsmentioning

confidence: 99%

Large-Scale Sequencing of Borreliaceae for the Construction of Pan-Genomic-Based Diagnostics

Socarras

Haslund-Gourley

Cramer

et al. 2022

Genes

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The acceleration of climate change has been associated with an alarming increase in the prevalence and geographic range of tick-borne diseases (TBD), many of which have severe and long-lasting effects—particularly when treatment is delayed principally due to inadequate diagnostics and lack of physician suspicion. Moreover, there is a paucity of treatment options for many TBDs that are complicated by diagnostic limitations for correctly identifying the offending pathogens. This review will focus on the biology, disease pathology, and detection methodologies used for the Borreliaceae family which includes the Lyme disease agent Borreliella burgdorferi. Previous work revealed that Borreliaceae genomes differ from most bacteria in that they are composed of large numbers of replicons, both linear and circular, with the main chromosome being the linear with telomeric-like termini. While these findings are novel, additional gene-specific analyses of each class of these multiple replicons are needed to better understand their respective roles in metabolism and pathogenesis of these enigmatic spirochetes. Historically, such studies were challenging due to a dearth of both analytic tools and a sufficient number of high-fidelity genomes among the various taxa within this family as a whole to provide for discriminative and functional genomic studies. Recent advances in long-read whole-genome sequencing, comparative genomics, and machine-learning have provided the tools to better understand the fundamental biology and phylogeny of these genomically-complex pathogens while also providing the data for the development of improved diagnostics and therapeutics.

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Section: Borreliaceae Diagnosticsmentioning

confidence: 99%