Pathogen-inducible OsMPKK10.2-OsMPK6 cascade phosphorylates the Raf-like kinase OsEDR1 and inhibits its scaffold function to promote rice disease resistance

Ma, Haigang; Li, Juan; Ma, Ling; Wang, Peilun; Xue, Yuan; Yin, Ping; Xiao, Jinghua; Wang, Shiping

doi:10.1016/j.molp.2021.01.008

Cited by 48 publications

(47 citation statements)

References 56 publications

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“…The Raf-like MAPKKK AtEDR1 directly associates with AtMAPKK4/5 and acts as a negative regulator of the AtMAPKK4/5-AtMPK3/6 cascade (Zhao et al, 2014). OsEDR1 directly interacts with OsMAPKK10.2 and negatively regulates the OsMAPKK10.2-OsMAPK6 cascade (Ma et al, 2021). The MEKK-like MAPKKK AtMEKK2 can also serve as a negative modulator of the AtMAPKK1/2-AtMAPK4 cascade by directly interacting with AtMAPK4 (Nitta et al, 2020).…”

Section: Osila1 Is a Negative Modulator Of A Mapk Cascadementioning

confidence: 99%

“…To generate the OsMAPK6-RNAi construct, an OsMAPK6-specific fragment was amplified by PCR using the cDNA above as template and cloned into the pDS2301 vector (Ma et al, 2017). To generate genome-edited osmapkk4 mutants by CRISPR/Cas9, two simple guide RNAs targeting the single exon were ligated into the pCXUN-Cas9 vector (Ma et al, 2021). These constructs were introduced into Agrobacterium (A. tumefaciens) strain EHA105, and the resulting agrobacteria were used to transform rice calli from mature embryos of ZH11 or the osila1 mutant as previously described (Lin and Zhang, 2005).…”

Section: Generation Of Constructs and Rice Transformationmentioning

confidence: 99%

“…Arabidopsis TRANSMEMBRANE KINASE 1 (TMK1) and TMK4 directly interact with and phosphorylate MAPKK4/5 to activate the MAPKK4/5-MAPK3/6 cascade (Huang et al, 2019). Rice EDR1 interacts with OsMAPKK10.2 to inhibit OsMAPKK10.2 activity toward its substrate OsMAPK6 (Ma et al, 2021). DOESN'T MAKE INFECTIONS 3 (OsDMI3), a calmodulindependent protein kinase, directly phosphorylates T25 in the N terminus of OsMAPKK1, which increases its activating phosphorylation of its downstream kinase OsMAPK6 (Chen et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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The rice Raf‐like MAPKKK OsILA1 confers broad‐spectrum resistance to bacterial blight by suppressing the OsMAPKK4–OsMAPK6 cascade

Chen

Wang

Yang

et al. 2021

JIPB

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Mitogen-activated protein kinase kinase kinase (MAPKKK) are the first components of MAPK cascades, which play pivotal roles in signaling during plant development and physiological processes. The genome of rice encodes 75 MAPKKKs, of which 43 are Raf-like MAPKKKs. The functions and action modes of most of the Raf-like MAPKKKs, whether they function as bona fide MAPKKKs and which are their downstream MAPKKs, are largely unknown. Here, we identified the osmapkkk43 mutant, which conferred broadspectrum resistance to Xanthomonas oryzae pv. oryzae (Xoo), the destructive bacterial pathogen of rice. Oryza sativa (Os)MAPKKK43 encoding a Raflike MAPKKK was previously known as Increased Leaf Angle 1 (OsILA1). Genetic analysis indicated that OsILA1 functioned as a negative regulator and acted upstream of the OsMAPKK4-OsMAPK6 cascade in rice-Xoo interactions. Unlike classical MAPKKKs, OsILA1 mainly phosphorylated the threonine 34 site at the N-terminal domain of Os-MAPKK4, which possibly influenced the stability of OsMAPKK4. The N-terminal domain of OsILA1 is required for its homodimer formation and its full phosphorylation capacity. Taken together, our findings reveal that OsILA1 acts as a negative regulator of the OsMAPKK4-OsMAPK6 cascade and is involved in rice-Xoo interactions.

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Section: Osila1 Is a Negative Modulator Of A Mapk Cascadementioning

confidence: 99%

Section: Generation Of Constructs and Rice Transformationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The rice Raf‐like MAPKKK OsILA1 confers broad‐spectrum resistance to bacterial blight by suppressing the OsMAPKK4–OsMAPK6 cascade

Chen

Wang

Yang

et al. 2021

JIPB

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“…OsMPKK10.2-OsMPK6 cascade promotes rice resistance to M. oryzae and X. oryzae pv. oryzicola via the activation of salicylic acid (SA) transduction (Ueno et al 2015;Ma et al 2017Ma et al , 2021. Additionally, several MAPKKK and MAPK genes have been identified in regulating resistance to diseases in rice.…”

mentioning

confidence: 99%

Two VQ Proteins are Substrates of the OsMPKK6-OsMPK4 Cascade in Rice Defense Against Bacterial Blight

Yang

et al. 2021

Rice

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Background The plant-specific valine-glutamine (VQ) protein family with the conserved motif FxxxVQxLTG reportedly functions with the mitogen-activated protein kinase (MAPK) in plant immunity. However, the roles of VQ proteins in MAPK-mediated resistance to disease in rice remain largely unknown. Results In this study, two rice VQ proteins OsVQ14 and OsVQ32 were newly identified to function as the signaling components of a MAPK cascade, OsMPKK6-OsMPK4, to regulate rice resistance to Xanthomonas oryzae pv. oryzae (Xoo). Both OsVQ14 and OsVQ32 positively regulated rice resistance to Xoo. In vitro and in vivo studies revealed that OsVQ14 and OsVQ32 physically interacted with and were phosphorylated by OsMPK4. OsMPK4 was highly phosphorylated in transgenic plants overexpressing OsMPKK6, which showed enhanced resistance to Xoo. Meanwhile, phosphorylated OsVQ14 and OsVQ32 were also markedly accumulated in OsMPKK6-overexpressing transgenic plants. Conclusions We discovered that OsVQ14 and OsVQ32 functioned as substrates of the OsMPKK6-OsMPK4 cascade to enhance rice resistance to Xoo, thereby defining a more complete signal transduction pathway for induced defenses.

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“…These indicated the larger biomass and more grains in the sbm1 mutant genetically depended on OsMPK6 . Given that OsMPK6 was also responsible for resistance to abiotic and biotic stresses (Ma et al ., 2021 ) and its homologous gene MPK6 in Arabidopsis could modulate nitrate reductase activity (Wang et al ., 2011 ), the significance of the SBM1‐OsMPK6 mediated pathway was highlighted, and the function of SBM1 on yield performance and nitrogen utilization described above were further confirmed.…”

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confidence: 99%

The SEEDLING BIOMASS 1 allele from indica rice enhances yield performance under low‐nitrogen environments

Shang

Wang

et al. 2021

Plant Biotechnology Journal

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Pathogen-inducible OsMPKK10.2-OsMPK6 cascade phosphorylates the Raf-like kinase OsEDR1 and inhibits its scaffold function to promote rice disease resistance

Cited by 48 publications

References 56 publications

The rice Raf‐like MAPKKK OsILA1 confers broad‐spectrum resistance to bacterial blight by suppressing the OsMAPKK4–OsMAPK6 cascade

The rice Raf‐like MAPKKK OsILA1 confers broad‐spectrum resistance to bacterial blight by suppressing the OsMAPKK4–OsMAPK6 cascade

Two VQ Proteins are Substrates of the OsMPKK6-OsMPK4 Cascade in Rice Defense Against Bacterial Blight

The SEEDLING BIOMASS 1 allele from indica rice enhances yield performance under low‐nitrogen environments

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